Optimization of ethanol extraction and further purification of isoflavones from soybean sprout cotyledon

Cho, Seung Yong; Lee, Yu Nam; Park, Hyun Jin

doi:10.1016/j.foodchem.2009.04.003

Cited by 53 publications

(52 citation statements)

References 18 publications

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“…Vegetable-type sprouts usually contain quantities of beneficial vitamins and minerals that are found in higher abundance when compared to mature vegetables or dormant seeds. In soybean sprouts, it has been reported that the content of phenolic compounds and isoflavones was higher than that of soybeans (Cho et al 2009;Kim et al 2006). In lentil sprouts, it has been reported that the vitamin C and total phenols are three times higher than in the seeds (Fernandez-Orozco et al 2006;Duenas et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Changes in phenols contents from buckwheat sprouts during growth stage

Koyama

Nakamura

2011

J Food Sci Technol

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Germinated buckwheat is buckwheat seeds soaked in water just until it begins to bud. Buckwheat sprouts are seedling plants of buckwheat grown up to 10-15 cm. The purpose of this study was to determine the optimal growth period for accumulating the most abundant functional phenol(s) in germinated buckwheat that had been soaked in darkness and buckwheat sprouts cultivated by hydroponic culture. The rutin contained in germinated buckwheat was analyzed by CE (capillary electrophoresis). Phenols, including isoorientin, orientin, isovitexin, vitexin, and rutin were separated from buckwheat sprouts by HPLC and identified by LC-MS. The highest rutin content in germinated buckwheat was found to be 15.8 mg/100 g DW at 20 h after germination. Buckwheat sprouts contained five kinds of major phenols. The highest amounts of isoorientin, orientin, isovitexin, and vitexin were measured at day 3, with the exception of rutin, and then a gradual decrease was observed as the sprouts grew. The quantities of isoorientin, orientin, isovitexin, and vitexin at day 3 were 5.8, 11.7, 26.2, and 28.9 mg/100 g FW, respectively. The rutin content rapidly increased to 109.0 mg/100 g FW until day 6. The highest total phenols in buckwheat sprouts were 162.9 mg/100 g FW at day 6. Germinated buckwheat soaked for 20 h and buckwheat sprouts cultivated for 6 days were rich in dietary phenol(s), which makes these plants a valuable functional food for human consumption.

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Section: Introductionmentioning

confidence: 99%

Changes in phenols contents from buckwheat sprouts during growth stage

Koyama

Nakamura

2011

J Food Sci Technol

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“…Researchers have utilized different extraction equipment or techniques such as multi-wrist shaker, rotary shaker, stirring, sonicator, Soxhlet, supercritical fluid extractor, and pressurized liquid extractor for extraction of isoflavones from soybeans (11,12). In addition, a wide variation in the composition of extraction solvent (methanol, ethanol, and acetonitrile with different proportions of acidified and non-acidified water) has been reported (13). Different processes including chromatography, solidphase extraction, liquid-liquid extraction, crystallization, etc., have been employed for the purification and concentration of isoflavones.…”

Section: Introductionmentioning

confidence: 99%

Extraction and Concentration of Isoflavones from Soybean(Glycine max)

Lakshmi

Rao

Ravi

et al. 2013

Separation Science and Technology

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The extraction and concentration of isoflavones from defatted soy flour (DSF) is attempted by leaching, membrane process and liquid-liquid extraction. The optimized extraction conditions by response surface methodology (RSM) resulted in an isoflavone content of 3.2 mg/g of DSF. The extracted isoflavones are processed by ultrafiltration for preliminary purification resulting in 3.0 mg/g of DSF, and then concentrated by liquid-liquid extraction with diethyl ether (4.8 mg/g DSF). The final isoflavone content is 69 mg/g of dried extract with an overall recovery of 57.5%. The optimization of extraction conditions resulted in an about 2.5 fold enhancement of isoflavones and concentration step enriched the isoflavones by about 8.5 fold compared to initial extract.

