Optimization of conditions for flow-through partial-filling affinity capillary electrophoresis to estimate binding constants of ligands to receptors

Brown, Arianna; Desharnais, Robert A.; Roy, B. C.; Malik, Sanku; Gomez, Frank A.

doi:10.1016/j.aca.2005.03.036

Cited by 19 publications

(14 citation statements)

References 37 publications

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“…Other new modifications of PF-CAE involve flowthrough PF-CAE, competitive flow-through PF-CAE, and multiple-step ligand injection PF-CAE [129,130]. Optimization of conditions for flow-through PF-CAE, including the determination of minimal injection time of ligand required to estimate binding constants of dipeptide D-Ala-D-Ala ligand to glycopeptide antibiotic, vancomycin, has been recently reported [131]. Binding constants between macrocyclic antibiotics receptors and peptide ligands can be determined also by dual-marker form of CAE [132].…”

Section: Affinity Electrophoresismentioning

confidence: 98%

Recent developments in capillary electrophoresis and capillary electrochromatography of peptides

2006

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The article gives a comprehensive review on the recent developments in the applications of high-performance capillary electromigration methods, zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography, to analysis, preparation, and physicochemical characterization of peptides. The article presents new approaches to the theoretical description and experimental verification of electromigration behavior of peptides, covers the methodological aspects of capillary electroseparations of peptides, such as rational selection of separation conditions, sample preparation, suppression of peptide adsorption, new developments in individual separation modes, and new designs of detection systems. Several types of applications of capillary electromigration methods to peptide analysis are presented: conventional qualitative and quantitative analysis, purity control, determination in biomatrices, monitoring of chemical and enzymatical reactions and physical changes, amino acid and sequence analysis, and peptide mapping of proteins. Some examples of micropreparative peptide separations are given and capabilities of capillary electromigration techniques to provide important physicochemical characteristics of peptides are demonstrated.

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Section: Affinity Electrophoresismentioning

confidence: 98%

Recent developments in capillary electrophoresis and capillary electrochromatography of peptides

2006

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“…Methodologically new approach for estimation of binding constants of peptide ligands (N-acetyl and N-succinyl derivatives of a dipeptide D-Ala-D-Ala) to glycopeptide antibiotic receptor (vancomycin from Streptomyces orientalis), flowthrough partial-filling CAE (FTPF-CAE), has been developed by Gomez and coworkers [121]. In this technique, the capillary is first partially filled with a zone of ligand followed by a sample plug containing receptor and noninteracting standards.…”

Section: Affinity Electrophoresismentioning

confidence: 99%

Recent developments in CE and CEC of peptides

Kašička

2007

Electrophoresis

118

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The article brings a comprehensive survey of recent developments and applications of high-performance capillary electromigration methods, zone electrophoresis, ITP, IEF, affinity electrophoresis, EKC, and electrochromatography, to analysis, preparation, and physicochemical characterization of peptides. New approaches to the theoretical description and experimental verification of electromigration behavior of peptides and to methodology of their separations, such as sample preparation, adsorption suppression, and detection, are presented. Novel developments in individual CE and CEC modes are shown and several types of their applications to peptide analysis are presented: conventional qualitative and quantitative analysis, purity control, determination in biomatrices, monitoring of chemical and enzymatical reactions and physical changes, amino acid and sequence analysis, and peptide mapping of proteins. Some examples of micropreparative peptide separations are given and capabilities of CE and CEC techniques to provide important physicochemical characteristics of peptides are demonstrated.

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“…Since the first papers in 1992 [3][4][5][6] documenting the use of ACE to measure affinity parameters between biological species, its use in probing a variety of receptor-ligand interactions has expanded to include protein-ligand [7][8][9][10][11][12][13][14][15], peptide-metal [16,17], peptide-peptide [18][19][20][21][22][23][24][25][26][27], protein-peptide [28], protein-antibody [29], polymer-peptide [30], antibodyantigen [31], enzyme-drug [32], and polymer-CD [33]. ACE affords a number of advantages over other techniques to study biological interactions.…”

Section: Introductionmentioning

confidence: 99%

Implementation of chemometric methodology in ACE: Predictive investigation of protein–ligand binding

et al. 2007

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An ACE predictive investigation of protein-ligand binding using a highly effective chemometric response surface design technique is presented. Here, K(d) was estimated using one noninteracting standard which relates to changes in the electrophoretic mobility of carbonic anhydrase B (CAB, EC 4.2.1.1) on complexation with the ligand 4-carboxybenzenesulfonamide (CBSA) present in the electrophoresis buffer. Experimental factors including injection time, capillary length, and applied voltage were selected and tested at three levels in a Box-Behnken design. Statistical analysis results were used to create a mathematical model for response surface prediction via contour and surface plots at a given target response (K(d) = 1.19x10(-6) M). As expected, there were a number of predicted solutions that reached our target response based on the significance of each factor at appropriate levels. The adequacy of the model was validated by experimental runs with the predicted model solution (capillary length = 47 cm, voltage = 11 kV, injection time = 0.01 min) presented in detail as an example.

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Optimization of conditions for flow-through partial-filling affinity capillary electrophoresis to estimate binding constants of ligands to receptors

Cited by 19 publications

References 37 publications

Recent developments in capillary electrophoresis and capillary electrochromatography of peptides

Recent developments in capillary electrophoresis and capillary electrochromatography of peptides

Recent developments in CE and CEC of peptides

Implementation of chemometric methodology in ACE: Predictive investigation of protein–ligand binding

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