Optimization of Alkaline Protease Production by &lt;I&gt;Bacillus&lt;/I&gt; sp. Using Taguchi Methodology

Prakasham, R. S.; Rao, Ch. Subba; Rao, R. Srinivasa; Rajesham, S.; Sarma, P.N.

doi:10.1385/abab:120:2:133

Cited by 42 publications

(28 citation statements)

References 20 publications

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“…The enzyme production by microbial strains strongly depends on the initial pH of the medium as it influences many enzymatic processes and transport of various components across the cell membrane [25]. In the present study, xylanase production was noticed in the wide range of pH 3.0 to 9.0 (Table 2) and high xylanase production (2,012 ± 18 U/g) with poor cellulase activity (1.64 ± 0.12 U/g) was achieved at pH 6.0.…”

Section: Effect Of Ph and Temperaturementioning

confidence: 99%

Optimization of upstream and downstream process parameters for cellulase-poor-thermo-solvent-stable xylanase production and extraction by Aspergillus tubingensis FDHN1

Adhyaru

Bhatt

Modi

2015

Bioresour. Bioprocess.

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Background Xylanases are important members of the hemicellulolytic enzyme system. Xylanase plays a vital role in the hydrolysis of major hemicellulosic component xylan and converts it into xylooligosaccharides and ultimately yields xylose. Cellulase-lacking or cellulase-poor xylanase with high temperature and pH stability has gained special attention, especially in paper and pulp industries. Most of the available literature highlighted the fungal xylanase production by optimizing environmental and cultural parameters. However, the importance of enzyme recovery from fermented biomass still needs attention. In this study, upstream and downstream process parameters were studied for enhancing xylanase production and extraction by a newly isolated Aspergillus tubingensis FDHN1 under solid-state fermentation using low-cost agro-residues. Results In the present study, A. tubingensis FDHN1 was used for the xylanase, with very low level of cellulase, production under solid-state fermentation (SSF). Among various agro-residues, sorghum straw enhanced the xylanase production. Under optimized upstream conditions, the highest xylanase production 2,449 ± 23 U/g was observed. Upon characterization, crude xylanase showed stability over a broad range of pH 3.0 to 8.0 up to 24 h. The temperature stability revealed the nature of the xylanase to be thermostable. Native polyacrylamide gel electrophoresis (native PAGE) and zymogram analysis revealed the multiple forms of the xylanase. Due to the many industrially important characteristics of the xylanases, the study was elaborated for optimizing the downstream process parameters such as volume of extractant, extraction time, temperature and agitation speed to recover maximum xylanase from fermented sorghum straw. The highest amount of xylanase (4,105 ± 22 U/g) was recovered using 0.05 M sodium citrate buffer (pH 6.5) at 12:1 (v/w) extractant/solid ratio, 90-min extraction time, 150-rpm agitation speed and 40°C. Finally, detailed bioprocess optimization shows an overall 6.66-fold enhancement in the xylanase yield. Conclusions The present study consolidates the importance of upstream and downstream process optimization for the overall enhancement in the xylanase production. The xylanase from A. tubingensis FDHN1 shows the stability at different pH and temperature, and it was also active in the presence of organic solvents. These properties of xylanase are very much important from an industrial application point of view.

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Section: Effect Of Ph and Temperaturementioning

confidence: 99%

Optimization of upstream and downstream process parameters for cellulase-poor-thermo-solvent-stable xylanase production and extraction by Aspergillus tubingensis FDHN1

Adhyaru

Bhatt

Modi

2015

Bioresour. Bioprocess.

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“…The enzyme production by microbial strains strongly depends on the initial culture pH, as it influences many enzymatic processes and transport of various components across the cell membranes [25]. The influence of pH on xylanase production was studied by adjusting the initial medium (MS medium) pH from 3 to 9 (Fig.…”

Section: Effect Of Particle Size Of Wheat Straw On Xylanase Productionmentioning

confidence: 99%

Production of xylanase under solid-state fermentation by Aspergillus tubingensis JP-1 and its application

Pandya¹,

Gupte²

2012

Bioprocess Biosyst Eng

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The production of extracellular xylanase by a locally isolated strain of Aspergillus tubingensis JP-1 was studied under solid-state fermentation. Among the various agro residues used wheat straw was found to be the best for high yield of xylanase with poor cellulase production. The influence of various parameters such as initial pH, moisture, moistening agents, nitrogen sources, additives, surfactants and pretreatment of substrates were investigated. The production of the xylanase reached a peak in 8 days using untreated wheat straw with modified MS medium, pH 6.0 at 1:5 moisture level at 30 °C. Under optimized conditions yield as high as 6,887 ± 16 U/g of untreated wheat straw was achieved. Crude xylanase was used for enzymatic saccharification of agro-residues like wheat straw, rice bran, wheat bran, sugarcane bagasse and industrial paper pulp. Dilute alkali (1 N NaOH) and acid (1 N H(2)SO(4)) pretreatment were found to be beneficial for the efficient enzymatic hydrolysis of wheat straw. Dilute alkali and acid-pretreated wheat straw yielded 688 and 543 mg/g reducing sugar, respectively. Yield of 726 mg/g reducing sugar was obtained from paper pulp after 48 h of incubation.

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“…For effective decolorization it is essential to optimize the composition and conditions of culture media (Prakasham et al 2005). In conventional methods numerous experiments have to be carried out to optimize all the parameters and establish best possible culture conditions by correlating all the parameters.…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of alkalotolerant bacteria and optimization of process parameters for decolorization and detoxification of pulp and paper mill effluent by Taguchi approach

Mishra

Thakur

2010

Biodegradation

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Four different bacterial strains were isolated from pulp and paper mill sludge in which one alkalotolerant isolate (LP1) having higher capability to remove color and lignin, was identified as Bacillus sp. by 16S RNA sequencing. Optimization of process parameters for decolorization was initially performed to select growth factors which were further substantiated by Taguchi approach in which seven factors, % carbon, % black liquor, duration, pH, temperature, stirring and inoculum size, at two levels, applying L-8 orthogonal array were taken. Maximum color was removed at pH 8, temperature 35°C, stirring 200 rpm, sucrose (2.5%), 48 h, 5% (w/v) inoculum size and 10% black liquor. After optimization 2-fold increase in color and lignin removal from 25-69% and 28-53%, respectively, indicated significance of Taguchi approach in decolorization and delignification of lignin in pulp and paper mill effluent. Enzymes involved in the process of decolorization of effluent were found to be xylanase (54 U/ml) and manganese peroxidase (28 U/ml). Treated effluent was also evaluated for toxicity by Comet assay using Saccharomyces cerevisiae MTCC 36 as model organism, which indicated 58% reduction after treatment by bacterium.

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Optimization of Alkaline Protease Production by <I>Bacillus</I> sp. Using Taguchi Methodology

Cited by 42 publications

References 20 publications

Optimization of upstream and downstream process parameters for cellulase-poor-thermo-solvent-stable xylanase production and extraction by Aspergillus tubingensis FDHN1

Optimization of upstream and downstream process parameters for cellulase-poor-thermo-solvent-stable xylanase production and extraction by Aspergillus tubingensis FDHN1

Production of xylanase under solid-state fermentation by Aspergillus tubingensis JP-1 and its application

Isolation and characterization of alkalotolerant bacteria and optimization of process parameters for decolorization and detoxification of pulp and paper mill effluent by Taguchi approach

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