Optimization of essential oil yield from Vietnamese green pepper (Piper nigrum) using hydro-distillation method

Dao, Tan Phat; Tran, T. H.; Nhan, Nguyen Phu Thuong; Quyen, Ngo Thi Cam; Tien, L X; Anh, Trieu Tuan; Quân, Phạm Minh; Nguyen, Ngoc Hoi; Anh, L L T; Linh, Huynh Thi Kieu

doi:10.1088/1757-899x/736/2/022039

Cited by 14 publications

(6 citation statements)

References 35 publications

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“…To collect the essential oil, hydro-distillation was performed over 3 h using hexane as a solvent. 14 The formula and components of the oregano essential oil were determined by gas chromatography/mass spectrometry 15 (Agilent GC System 7890A, Agilent Technologies Inc., USA). The EM yield was 0.5%.…”

Section: Methodsmentioning

confidence: 99%

Formulation of an Origanum vulgare based dental gel with antimicrobial activity

Badekova

Атажанова

Kačergius

et al. 2021

Journal of Taibah University Medical Sciences

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Objectives This study aims to formulate a new Origanum vulgare anti-caries dental gel with high antimicrobial activity. Methods O. vulgare essential oil was extracted using hydro-distillation, ethanol extraction, gas chromatography/mass spectrometry, and high-performance liquid chromatography methods. Antimicrobial activity of the produced gels with oregano oil and extract was determined through the disco-diffusion method. The effectiveness of O. vulgare essential oil was tested in vitro for Streptococcus mutans biofilm using colorimetric analysis. Results O. vulgare essential oil inhibited the growth of S. mutans biofilm by 98% compared with unexposed control bacteria ( p <0.05 ). Five samples of anti-caries gel (ACDG1, ACDG2, ACDG3, ACDG4, ACDG5) were formulated using the obtained oregano essential oil and extract. Based on the microbiological study results, the ACDG1 and ACDG3 gel samples exhibited high antimicrobial activity against the gram-positive bacterial strains of Staphylococcus aureus and Bacillus subtilis and the yeast fungus Candida albicans and moderate antimicrobial activity against the gram-negative strains of Escherichia coli and Pseudomonas aeruginosa . Conclusion Based on the results of this study, the ACDG3 sample may be considered an anti-caries gel owing to its high antimicrobial activity. This sample has good organoleptic properties compared to other samples, produces relatively high antimicrobial activity, and guards against cariogenic biofilms of S. mutans.

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Section: Methodsmentioning

confidence: 99%

Formulation of an Origanum vulgare based dental gel with antimicrobial activity

Badekova

Атажанова

Kačergius

et al. 2021

Journal of Taibah University Medical Sciences

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“…The MP-EO was prepared by water distillation (hydro-distillation method) [15] using a Clevenger apparatus. A total of 100 gr of the plant leaves and flowers were completely powdered and mixed in a balloon with 500 ml of water and then connected to a Clevenger device.…”

Section: Preparation Of Mp-eomentioning

confidence: 99%

Effects of Ionizing Radiation on Human Peripheral Blood Mononuclear Cells (PBMCs) in the Presence of Mentha-Pulegium Essential Oil: A Study on the Radioprotective Effect

Hamzian

2022

J Biomed Phys Eng

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Background:The Mentha-Pulegium essential oil (MP-EO) contains different antioxidant compounds and reduces the indirect effects of dispersed ionizing radiation on biological systems.Objective: The current study aimed to assess a possible radio-protective effect of MP-EO on peripheral blood mononuclear cells (PBMCs). Material and Methods:In this experimental study, MP-EO was firstly prepared and PBMCs were then irradiated in various groups with doses of 25 and 200 cGy of X-rays in the presence of IC10 of MP-EO. After incubation times of 48h and 72h, the survival, apoptosis, and necrosis percentages of PBMCs were determined by MTT assay and flow cytometry analyses; the radio-protective effect of MP-EO was examined.Results: In the presence of 80 µg/ml (IC10) MP-EO, the mean survival percentage of irradiated PBMCs by radiation doses of 25 and 200 cGy was significantly increased after 48h of incubation compared with the control. At 72h of incubation, the mean survival percentage of irradiated PBMCs was significantly increased only at 25 cGy. The percentage of apoptosis and necrosis of PBMCs was significantly reduced in the presence of the MP-EO at both incubation times and radiation doses; therefore, the highest reduction was at 200 cGy and 48h incubation compared to the control. Conclusion:MP-EO as a natural, non-toxic, and cost-effective compound can exhibit a favorable in-vitro radio-protective effect by increasing the survival and decreasing the percentage of apoptosis and necrosis of irradiated PBMCs.

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“…The right size for the extraction process is to grind the iced skin for 1 minute to examine other factors. The selection of material size in this study was referenced to previous studies [19,20] Fig. 3.…”

Section: Investigation Of Materials Sizementioning

confidence: 99%

Research methods of refined oil from peel calamondin (Citrus microcarpa) from Tien Giang Province, Vietnam

2021

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The research on extraction and refining of oil was conducted on the calamondin peels purchased in Thu Duc Market. Them originated from the province of Tien Giang in Viet Nam. The purpose of research evaluation of the quality of refined oil by refining methods such as deposition/filtration, crystallization - suctioning of oil, using Na2SO4 – filtering of oil, Na2SO4 - suctioning of oil, second extraction and combination all the above methods. The results showed that the initial moisture content of the material was 77.37%, and dry matter content was 8.46%. Extraction at optimum conditions is 60 minutes, 1/8 of raw material / solvent ratio (w / v), with fresh ingredients and raw material size is small at 1 ÷ 3.5 mm the highest oil content was obtained. The essential oil obtained contain a high content of Limonene (over 85%), which has been shown to be able to inhibit breast cancer, colorectal cancer, control heartburn and gastric reflux, and support the treatment of gallstones containing cholesterol.

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Optimization of essential oil yield from Vietnamese green pepper (Piper nigrum) using hydro-distillation method

Cited by 14 publications

References 35 publications

Formulation of an Origanum vulgare based dental gel with antimicrobial activity

Formulation of an Origanum vulgare based dental gel with antimicrobial activity

Effects of Ionizing Radiation on Human Peripheral Blood Mononuclear Cells (PBMCs) in the Presence of Mentha-Pulegium Essential Oil: A Study on the Radioprotective Effect

Research methods of refined oil from peel calamondin (Citrus microcarpa) from Tien Giang Province, Vietnam

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