Optimization of cultivation conditions for extracellular polysaccharide and mycelium biomass by Morchella esculenta As51620

Xu, Huaxi; Sun, Luyang; Shi, Yanjun; Yahua, Wu; Zhang, Bin; Zhao, Daqing

doi:10.1016/j.bej.2007.08.013

Cited by 73 publications

(44 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The limitation of such method can be avoided by using statistical experimental design, a powerful and useful tool, which can allow explaining interactions between the different variables and decreasing the process variability (Frey et al 2003). The most popular choices are the Plackett-Burman design (PBD) and the central composite design (CCD), along with the response surface analysis (Liu et al 2005;Xu et al 2008). The response surface methodology (RSM) is a kind of statistical technique for designing experiments, evaluating the relative significance of several independent variables, and determining the optimum conditions for desirable responses (Anderson-Cook et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Statistical physical and nutrient optimization of bioplastic polyhydroxybutyrate production by Cupriavidus necator

Aramvash

Shahabi

Aghjeh

et al. 2015

Int. J. Environ. Sci. Technol.

View full text Add to dashboard Cite

Polyhydroxyalkanoates are biodegradable polymer materials that accumulate in numerous bacteria. The polyhydroxybutyrate is the most common type of polyhydroxyalkanoates, which potentially serves as precursor for bioplastic production. The most extensively studied polyhydroxybutyrate producing bacteria is Cupriavidus necator due to its capability to accumulate large amounts of this biopolymer in simple culture medium. Accumulation of polyhydroxyalkanoates granules in the cytoplasm of C. necator significantly depended on pH, aeration, carbon sources, nitrogen sources, and minerals in the culture medium. In the present study, the effect of both nutritional and physical variables on polyhydroxybutyrate production was investigated in order to optimize these conditions. At first, on the basis of one-factor-at-a-time experiments, fructose and ammonium chloride were found to be the most suitable sources of carbon and nitrogen for biopolymer production. Then the most significant factors affecting granules accumulation were recognized as fructose, agitation speed, KH 2 PO 4 , and initial pH using the Plackett-Burman and central composite design. ANOVA analysis showed significant interaction between fructose and agitation speed. After optimization of the medium, compositions for polyhydroxybutyrate production were determined as follows: fructose 35 g/L, KH 2 PO 4 1.75 g/L, MgSO 4 Á7H 2 O 1.2 g/L, citric acid 1.7 g/L, trace element 10 mL/L, initial pH = 7, and agitation speed 175 rpm. Under this optimal culture conditions, the maximum yield of PHB was 7.48 g/L. The present strategies included in this study could be used for PHB production by this bacterium. These results are the highest values of PHB ever obtained from batch culture of C. necator reported so far.

show abstract

Section: Introductionmentioning

confidence: 99%

Statistical physical and nutrient optimization of bioplastic polyhydroxybutyrate production by Cupriavidus necator

Aramvash

Shahabi

Aghjeh

et al. 2015

Int. J. Environ. Sci. Technol.

View full text Add to dashboard Cite

show abstract

“…5 to 7. The shape of contour reflects the strength of the interaction between two factors, the oval indicates strong interaction and the circle indicates weak interaction between two factors (Xu et al, 2008). Fig.…”

Section: Response Surface Optimization Resultsmentioning

confidence: 99%

Optimization of Enzymatic Hydrolysis of Freeze-Dried Sea Cucumber Powder with Response Surface Methodology and Its Antioxidant Evaluation

Pang¹,

Sun²,

Liu³

2016

American Journal of Biochemistry and Biotechnology

View full text Add to dashboard Cite

In this study, freeze-dried sea cucumber powder was used as raw material and the preparation processing conditions were studied for hydrolysis of sea cucumber powder with neutral protease using Response Surface Methodology (RSM) to obtain a high concentration of sea cucumber polypeptide. The effects of hydrolysis temperature, pH, enzyme dosage and hydrolysis time on the degree of hydrolysis (DH) were studied. Based on the single factor experiment, Box-Benhnken center composite experiment was carried out with three factors and three levels, including hydrolysis temperature, pH and enzyme dosage. RSM was used to determine the effect of prime factors on the DH and establish a regression model. The results showed that the optimal hydrolysis conditions were as follows: enzyme dosage of 5753 U g −1 dry matter, hydrolysis temperature of 52°C, pH of 8.3, substrate concentration of 5% and hydrolysis time of 5 h. Under the optimal conditions, the DH was up to 16.01%, which matched well with the predicted value. With VC as the positive control, the scavenging ability of the hydrolyzate on DPPH radicals, hydroxyl radicals and superoxide radicals were very powerful, which mean that the hydrolyzate of sea cucumber powder has good antioxidant activity.

show abstract

“…This technique cannot determine the exact quantity but can provide indication and tendency regarding the necessity of each factor in relatively few experiments. Xu et al (2008) optimized the production conditions of extracellular polysaccharide and mycelia biomass by Morchella esculenta As51620 using PB design first and then RSM. There has been no report so far on optimizing the process parameters for producing EPS and mycelia biomass by S. rugosoannulata in submerged cultivation.…”

Section: Introductionmentioning

confidence: 99%

Statistical optimization of cultivation conditions for exopolysacchride production and mycelia growth by Stropharia rugosoannulata

et al. 2010

View full text Add to dashboard Cite

Response surface methodology (RSM) was successfully applied to investigate the effect of cultivation temperature, time and volume of media on mycelia growth, in the light of exopolysaccharide content (EPC) and dry cell weight (DCW), by the fungus Stropharia rugosoannulata in submerged culture. The three significant factors influencing EPC and DCW were based on the results of a previous Plackett-Burman (PB) design. Results showed that the maximum predicted values of EPC and DCW reached 1,498.17 mg l −1 and 9.89 g l −1 , while the optimized conditions of cultivation temperature, time and volume of media for EPC and DCW were 28.35°C, 6.67 days, and 110.29 ml, and 28.23°C, 6.58 days, and 109.17 ml, respectively. When the above conditions were 28.29°C, 6.63 days, and 109.69 ml, the simultaneously maximal yield of exopolysaccharide and mycelia biomass were 1,498.08 mg l −1 and 9.88 g l −1 , respectively. These predicted values were also verified by validation experiments.

show abstract

Optimization of cultivation conditions for extracellular polysaccharide and mycelium biomass by Morchella esculenta As51620

Cited by 73 publications

References 14 publications

Statistical physical and nutrient optimization of bioplastic polyhydroxybutyrate production by Cupriavidus necator

Statistical physical and nutrient optimization of bioplastic polyhydroxybutyrate production by Cupriavidus necator

Optimization of Enzymatic Hydrolysis of Freeze-Dried Sea Cucumber Powder with Response Surface Methodology and Its Antioxidant Evaluation

Statistical optimization of cultivation conditions for exopolysacchride production and mycelia growth by Stropharia rugosoannulata

Contact Info

Product

Resources

About