Optimization of an enzyme linked DNA aptamer assay for cardiac troponin I detection: synchronous multiple sample analysis on an integrated microfluidic platform

Abstract

In this study, an enzyme linked DNA aptamer based assay was optimized for human cardiac troponin I (cTnI) detection which is a prominent biomarker for acute myocardial infarction (AMI), on an integrated microfluidic platform.

Fluorescence Based on Surface Plasmon Coupled Emission for Ultrahigh Sensitivity Immunoassay of Cardiac Troponin I

“…For LOD estimation, we use linear regression of the data at small concentration close to zero, being considered a good approximation as seen in Figure 3 a–d. These levels of LODs presented are recognized as the lowest ever reported to date amid those obtained using optical technologies, such as label-free optical sensors and luminescence [ 10 , 19 , 22 , 41 , 42 , 43 , 44 , 45 ] as shown in Table 1 , where all LODs were reported using PBS without human serum.…”

“…Thus, the high sensitivity assay device and the related technologies have come into a key play for early diagnosis of urgent cases of cardiovascular diseases. Extensive efforts have been made in an attempt to enhance the sensitivity in detecting cTnI concentration using the assays including electrochemical method [ 15 , 16 ], transistor based assay [ 17 , 18 ], chemiluminescence [ 19 ], fluorescence immunoassay [ 20 ], colorimetric assay [ 21 ], surface plasmon resonance (SPR) [ 22 , 23 ], and optical microfiber based label-free assay [ 10 ], while point-of-care testing of cTnI was reported for test device portability without compromising the sensitivity [ 24 ].…”

Exploring Circulating Tumor Cells in Cholangiocarcinoma Using a Novel Glycosaminoglycan Probe on a Microfluidic Platform

“…The entire set up for operation of the chip was consisted of an air compressor (S‐301, Sharpgun Dental Instrument, Taiwan), a vacuum pump (UN‐90 V, Uni‐Crown, Taiwan), and a computer controlled EMV functional unit (SMC S070B‐SBG‐05; Wei‐Chia Electro Materials, Taiwan). [ 41 ] In a preprogrammed manner, the EMV functional unit automatically switched between the flow of compressed air and generation of vacuum. The operational conditions of the chip as displayed in Table S5 (Supporting Information) were determined after experimental determination of mixing and pumping characteristics (mentioned in the next section) of the chip and therefore unique for every chip.…”

Recent advances in cardiac biomarkers detection: From commercial devices to emerging technologies

“…Among them, acute myocardial infarction (AMI), acute coronary syndrome (ACS) and heart failure (HF) attract themost attention of scientists and clinicians [13]. Because of the very high frequency -one third of deaths [14,15] -of this pathology all over the world [2,7,8,12,[16][17][18][19][20][21], AMI is one of the WHO's major priorities [22]. Worldwide, between 5% and 20% of patients presenting to emergency departments (ED) do so with symptoms of myocardial infarction [23,24], which is defined as "cardiomyocyte necrosis in a clinical setting consistent with acute myocardial ischemia", according to the universal definition [25].…”