Optimization of Aqueous Extraction Conditions of Inulin from the Arctium lappa L. Roots Using Ultrasonic Irradiation Frequency

Esmaeili, Farzaneh; Hashemiravan, Mahnaz; Eshaghi, Mohammad Reza; Gandomi, Hassan

doi:10.1155/2021/5520996

Cited by 21 publications

(16 citation statements)

References 42 publications

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“…Teff flour (1.0 g) was taken in a 50 mL nylon centrifuge tube and soaked for 2 hours in 7 mL of 80:20 methanol-distilled water. The wetted sample was extracted under an ultrasonic water bath [ 27 ] for 30 min, 45 min, and 60 min at a fixed temperature (35°C). The mixture was centrifuged for 15 min at 3500 rpm, where the supernatant was decanted carefully into a second centrifuge tube.…”

Section: Methodsmentioning

confidence: 99%

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Polyphenol and flavonoid content in major Teff [Eragrostis tef (Zuccagni) Trotter] varieties in Amhara Region, Ethiopia

et al. 2022

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Teff [Eragrostis tef (Zuccagni) Trotter] is a small-sized cereal grain and an indigenous crop in Ethiopia. The Amhara region is one of the major teff producers regions in the country. However, information on the phenolic content of the region’s teff varieties is limited. Seventy-two teff samples were collected from three administrative zones (West Gojjam zone, Awi zone, and East Gojjam zone) of the Amhara region of Ethiopia. The samples’ total polyphenol and flavonoid contents were determined using colorimetric methods. The total flavonoid contents expressed as catechin equivalent, CE (i.e., under alkaline conditions) and quercetin equivalent, Q.E (i.e., under the methanolic solution of AlCl3) were found to be in the range of 7.66 ± 0.60–57.36 ± 3.87 mg C.E and 15.45 ± 0.15–113.12 ± 3.09 mg Q.E per 100 g of teff samples, respectively. The corresponding total polyphenol content (TPC), described as gallic acid equivalent (G.A.E.), was in the range of 46.21 ± 1.20–133.32 ± 5.44 mg G.A.E. The results showed that the mean TPC value of the teff samples from the West Gojjam zone was enriched with polyphenol than samples from the Awi zone and East Gojjam. Furthermore, it was noted that the mean TPC and TFC values did not vary significantly between samples of the East Gojjam and Awi zone (p > 0.05). In contrast, a significant difference in mean TPC and TFC-Q.E were noted between the sampling zone of East Gojjam and West Gojjam and between West Gojjam and Awi zones (p < 0.05). These significant variations in TPC and TFC might be due to observable variations in the agroecological zones and the genetic—make-up of the samples. Person correlation indicated a significant positive correlation matrix between the three variables (p = 0.01). The teff samples were trying to be classified based on their geographical origin using hierarchical cluster analysis (HCA) and biplots. Accordingly, the variance explained by component 1 (PC1) is 67.2%, and the variance explained by component 2 (PC2) is 20.0%.

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Section: Methodsmentioning

confidence: 99%

“…In this study, the catechin-like flavonoids were estimated as catechin equivalent following the method reported by Pekal and Pyrzynska [ 27 ] using AlCl 3 , NaNO 2, and NaOH solutions. Briefly, 2 mL of each catechin standard solution or sample solution was taken and treated with 0.6mL of 5%NaNO 2 .…”

Section: Methodsmentioning

confidence: 99%

Polyphenol and flavonoid content in major Teff [Eragrostis tef (Zuccagni) Trotter] varieties in Amhara Region, Ethiopia

et al. 2022

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“…Extractions were performed following the method of Adwan et al [1] and Esmaeili et al [22] with some modification using an ultrasonic bath, model DSA100-SK2 (Fuzhou Desen Precision Instruments Co., Ltd., Fujian, China). The UAE experiments were carried out at different ranges of solid-to-liquid ratios (1:15-1:25 g/mL), sonication temperatures (30-50 • C), and sonication times (30-60 min) following the designated experimental design.…”

Section: Plant Sample Extractionmentioning

confidence: 99%

“…The parameters employed were extraction time (30-60 min), extraction temperature (30-50 • C), and solid-liquid ratio (15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25). The extraction yield of gum was calculated using the final dry weight of extracted gum relative to the original gum powder [25].…”

