Optimization of Bacillus subtilis cell growth effecting jiean-peptide production in fed batch fermentation using central composite design

Zhong, Juan; Xiao-yong, Zhang; Ren, Yiwei; Yang, Jie; Hong, Tao; Zhou, Jinyan

doi:10.1016/j.ejbt.2014.04.010

Cited by 25 publications

(10 citation statements)

References 28 publications

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“…A total of 20 experimental runs were conducted and a general form of second-order polynomial quadratic model was used to describe the influence of independent variables on the response as described in Eq. (2) (Hay et al, 2012;Zhong et al, 2014):…”

Section: Experimental Designmentioning

confidence: 99%

Photocatalytic degradation of industrial pulp and paper mill effluent using synthesized magnetic Fe 2 O 3 -TiO 2 : Treatment efficiency and characterizations of reused photocatalyst

Subramonian

Chai

2017

Journal of Environmental Management

119

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In this work, heterogeneous photocatalysis was used to treat pulp and paper mill effluent (PPME). Magnetically retrievable FeO-TiO was fabricated by employing a solvent-free mechanochemical process under ambient conditions. Findings elucidated the successful incorporation of FeO into the TiO lattice. FeO-TiO was found to be an irregular and slightly agglomerated surface morphology. In comparison to commercial P25, FeO-TiO exhibited higher ferromagnetism and better catalyst properties with improvements in surface area (58.40 m/g), pore volume (0.29 cm/g), pore size (18.52 nm), and band gap (2.95 eV). Besides, reusability study revealed that FeO-TiO was chemically stable and could be reused successively (five cycles) without significant changes in its photoactivity and intrinsic properties. Additionally, this study demonstrated the potential recovery of FeO-TiO from an aqueous suspension by using an applied magnetic field or sedimentation. Interactive effects of photocatalytic conditions (initial effluent pH, FeO-TiO dosage, and air flow-rate), reaction mechanism, and the presence of chemical oxidants (HO, BrO, and HOCl) during the treatment process of PPME were also investigated. Under optimal conditions (initial effluent pH = 3.88, [FeO-TiO] = 1.3 g/L, and air flow-rate = 2.28 L/min), the treatment efficiency of FeO-TiO was 98.5% higher than the P25. Based on Langmuir-Hinshelwood kinetic model, apparent rate constants of FeO-TiO and P25 were 9.2 × 10 and 2.7 × 10 min, respectively. The present study revealed not only the potential of using magnetic FeO-TiO in PPME treatment but also demonstrated high reusability and easy separation of FeO-TiO from the wastewater.

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Section: Experimental Designmentioning

confidence: 99%

Photocatalytic degradation of industrial pulp and paper mill effluent using synthesized magnetic Fe 2 O 3 -TiO 2 : Treatment efficiency and characterizations of reused photocatalyst

Subramonian

Chai

2017

Journal of Environmental Management

119

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“…Glucose supplementation on batch fermentation of B. subtilis obtained higher cell dry weight, with optimum glucose concentration identified as 3.07% (w/v) (Zhong et al 2014). According to the results in this experiment, excess carbon source in modified media did not enhance biomass yield and xylanase expressed from recombinant B. subtilis was not higher than in original SOC media with 20 mM glucose.…”

Section: Discussionmentioning

confidence: 55%

The utilization of auto-inducible Plyb promoter and media optimation for cell density-dependent expression of recombinant xylanase in Bacillus subtilis DB104

Haniyya¹,

Achnafani²,

Ulfah³

et al. 2020

Microbiol indones

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Strong promoters are one of the fundamental aspects to increase the level of gene expression, and one of approach to improve the recombinant enzyme productivity so that the efficiency of production cost for enzyme production in industrial scale can be reached. Here we assessed the application of a cell density-dependent promoter and media optimation to promote cell growth and protein expression of Bacillus subtilis without excess usage of inducers. An auto-inducible Pylb promoter that is potential to provide inducer-free enzyme production was cloned and introduced into xylanase recombinant system in B. subtilis DB104 by PCR cloning and protoplast transformation. A 200 bp target gene was successfully inserted in between xynCM1 ORF -coding for B. halodurans CM1 xylanase- and its native promoter sequence at the upstream region. The disruption of the native promoter was intended to replace the native promoter with Pylb. Recombinant xylanase gene under Pylb was successfully expressed in B. subtilis DB104 and the enzyme was produced at stationary phase. Different media with various concentrations of glucose and nitrogen were used to optimize recombinant xylanase expression. It achieved a higher level of xylanase expression compared to wild-type and recombinant xylanase with native promoter B. subtilis in media containing a 2-fold recipe of LB media thus leads to increase cell density and xylanase expression (81.461 U mL-1).

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“…They used CCD and RSM to optimize the three extraction variables (temperature, pH, and process time) and reported RSM as an efficient tool for the extraction of 3-DMC from the fermentation medium (Dubey et al, 2011). Similary, a group of researchers used RSM in fed batch fermentation condition to improve the production of jiean-peptide (JAA) from Bacillus subtilis and found 44% enhanced yield of JAA in comparison to the production under batch fermentation (Zhong et al, 2014). Likewise, Ghasemi et al (2011) used RSM technique for improving the extraction performance of various components of essential oils, such as α-pinene (31.8%), 1,8-cineole (24.6%), limonene (14.8%), linalool (8.3%), and α-terpinolene (4.8%), present in the leaves of Myrtus communis.…”

Section: Media Optimization Strategiesmentioning

confidence: 99%

Strategies for Fermentation Medium Optimization: An In-Depth Review

et al. 2017

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Optimization of production medium is required to maximize the metabolite yield. This can be achieved by using a wide range of techniques from classical “one-factor-at-a-time” to modern statistical and mathematical techniques, viz. artificial neural network (ANN), genetic algorithm (GA) etc. Every technique comes with its own advantages and disadvantages, and despite drawbacks some techniques are applied to obtain best results. Use of various optimization techniques in combination also provides the desirable results. In this article an attempt has been made to review the currently used media optimization techniques applied during fermentation process of metabolite production. Comparative analysis of the merits and demerits of various conventional as well as modern optimization techniques have been done and logical selection basis for the designing of fermentation medium has been given in the present review. Overall, this review will provide the rationale for the selection of suitable optimization technique for media designing employed during the fermentation process of metabolite production.

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Optimization of Bacillus subtilis cell growth effecting jiean-peptide production in fed batch fermentation using central composite design

Cited by 25 publications

References 28 publications

Photocatalytic degradation of industrial pulp and paper mill effluent using synthesized magnetic Fe 2 O 3 -TiO 2 : Treatment efficiency and characterizations of reused photocatalyst

Photocatalytic degradation of industrial pulp and paper mill effluent using synthesized magnetic Fe 2 O 3 -TiO 2 : Treatment efficiency and characterizations of reused photocatalyst

The utilization of auto-inducible Plyb promoter and media optimation for cell density-dependent expression of recombinant xylanase in Bacillus subtilis DB104

Strategies for Fermentation Medium Optimization: An In-Depth Review

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