Optimization of aqueous two-phase partitioning for purification of recombinant Eupenicillium terrenum fructosyl peptide oxidase

Shahbazmohammadi, Hamid; Sardari, Soroush; Omidinia, Eskandar

doi:10.1016/j.bcab.2019.101344

Cited by 3 publications

(2 citation statements)

References 24 publications

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“…Since the interactions among different constituents in culture media are complex, it is bene cial to screen those using statistical models. Statistical experimental designs including Plackett-Burman design (PBD) and response surface methodology (RSM) are useful strategies to optimize conditions, and achieve favorable response in an experiment (Shahbazmohammadi et al, 2019;Papaneophytou et al, 2013;Ghoshoon et al, 2015). RSM is a complex of statistical methods for evaluating relation between experiment response, and a set of variables.…”

Section: Introductionmentioning

confidence: 99%

Lactose inducible fermentation in Escherichia coli for improved production of recombinant urate oxidase: Optimization by statistical experimental designs

Najjari

Shahbazmohammadi

Omidinia

2021

Biocatalysis and Agricultural Biotechnology

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The enzyme urate oxidase (UOX) is used as a drug for preventing and treatment of chemotherapyinduced hyperuricemia. This study deals with the statistical optimization of lactose inducible fermentation for production of soluble recombinant Aspergillus avus UOX. 10 variables were investigated by Plackett-Burman design (PBD), and the most signi cant factors were further optimized by central composite design (CCD). PBD results indicated that glycerol, yeast extract, tryptone, and lactose affected UOX activity signi cantly. The CCD results showed that the maximum enzyme activity (19.34 U/ml) could be achieved under the optimum conditions of glycerol 0.87 g/L, yeast extract 9.11 g/L, tryptone 10.29 g/L, K 2 HPO 4 1.81 g/L, and lactose 12.79 g/L. When the same induction strategy was tested at shake ask, 19.34 U/mL of UOX activity was obtained, which was 12.5 folds higher than IPTG induction protocol. Furthermore, the lower total cost (0.7 vs. 13.5 €) was additionally feature that con rmed the suitability of the lactose induction method. Collectively, our results showed that design of experiment methodology can be applied as a suitable tool for improved production of UOX using lactose as the inducer.

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Section: Introductionmentioning

confidence: 99%

Lactose inducible fermentation in Escherichia coli for improved production of recombinant urate oxidase: Optimization by statistical experimental designs

Najjari

Shahbazmohammadi

Omidinia

2021

Biocatalysis and Agricultural Biotechnology

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“…(BORGES, et al, 2016;YAZDABADI et al, 2019). Além disso, uma vez que ambas as fases são compostas de água, esses sistemas têm um efeito estabilizador na estrutura proteica, fornecendo um ambiente não desnaturador para materiais biológicos, evitando perda das atividades biológicas (SANTHI et al, 2020;SHAHBAZMOHAMMADI et al, 2019).…”

Section: Revisão Bibliográficaunclassified

Equilíbrio líquido-líquido e partição de albumina de soro bovino e lisozima em sistemas aquosos bifásicos envolvendo PEG e sais

Oliveira¹

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DedicatóriaA Deus, aos meus pais, Elio e Islene (in memorian), ao meu esposo, Anderson e ao meu filho, Thiago. AgradecimentosA Deus, por direcionar todos os meus caminhos.Aos meus pais, Elio Cândido de Oliveira e Islene Aparecida de Oliveira (in memorian), pelo alicerce ao longo de toda a minha vida.Ao meu querido esposo, Anderson Lopes Bonfim, pelo apoio emocional, financeiro e pela paciência durante a confecção desta tese.Ao meu amado filho, Thiago de Oliveira Lopes, que mesmo tão pequenino foi muito importante nesta etapa da minha vida.Aos meus queridos irmãos, Wyner, Alessandra, Aretha por sempre torcerem pela minha vitória.Aos meus sobrinhos, Carlos Eduardo, Lucas Gabriel e Gabriel Henrique por proporcionarem inúmeros momentos de alegria.Ao meu eterno orientador, Prof. Dr. Martin Aznar (in memorian), pelos ensinamentos, carisma e exemplo de profissional dedicado.A minha orientadora, Profa. Dra. Roberta Ceriani, pela extrema paciência e pelos valiosos ensinamentos.Ao meu coorientador, Prof. Dr. Elias de Souza Monteiro Filho, pelo empenho em colaborar com este trabalho.À UESC (Universidade Estadual de Santa Cruz) por me proporcionar um período de docência que foi importantíssimo para o meu crescimento profissional.

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A comprehensive review on fructosyl peptide oxidase as an important enzyme for present hemoglobin A1c assays

Rahmatabadi,

Bashiri,

Soleymani

2024

Biotech and App Biochem

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Glycated proteins are generated by binding of glucose to the proteins in blood stream through a nonenzymatic reaction. Hemoglobin A1c (HbA1c) is a glycated protein with glucose at the N‐terminal of β‐chain. HbA1c is extensively used as an indicator for assessing the blood glucose concentration in diabetes patients. There are different conventional clinical methods for the detection of HbA1c. However, enzymatic detection method has newly obtained great attention for its high precision and cost‐effectiveness. Today, fructosyl peptide oxidase (FPOX) plays a key role in the enzymatic measurement of HbA1c, and different companies have marketed HbA1c assay systems based on FPOX. Recent investigations show that FPOX could be used in assaying HbA1 without requiring HbA1c primary digestion. It could also be applied as a biosensor for HbA1c detection. In this review, we have discussed the recent improvements of FPOX properties, different methods of FPOX purification, solubility, and immobilization, and also the use of FPOX in HbA1c biosensors.

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Optimization of aqueous two-phase partitioning for purification of recombinant Eupenicillium terrenum fructosyl peptide oxidase

Cited by 3 publications

References 24 publications

Lactose inducible fermentation in Escherichia coli for improved production of recombinant urate oxidase: Optimization by statistical experimental designs

Lactose inducible fermentation in Escherichia coli for improved production of recombinant urate oxidase: Optimization by statistical experimental designs

Equilíbrio líquido-líquido e partição de albumina de soro bovino e lisozima em sistemas aquosos bifásicos envolvendo PEG e sais

A comprehensive review on fructosyl peptide oxidase as an important enzyme for present hemoglobin A1c assays

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