2024
DOI: 10.1038/s41598-024-77880-y
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Optimization of curdlan production and ultrasound assisted extraction processes from Priestia megaterium

Natasha Aquinas,
Bhat M. Ramananda,
Subbalaxmi Selvaraj

Abstract: In this study, the upstream and downstream production processes of curdlan from Priestia megaterium were optimized to enhance its yield. Additionally, a novel extraction method was developed for curdlan recovery. Optimization studies were conducted using Central composite design (CCD). Curdlan yield improved from 0.15 g/L (unoptimized) to 0.46 g/L (3-fold increase) when fermentation was carried out in CCD-optimized media of (w/v) sucrose 20%, urea 0.1%, KH2PO4 0.02%, agitation speed 250 rpm. To further enhance… Show more

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