Optimization of affinity partitioning conditions of papain in aqueous two-phase system using response surface methodology

Ling, Yuan-Qing; Nie, Huali; Su, Sai-Nan; Branford-White, Christopher; Zhu, Li‐Min

doi:10.1016/j.seppur.2010.04.020

Cited by 38 publications

(27 citation statements)

References 27 publications

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“…However, when the experimental region of interest is cuboidal rather than rotatable/ spherical or when it is preferable to maintain low and high factor levels, and still carry out a RSM design, the CCF or face-centered cube is a feasible alternative involving α = ± 1 [9,13], which is commonly used for the optimization of PEG-salt ATPS [2,16,17,22,25,28,31,34,36,[49][50][51][52][53][54][55][56][57]. For instance, in the CCF design for three factors (e.g., x 1 , x 2 , and x 3 ) the star/axial points are located on the centers of the faces of a cube or hypercube (Fig.…”

Section: Accepted M Manuscriptmentioning

confidence: 99%

“…Since screening is often initiated by FFD/fFD, an optimization is usually performed by using CCD or CCC/CCF designs because they are more tractable, so that screening designs can be easily converted into CCC/CCF optimization designs [13]. Furthermore, the CCD is an effective design usually not requiring many experimental points, and consequently reducing the overall cost [51,60].…”

Section: Accepted M Manuscriptmentioning

confidence: 99%

“…For that purpose, the obtained quadratic/second-order polynomial equation for predicting optimal values in PEG-salt ATPS has to be solved analytically by a statistical regression analysis or response surface regression procedure according to eq 2 [7,10,16,34,51,62,63].…”

Section: Analysis Of Modelmentioning

confidence: 99%

“…Moreover, eq 2 can be analyzed graphically by using three-dimensional response surface plots or contour plots in order to evaluate the optmkimal values [7]. An analysis of these plots is providing a method to reveal the interactions between two significant factors, all their combinations and one studied response variable in order to determine the most efficient conditions for an optimal target response variable [6,10,14,16,19,37,51,64]. Thereby, three-dimensional response surface plots indicate the effects of operating factors on a response variable, while contour plots represent the nature and magnitude of these effects [15].…”

Section: Analysis Of Modelmentioning

confidence: 99%

“…16 decreasing index of the reliability of a result [15,22,33,51,65]. In other words, ANOVA is used as an important statistical tool in the MLR analysis based on the identification of a regression model, in which the residual sum of squares of the response variable is reduced [13].…”

Section: Accepted M Manuscriptmentioning

confidence: 99%

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Optimization of PEG–salt aqueous two-phase systems by design of experiments

Glyk

Solle

Scheper

et al. 2015

Chemometrics and Intelligent Laboratory Systems

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Since the mechanism governing the partitioning behavior of biomolecules, such as proteins and enzymes, in polyethylene glycol (PEG)-salt aqueous two-phase systems (ATPS) is complex and not easily predictable, many laborious experiments have to be performed for an optimization of these systems, causing increased overall cost. However, the multivariate statistical design of experiments (DoE) methodology is representing a promising and efficient optimization technique which can overcome the limitations of traditional optimization methods. Therefore, DoE has emerged as a powerful and efficient optimization tool for PEGsalt ATPS, since it is faster, more efficient and cost-effective, allowing a simultaneous and rigorous evaluation of process/system parameters. In the present review, different DoE process steps are represented to highlight the feasibility of this approach to operate as a promising and efficient optimization tool, thus facilitating the evaluation of the partitioning behavior, recovery and purification of different proteins and enzymes in PEG-salt ATPS. In this context, several experimental designs, such as factorial and response surface designs, have been discussed and evaluated by statistical regression analysis and analysis of variance (ANOVA), as well as various applications of PEG-salt ATPS using DoE have been outlined which may further promote the optimization of these systems.

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Section: Accepted M Manuscriptmentioning

confidence: 99%

Section: Accepted M Manuscriptmentioning

confidence: 99%

Section: Analysis Of Modelmentioning

confidence: 99%

Section: Analysis Of Modelmentioning

confidence: 99%

Section: Accepted M Manuscriptmentioning

confidence: 99%

See 3 more Smart Citations

Optimization of PEG–salt aqueous two-phase systems by design of experiments

Glyk

Solle

Scheper

et al. 2015

Chemometrics and Intelligent Laboratory Systems

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Optimization of Rhizopus niveus Lipase Partitioning by an Aqueous Biphasic System

et al. 2014

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An unconventional aqueous two‐phase system (ATPS) composed of polyethylene glycol (PEG) and sodium carbonate was developed and optimized by employing response surface methodology for separation of Rhizopus niveus lipase. A five‐level central composite design was applied to evaluate the optimal level of three process variables in order to obtain maximum lipase separation. Experimental data were analyzed by regression and a polynomial model was created which was found significant. The maximum partition coefficient was achieved with the system PEG 4000/sodium carbonate. Validation experiments confirmed the high accordance of predicted and experimental results. The optimized ATPS can be applied as a suitable cost‐effective system for lipase extraction.

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Low‐abundant protein extraction from complex protein sample using a novel continuous aqueous two‐phase systems device

Vázquez-Villegas¹,

Espitia-Saloma²,

Rito‐Palomares³

et al. 2012

J of Separation Science

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The present work describes the application of a novel continuous aqueous two-phase system prototype for the recovery of biomolecules. The prototype is an alternative platform for protein recovery and α-amylase from soybean extracts was used as a model system. The system was selected as an example of low-abundant protein present in complex mixtures. Compared with batch systems, continuous operation in this prototype seems to increase partition coefficient with higher recovery efficiencies. Processing time is reduced at least three times in the continuous system when compared to batch mode, while hold up (volumetric quantity of the opposing phase in a determined phase sample) decreases with decreasing phases flow. Furthermore, similar partition coefficient (Kp > 4) with a higher top phase enzyme recovery (81%) is also obtained in this system probably due to better contact surface between phases, compared with that obtained in batch (79%). A continuous aqueous two-phase system process with purification factor 40-fold higher than batch experiments was achieved. These preliminary results exhibit the potential of continuous systems for the recovery of low-abundant proteins from complex mixtures. The promising performance of this prototype can raise the attention of the industry for the adoption of aqueous two-phase system processes.

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Optimization of affinity partitioning conditions of papain in aqueous two-phase system using response surface methodology

Cited by 38 publications

References 27 publications

Optimization of PEG–salt aqueous two-phase systems by design of experiments

Optimization of PEG–salt aqueous two-phase systems by design of experiments

Optimization of Rhizopus niveus Lipase Partitioning by an Aqueous Biphasic System

Low‐abundant protein extraction from complex protein sample using a novel continuous aqueous two‐phase systems device

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