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“…In germinated brown rice, it has been reported that there is 10 times more GABA (γ-aminobutyric acid), which has an antihypertensive effect, as compared to non-germinated brown rice [17]. Vegetable-type sprouts usually contain quantities of beneficial carbohydrates, proteins, amino acids, vitamins, minerals, and phenolic compounds that are found in higher abundance when compared to mature vegetables or dormant seeds [18]. In soybean sprouts, it has been reported that the content of phenolic compounds and isoflavones is higher than that of soybeans [18,19].…”

mentioning

confidence: 99%

“…Vegetable-type sprouts usually contain quantities of beneficial carbohydrates, proteins, amino acids, vitamins, minerals, and phenolic compounds that are found in higher abundance when compared to mature vegetables or dormant seeds [18]. In soybean sprouts, it has been reported that the content of phenolic compounds and isoflavones is higher than that of soybeans [18,19]. In lentil sprouts, it has been reported that vitamin C and total phenols are three times higher than that of seeds [20].…”

mentioning

confidence: 99%

Quantitative differentiation of phenolic compounds in different varieties of buckwheat cultivars from China, Japan and Korea

Seo¹,

Lee²,

Arasu³

et al. 2013

JACEN

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The aim of this study was to determine the variation of phenolic compounds in common and tartary buckwheats collected from China, Japan and Korea. Two buckwheat varieties of each country were cultivated using nutrient solution and vermiculite in the greenhouse from February to May, 2012 and harvested at twoweek intervals. Phenolics such as chlorogenic acid, C-glycosylflavones (orientin, isoorientin, vitexin, and isovitexin), rutin, and quercetin were quantified using high performance liquid chromatography (HPLC). At 17, 29, 44, 58, and 72 days after sowing (DAS), before harvesting each organs, rutin content of cultivars from China and Korea at 17 DAS was the highest (mean 43.63 and 39.95 mg·g -1 DW, respectively) than that of 29 and 44 DAS. However, two varieties from Japan at 44 DAS were documented maximum; whereas, the amount of rutin in "Hokkai T10" harvested at 44 DAS (58.36 mg·g -1 DW) was the highest of all cultivars. Rutin level in the leaves and stems at 72 DAS were comparatively higher than at 58 DAS. The highest of rutin content was found at 72 DAS (73.33 mg·g -1 DW) in "Hokkai T10" leaves followed by "Daegwan No.3-3" (61.13 mg·g -1 DW), "rice tartary" (53.89 mg·g -1 DW). Rutin content of flowers was presented as the highest amount in "Hokkai T10" at 72 DAS (88.3 mg·g -1 DW) was approximately 14-fold higher than that of 58 DAS (6.44 mg·g -1 DW). However, rutin content of flower in "Xiqiao No.2" was 3-fold higher at 72 DAS than at 58 DAS. Rutin and total phenolic compounds content in flowers, leaves, and stems were higher in the order. The content of four C-glycosylflavones in common buckwheat was higher than those in tartary buckwheat, but rutin content which accounted >90% of the total phenolic compounds was higher in tartary buckwheat. The highest amounts of chlorogenic acid and quercetin were measured in the flower of "Xiqiao No.2" (6.85 and 11.69 mg·g -1 DW, respectively). Based on these results, the presence of different phenolic compounds in all the varieties of buckwheat confirmed that it can be regarded as a potent source of functional foods.

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Optimization of ethanol extraction and further purification of isoflavones from soybean sprout cotyledon

Cited by 53 publications

References 18 publications

Changes in phenols contents from buckwheat sprouts during growth stage

Changes in phenols contents from buckwheat sprouts during growth stage

Extraction and Concentration of Isoflavones from Soybean(Glycine max)

Quantitative differentiation of phenolic compounds in different varieties of buckwheat cultivars from China, Japan and Korea

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