Optimization of Extraction Conditions for Determination of Tiliroside in Tilia L. Flowers Using an LC-ESI-MS/MS Method

Abstract

Tiliroside exhibits a wide spectrum of effects on the human body; considering expensive synthesis of tiliroside, linden trees seem to be a good source of this compound. For the first time, 46 various extraction methods were developed to receive tiliroside from Tilia L., including ultrasound-assisted extraction, maceration, maceration with stirring, accelerated solvent extraction, and extraction under reflux. The effects of extraction techniques, solvents, additives, and temperature on the content of tiliroside were studied using analytical and statistical methods. A new, rapid, simple, sensitive, and selective liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) method was developed to determine the content of tiliroside in Tilia L. flowers. The LC-ESI-MS/MS analysis demonstrated the highest content of tiliroside in Tilia L. flowers obtained using accelerated solvent extraction (ASE) where 70% ethanol with addition of 1% acetic acid was used as a solvent (7.400 ± 0.019 mg of tiliroside per g dry extract).The results showed that the extracts of Tiliae inflorescentia contained large amounts of tiliroside; therefore, they are good sources of this compound. Moreover, ASE was found to be superior to other extraction techniques due to its high efficiency as well as considerable saving of time and solvent.

Impact of Harvest Conditions and Host Tree Species on Chemical Composition and Antioxidant Activity of Extracts from Viscum album L.

“…The antiradical activity was assayed using an improved ABTS +• decolorization assay with modifications [ 40 , 41 ]. The absorbance was measured at 734 nm after a 6-min incubation.…”

Ligand fishing based on tubular microchannel modified with monoamine oxidase B for screening of the enzyme's inhibitors from Crocus sativus and Edgeworthia gardneri

“…The chemical structure of compound 1 was identified with MS/MS (Figure S1). It showed a quasi‐molecular ion at m/z 593.1 [M–H] – in the MS spectrum, and the molecular formula was suggested to be C 30 H 26 O 13 by comparison with the previous literature [41] (Structure shown in Figure S3A). In the MS/MS spectrum, two daughter ions at m/z 446.9 [M–H−146] − and 284.6 [M–H−146−162] − were ascribable to the fission of sites 1 and 2 in the molecule (Figure S3B).…”

DNA-Based Authentication and Metabolomics Analysis of Medicinal Plants Samples by DNA Barcoding and Ultra-High-Performance Liquid Chromatography/Triple Quadrupole Mass Spectrometry (UHPLC-MS)

“…The rest of linden samples had an average of 0.034%. There are no data on tiliroside content neither in the European Medicines Agency nor in the Real Spanish Pharmacopeia [ 43 ]. The content of tiliroside has been identified to be higher in inflorescences that in leaves in Tilia cordata (49.2 µg/g versus 16.1 µg/g) [ 44 ].…”