OPTIMIZATION OF ALKALINE PROTEASE PRODUCTION BY &lt;i&gt;STREPTOMYCES AMBOFACIENS&lt;/i&gt; IN FREE AND IMMOBILIZED FORM

Abdelwahed, Nayera A. M.; Danial, Enas N.; El‐Naggar, Noura El‐Ahmady; Mohamed, Asem A.

doi:10.3844/ajbbsp.2014.1.13

Cited by 20 publications

(8 citation statements)

References 23 publications

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“…Indeed, simple substrates such as casein and gelatin have a low solubility and give low enzyme activity yields in liquid medium. Many microbial protease production studies in liquid media have confirmed our results (Joo and Chang, 2005;Laxman et al, 2005;Tari et al, 2006;Chi et al, 2007;Abidi et al, 2008;Hajji et al, 2008 ;Abdelwahed et al, 2014). In addition, Lazim et al (2009) showed that the addition of casein in the solid culture medium containing wheat bran did not increase the protease production by the Streptomyces sp.…”

Section: Selection Of the Significant Factors For Protease Productionsupporting

confidence: 83%

Optimization of alkaline protease production by Streptomyces sp. strain isolated from saltpan environment

Boughachiche¹,

Rachedi²,

Duran³

et al. 2016

Afr. J. Biotechnol.

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Proteolytic activity of a Streptomyces sp. strain isolated from Ezzemoul saltpans (Algeria) was studied on agar milk at three concentrations. The phenotypic and phylogenetic studies of this strain show that it represents probably new specie. The fermentation is carried out on two different media, prepared at three pH values. The results showed the presence of an alkaline protease with optimal pH and temperature of 8 and 40°C, respectively. The enzyme is stable up to 90°C, having a residual activity of 79% after 90 min. The enzyme production media are optimized according to statistical methods while using two plans of experiences. The first corresponds to the matrixes of Plackett and Burman in N=16 experiences and N-1 factors, twelve are real and three errors. The second is the central composite design of Box and Wilson. The analysis of the results allowed the selection of two factors having a significant effect on the production of the enzyme (fructose and malt extract), then defining theirs optima (7 g/l of fructose and 12 g/l of malt extract).

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Section: Selection Of the Significant Factors For Protease Productionsupporting

confidence: 83%

Optimization of alkaline protease production by Streptomyces sp. strain isolated from saltpan environment

Boughachiche¹,

Rachedi²,

Duran³

et al. 2016

Afr. J. Biotechnol.

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“…Various techniques of cell immobilization are also useful in the production of enzymes such as α-amylase, β-amylase, xylanase, glucoamylase, pullulanase or alkaline phosphatase [13,90,91]. Viable, but non-growing immobilized Escherichia coli cells in biocatalytic films developed by Lyngberg et al [53] produced β-galactosidase with higher specific activity compared to suspended cells.…”

Section: Metabolic Responses To Immobilizationmentioning

confidence: 99%

Metabolic Responses of Bacterial Cells to Immobilization

2016

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In recent years immobilized cells have commonly been used for various biotechnological applications, e.g., antibiotic production, soil bioremediation, biodegradation and biotransformation of xenobiotics in wastewater treatment plants. Although the literature data on the physiological changes and behaviour of cells in the immobilized state remain fragmentary, it is well documented that in natural settings microorganisms are mainly found in association with surfaces, which results in biofilm formation. Biofilms are characterized by genetic and physiological heterogeneity and the occurrence of altered microenvironments within the matrix. Microbial cells in communities display a variety of metabolic differences as compared to their free-living counterparts. Immobilization of bacteria can occur either as a natural phenomenon or as an artificial process. The majority of changes observed in immobilized cells result from protection provided by the supports. Knowledge about the main physiological responses occurring in immobilized cells may contribute to improving the efficiency of immobilization techniques. This paper reviews the main metabolic changes exhibited by immobilized bacterial cells, including growth rate, biodegradation capabilities, biocatalytic efficiency and plasmid stability.

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“…have been reported to produce high levels of alkaline protease in culture media with pH ranging between 7 and 8.5. [33,35] Thermal stability…”

Section: ãããmentioning

confidence: 99%

Characterization of alkaline protease produced byStreptomyces griseorubensE44G and its possibility for controllingRhizoctoniaroot rot disease of corn

Al-Askar

Rashad

Hafez

et al. 2015

Biotechnology & Biotechnological Equipment

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The antifungal activity of Streptomyces griseorubens E44G against Rhizoctonia solani, the causal agent of root rot disease of corn, was investigated. The mycelial growth of R. solani was inhibited by S. griseorubens E44G, indicating that it has an antifungal potential. The antagonist, S. griseorubens E44G, was detected to have proteolytic activity, using the method of casein hydrolysis. Moreover, the protease production was optimized under submerged conditions. The purification and precipitation of protease were achieved by ammonium sulphate and Sephadex G-100 gel filtration chromatography. Protease activity was detected spectrophotometrically based on the production of tyrosine. The molecular weight of the enzyme (35 kDa) was determined by sodium dodecyl sulphate polyacrylamide gel electrophoresis . The optimum activity of the enzyme was detected at pH 8.5 and 60 C. The results indicated that the enzyme was thermostable and retained full activity even after 1 hour of incubation at 60 C. The purified enzyme substantially inhibited the growth of R. solani, indicating that this enzyme may be actually involved in the antagonistic process.

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OPTIMIZATION OF ALKALINE PROTEASE PRODUCTION BY <i>STREPTOMYCES AMBOFACIENS</i> IN FREE AND IMMOBILIZED FORM

Abstract: Optimization of alkaline protease production by Streptomyces ambofaciens NRRL 2420 in free and immobilized form was investigated using submerged fermentation technique. The optimum conditions for maximum alkaline protease production 342 unit mL

Cited by 20 publications

References 23 publications

Optimization of alkaline protease production by Streptomyces sp. strain isolated from saltpan environment

Optimization of alkaline protease production by Streptomyces sp. strain isolated from saltpan environment

Metabolic Responses of Bacterial Cells to Immobilization

Characterization of alkaline protease produced byStreptomyces griseorubensE44G and its possibility for controllingRhizoctoniaroot rot disease of corn

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