Optimization of Extraction Conditions and Characterization of Volatile Organic Compounds of Eugenia klotzschiana O. Berg Fruit Pulp

Abstract

Eugenia klotzschiana O. Berg is a native species to the Cerrado biome with significant nutritional value. However, its volatile organic compounds (VOCs) chemical profile is not reported in the scientific literature. VOCs are low molecular weight chemical compounds capable of conferring aroma to fruit, constituting quality markers, and participating in the maintenance and preservation of fruit species. This work studied and determined the best conditions for extraction and analysis of VOCs from the pulp of Eugenia klotzschiana O. Berg fruit and identified and characterized its aroma. Headspace solid-phase microextraction (HS-SPME) was employed using different fiber sorbents: DVB/CAR/PDMS, PDMS/DVB, and PA. Gas chromatography and mass spectrometry (GC-MS) were employed to separate, detect, and identify VOCs. Variables of time and temperature of extraction and sample weight distinctly influenced the extraction of volatiles for each fiber. PDMS/DVB was the most efficient, followed by PA and CAR/PDMS/DVB. Thirty-eight compounds that comprise the aroma were identified among sesquiterpenes (56.4%) and monoterpenes (30.8%), such as α-fenchene, guaiol, globulol, α-muurolene, γ-himachalene, α-pinene, γ-elemene, and patchoulene.

Volatile Organic Compounds and Physiological Parameters as Markers of Potato (Solanum tuberosum L.) Infection with Phytopathogens

“…Except strain specify, volatile production is also influenced by growth medium, incubation time, humidity, and temperature [7]. In addition, for plant VOC, this volatile compound profile is specific for different varieties of the same plant species [8].…”

“…Headspace solid-phase microextraction (HS-SPME) is used for complex matrices analysis. It does not destruct the analyzed sample and does not require the use of organic solvents [8].…”

Profile of Myracrodruon urundeuva Volatile Compounds Ease of Extraction and Biodegradability and In Silico Evaluation of Their Interactions with COX-1 and iNOS

“…SPME consists of the analyte partitioning between the sample and an extracting microcomponent, constituting a polymeric phase that can be solid or liquid that involves a fused silica fiber [ 32 ]. Relatively to the advantages of this technique, it is noteworthy that it does not demand sophisticated analytical tools, in addition to organic solvents dispensing and enabling the reuse of the extraction fibers [ 33 , 34 , 35 , 36 ]. This technique also presents itself widely dependent on the type of fiber.…”

“…This technique also presents itself widely dependent on the type of fiber. It was shown in the work of [ 36 ] that the PDMS/DVB fiber allowed the identification of more than twice the volatile compounds than the DVB/CAR/PDMS fiber for the pulp of Eugenia klotzchiana O.…”

The Presence of Flavonoids in Some Products and Fruits of the Genus Eugenia: An Integrative Review

“…Regarding the consumption of E. klotzschiana fruits (Figure 4) in natura, knowing their maturation stages is essential to establish their shelf-life and post-harvest quality (Carneiro, 2015;Sanches et al, 2021). It has significant nutritional value, including the content of polyphenolic compounds and flavonoids, in addition to a considerable content of ascorbic acid and dietary fiber (Mariano et al, 2022).…”