Optimization of a syngeneic murine model of bone metastasis

Farhoodi, Henry P.; Ségaliny, Aude I.; Wagoner, Zachary W.; Cheng, Jason L.; Liu, Linan; Zhao, Weian

doi:10.1016/j.jbo.2020.100298

Cited by 12 publications

(14 citation statements)

References 24 publications

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“…Immortalized human breast cancer cell lines, such as MDA-MB-231, 4T1, and MCF-7, are more easily available than patient-derived tissues. They possess obvious breast cancer target characteristics, and can also exhibit a tendency for bone metastasis after multiple passages ( Table 1 ) ( 2 , 5 , 11 , 51 ). They can help restore human bone metastasis in animal models.…”

Section: Cancer Cell Linesmentioning

confidence: 99%

“…Once its incidence was confirmed, the mice were sacrificed to collect the metastatic tumor cells from the leg bones. These cells were cultivated to purify tumor cells with bone-metastatic tendencies ( 51 ). They purified their experimental cells to improve the success rate.…”

Section: Cancer Cell Linesmentioning

confidence: 99%

“…Intra-arterial injections are usually administered to the left ventricle, limiting the clearance of cells that occurs when they pass through the lung capillaries ( 10 , 53 , 67 ). Tail vein injection, which is the more common intravenous injection today, effectively increases the rate of bone metastasis while also increasing the rate of mortality in mice ( 51 ).…”

Section: Preparation Of Cell Lines For Transplantationmentioning

confidence: 99%

“…Caudal vascular injections can either be intravenous or arterial. Injecting through the tail artery will reduce the elimination of tumor cells in pulmonary capillaries and improve the success rate of colonization to the bone, while tail vein injection will promote tumor metastasis to the lung ( 2 , 51 , 74 ). In Farhoodi’s experiments, the 4T1 cell model tail artery injection mice showed a significant number of tumor cells localized to the subinguinal fat pad and the leg bone ( 51 ).…”

Section: Preparation Of Cell Lines For Transplantationmentioning

confidence: 99%

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Animal models of cancer metastasis to the bone

Peng

et al. 2023

Front. Oncol.

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Cancer metastasis is a major cause of mortality from several tumors, including those of the breast, prostate, and the thyroid gland. Since bone tissue is one of the most common sites of metastasis, the treatment of bone metastases is crucial for the cure of cancer. Hence, disease models must be developed to understand the process of bone metastasis in order to devise therapies for it. Several translational models of different bone metastatic tumors have been developed, including animal models, cell line injection models, bone implant models, and patient-derived xenograft models. However, a compendium on different bone metastatic cancers is currently not available. Here, we have compiled several animal models derived from current experiments on bone metastasis, mostly involving breast and prostate cancer, to improve the development of preclinical models and promote the treatment of bone metastasis.

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Section: Cancer Cell Linesmentioning

confidence: 99%

Section: Cancer Cell Linesmentioning

confidence: 99%

Section: Preparation Of Cell Lines For Transplantationmentioning

confidence: 99%

Section: Preparation Of Cell Lines For Transplantationmentioning

confidence: 99%

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Animal models of cancer metastasis to the bone

Peng

et al. 2023

Front. Oncol.

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“…Approaches to colonize the bone in mouse models are typically divided into direct injection of a long bone such as a femur or tibia or injections into the heart or femoral artery can avoid the destruction and injury to trabecular bone. However, models of bone metastasis through the circulatory system face challenges for cells to engraft in the bone due to the high pressure of arterial circulation (29). A novel approach to model bone metastasis using a caudal artery injection in the tail of mice is able to utilize this arterial vasculature pressure to specifically flood the hind limbs of mice accurately and without causing an artificial injury to the bone (24).…”

