Optimization of DNA extraction in the spiny spider crab Maja brachydactyla: determining the best extraction method, sample conservation and starting tissue

Rodríguez-Pena, Elba; Verísimo, Patricia; Fernández, Luis; González‐Tizón, Ana M.; Martı́nez-Lage, Andrés

doi:10.1007/s12686-017-0685-5

Cited by 2 publications

(1 citation statement)

References 9 publications

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“…While COI was analysed in all the samples, 16S was only sequenced for the 11 ovigerous females from Golfo Á rtabro and their eggs. The PCR conditions for the amplification of 16S rDNA were described in Rodríguez-Pena et al [59]. COI fragment was amplified using the primers COIMaja_F 5´-gaatggccggaacatcttta-3´, and COIMaja_R 5´-ccaccagctggatcaaagaa-3´and the NZYTaq 2xGreen Master Mix separate MgCl 2 kit (NZYTech) according to the man-ufacturer´s protocol in a final concentration of 1.5 mM of MgCl 2 and 0.5μM of each primer.…”

Section: Methodsmentioning

confidence: 99%

High incidence of heteroplasmy in the mtDNA of a natural population of the spider crab Maja brachydactyla

et al. 2020

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Mitochondria are mostly inherited by maternal via, that is, only mitochondria from eggs are retained in the embryos. However, this general assumption of uniparentally transmitted, homoplasmic and non-recombining mitochondrial genomes is becoming more and more controversial. The presence of different sequences of mtDNA within a cell or individual, known as heteroplasmy, is increasingly reported in several taxon of animals, such as molluscs, arthropods and vertebrates. In this work, a considerable frequency of heteroplasmy were detected in the COI and 16S genes of the spider crab Maja brachydactyla, possibly associated to hybridisation with the congeneric species Maja squinado. This finding is a fact to keep in mind before addressing molecular analyses based on mitochondrial markers, since the assumption of maternal inheritance could lead to erroneous results. As M. brachydactyla is a commercial species, heteroplasmy is an important aspect to take into account for the fisheries management of this resource, since effective population size could be overestimated. OPEN ACCESSCitation: Rodríguez-Pena E, Verísimo P, Fernández L, González-Tizón A, Bárcena C, Martínez-Lage A (2020) High incidence of heteroplasmy in the mtDNA of a natural population of the spider crab Maja brachydactyla. PLoS ONE 15(3): e0230243.

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Section: Methodsmentioning

confidence: 99%

High incidence of heteroplasmy in the mtDNA of a natural population of the spider crab Maja brachydactyla

et al. 2020

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Differences in prevalence of multiple paternity in the spiny spider crabMaja brachydactylain two localities that differ in female fecundity, fishing intensity, and management measures

Rodríguez-Pena

Verísimo

Tully

et al. 2021

ICES Journal of Marine Science

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Many marine species are under high fishing pressure, which can cause alterations in their mating systems and the structure of their populations. The spiny spider crab Maja brachydactyla is a commercial decapod on the east Atlantic coast. In Galicia, the most exploited region in Spain, the landing of ovigerous females is prohibited, favouring exploitation focused on males. The removal of males, especially the largest ones, may lead to sperm limitation and a reduction in the average size over generations. In these cases, polyandry can work as a mechanism to protect females against sperm limitation and to increase genetic diversity and effective population size. This study analyses the multiple paternity in M. brachydactyla in two localities that differ in female fecundity, fishing intensity, and management measures. Our results showed multiple paternity in this species for the first time, with a moderate bias between sires. However, the frequency of multiple paternity was almost seven times higher in the intensely exploited Golfo Ártabro (NW Spain; 69%) than in Carna (W Ireland; 10%), where exploitation levels and female fecundity are lower.

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Optimization of DNA extraction in the spiny spider crab Maja brachydactyla: determining the best extraction method, sample conservation and starting tissue

Cited by 2 publications

References 9 publications

High incidence of heteroplasmy in the mtDNA of a natural population of the spider crab Maja brachydactyla

High incidence of heteroplasmy in the mtDNA of a natural population of the spider crab Maja brachydactyla

Differences in prevalence of multiple paternity in the spiny spider crabMaja brachydactylain two localities that differ in female fecundity, fishing intensity, and management measures

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