Section: Experimental Designmentioning

confidence: 99%

Optimization of Ultrasound-Assisted Extraction of Bioactive Compounds from Acacia Seyal Gum Using Response Surface Methodology and Their Chemical Content Identification by Raman, FTIR, and GC-TOFMS

et al. 2021

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Acacia Seyal gum (ASG), also known as gum Arabic, is an antioxidant-rich soluble fiber. ASG has been reported to have many biological activities, including anticancer, antidiabetic, antiulcer, and immunomodulatory activity. Extraction of bioactive compounds from ASG is commonly performed using conventional extraction methods. However, these techniques have certain limitation in terms of extraction time, energy, and solvent requirements. Ultrasound-assisted extraction (UAE) could be used as an alternative technique to extract bioactive compounds in less time, at low temperature, and with less energy and solvent requirements. In this study, the UAE extraction of ASG was optimized using response surface methodology (RSM). A face-centered central composite design (FCCCD) was used to monitor the effect of different independent factors of ultrasound operation (sonication time, temperature, and solvent ratio) on ASG extraction yield. In addition, screening and characterization of phytochemicals in 60% ethanol ASG extract was carried out using Raman microscopy, Fourier transform infrared spectroscopy (FTIR), and gas chromatography time-of-flight mass spectroscopy (GC-TOFMS) analysis. The results indicated that, under optimal conditions (extraction time 45 min, extraction temperature 40 °C, and solid–liquid ratio of 1:25 g/mL), the yield of ASG was 75.87% ± 0.10. This yield was reasonably close to the predicted yield of 75.39% suggested by the design of experiment. The ANOVA revealed that the model was highly significant due to the low probability value (p < 0.0001). Raman spectrum fingerprint detected polysaccharides, such as galactose and glucose, and protein like lysine and proline, while FTIR spectrum revealed the presence of functional groups peaks value of alkanes, aldehydes, aliphatic amines, and phenol. GC-TOFMS spectroscopic detected the presence of strong d-galactopyranose, carotenoid, and lycopene antioxidant compounds. In conclusion, this study demonstrated that the UAE technique is an efficient method to achieve a high yield of ASG extracts. The selected model is adequate to optimize the extraction of several chemical compounds reported in this study.

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“…Moreover, extraction is very crucial for developing a rapid and efficient strategy. In this sense, ultrasound-assisted extraction (UAE) is considered as a promising approach to supersede the conventional methods because of the advantages of easy operation, high extraction efficiency, low solvent consumption, and short extraction times [ 26 , 27 ], which make it a quick and efficient approach for the pre-concentration of analytes from complex matrices. The efficiency of UAE is often influenced by the factors of extraction time, solvent concentration, solvent to solid ratio and ultrasonic power, so it further necessary to be optimized [ 28 , 29 ].…”

Section: Introductionmentioning

confidence: 99%

A Rapid and Efficient Strategy for Quality Control of Clinopodii herba Encompassing Optimized Ultrasound-Assisted Extraction Coupled with Sensitive Variable Wavelength Detection

et al. 2022

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Clinopodii herba is a folk herbal medicine for treatments of hemorrhagic disorders. However, there is not even a quantitative standard for clinopodii herba deposited in the Chinese Pharmacopoeia. The development of a strategy for rapid and efficient extraction and simultaneous detection of multiple components in clinopodii herba is therefore of great value for its quality evaluation. Here, a variable wavelength strategy was firstly applied to quantity multiple components by segmental monitoring by UHPLC with diode array detector following ultrasound-assisted extraction. The parameters of ultrasound-assisted extraction were optimized using single factor optimization experiments and response surface methodology by a Box–Behnken design combined with overall desirability. Subsequently, a rapid, efficient, and sensitive method was applied for simultaneous determination of eleven compounds, which represented the major and main types of components in clinopodii herba. Moreover, the performance of the validated method was successfully applied for the quality control of various batches of clinopodii herba and provided sufficient supporting data for the optimum harvest time. The Box-Behnken-optimized ultrasound-assisted extraction coupled with variable wavelength detection strategy established in this work not only improves the quality control of clinopodii herba, but also serves as a powerful approach that can be extended to quality evaluation of other traditional Chinese medicines.

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Optimization of Aqueous Extraction Conditions of Inulin from the Arctium lappa L. Roots Using Ultrasonic Irradiation Frequency

Cited by 21 publications

References 42 publications

Polyphenol and flavonoid content in major Teff [Eragrostis tef (Zuccagni) Trotter] varieties in Amhara Region, Ethiopia

Polyphenol and flavonoid content in major Teff [Eragrostis tef (Zuccagni) Trotter] varieties in Amhara Region, Ethiopia

Optimization of Ultrasound-Assisted Extraction of Bioactive Compounds from Acacia Seyal Gum Using Response Surface Methodology and Their Chemical Content Identification by Raman, FTIR, and GC-TOFMS

A Rapid and Efficient Strategy for Quality Control of Clinopodii herba Encompassing Optimized Ultrasound-Assisted Extraction Coupled with Sensitive Variable Wavelength Detection

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