Section: Caudal Artery Injected Human Pc3 Osteolytic Prostate Cancer Cells Have Reduced Engraftment With Bmp Inhibitionmentioning

confidence: 99%

Targeting the BMP Pathway in Prostate Cancer Induced Bone Disease

et al. 2021

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From the 33,000 men in the U.S. who die from prostate cancer each year, the majority of these patients exhibit metastatic disease with bone being the most common site of metastasis. Prostate cancer bone metastases are commonly blastic, exhibiting new growth of unhealthy sclerotic bone, which can cause painful skeletal related events. Patient’s current care entails androgen deprivation therapy, anti-resorptive agents, radiation, and chemotherapy to help control the spread of the cancer but little intervention is available to treat blastic bone disease. The transforming growth factor beta (TGFβ) and bone morphogenetic protein (BMP) pathways are known to regulate bone growth and resorption of destructive lytic bone lesions, yet the role of TGFβ/BMP signaling in prostate cancer blastic vs lytic bone lesions are not fully understood. We hypothesized that to target the BMP/TGFβ pathway, a useful biomarker of bone lytic or blastic pathology would have superior response. We show distinct BMP vs. TGFβ signaling in clinical samples of human prostate cancer bone metastases with either lytic or blastic pathologies. BMPs exhibit distinct effects on bone homeostasis, so to examine the effect of BMP inhibition on healthy bone, we treated mice with the BMP receptor small molecule antagonist DMH1 and saw a modest temporary improvement in bone health, with increased trabecular bone. We next sought to use the BMP inhibitor DMH1 to treat bone metastasis engraftment seeded by a caudal artery injection of the lytic human prostate cell line PC3 in immunodeficient mice. The colonization by PC3 cells to the bone were restricted with DMH1 treatment and bone health was importantly preserved. We next proceeded to test BMP inhibition in an injury model of established bone metastasis via intratibial injection of the MYC-CaP mouse prostate cell line into FVBN syngeneic mice. DMH1 treated mice had a modest decrease in trabecular bone and reduced lymphocytes in circulation without affecting tumor growth. Taken together we show unique responses to BMP inhibition in metastatic prostate cancer in the bone. These studies suggest that profiling bone lesions in metastatic prostate cancer can help identify therapeutic targets that not only treat the metastatic tumor but also address the need to better treat the distinct tumor induced bone disease.

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Self‐Delivered Supramolecular Nanomedicine with Transformable Shape for Ferrocene‐Amplified Photodynamic Therapy of Breast Cancer and Bone Metastases

Qin

Tong

Zhang

et al. 2021

Adv Funct Materials

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Although nanomedicines can passively target tumor through the enhanced permeability and retention (EPR) effect, their distribution and retention are limited by complex tumor microenvironment. Herein, a self-delivery supramolecular nanoplatform with shape-transforming capacity (Ce6-CD/ Fc-pep-PEG) is constructed by the host-guest interaction between chlorin e6-conjugated β-cyclodextrin (Ce6-CD) and ferrocene-modified FFVLG 3 C-PEG conjugates (Fc-pep-PEG). Following passive accumulation mediated by the EPR effect, hydrophobic Fc is oxidized to water-soluble Fc + by endogenous ROS in tumor sites. The resulting Fc + -pep-PEG fragment dissociated from Ce6-CD and recombined to nanofibers through the intermolecular hydrogen bonds among FFVLG 3 C peptide chains, thus enhancing the retention. Meanwhile, the Ce6-CD fragment still maintained the form of spherical micelles with a relatively smaller size to penetrate into the deep tumor regions. Moreover, the cascade Fenton reaction catalyzed by Fc generated •OH and O 2 to relieve hypoxia and amplify PDT efficiency. In turn, ROS generated by PDT promoted shape-transformation and continuous occurrence of Fenton reaction. In vitro and in vivo evaluations verify that through the positive feedback loop, Ce6-CD/ Fc-pep-PEG can induce a potent antitumor immune response and achieve ROS-potentiated elimination of primary tumor and bone metastasis.

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Optimization of a syngeneic murine model of bone metastasis

Cited by 12 publications

References 24 publications

Animal models of cancer metastasis to the bone

Animal models of cancer metastasis to the bone

Targeting the BMP Pathway in Prostate Cancer Induced Bone Disease

Self‐Delivered Supramolecular Nanomedicine with Transformable Shape for Ferrocene‐Amplified Photodynamic Therapy of Breast Cancer and Bone Metastases

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