Optimization of Extraction Method and Characterization of Phycocyanin Pigment from Spirulina platensis

Julianti, Elin; Susanti, Susanti; Wibowo, Marlia Singgih; Mulyani, Laida Neti

doi:10.5614/j.math.fund.sci.2019.51.2.6

Cited by 20 publications

(7 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This process was repeated 2-3 times. After the freeze-thawing cell disruption period, samples were centrifuged, and aliquots were separated to analyze phycocyanin by UV-Vis spectroscopy (Thermo Fisher Scientific, Waltham, MA, USA) [19]. A UV-Vis spectrophotometer was used to measure the absorbance of the extracts at wavelengths 280, 615, 620, and 652 nm, respectively.…”

Section: Extraction Of Chlorophyll-a and Chlorophyll-b Total Caroteno...mentioning

confidence: 99%

Strategy Development for Microalgae Spirulina platensis Biomass Cultivation in a Bubble Photobioreactor to Promote High Carbohydrate Content

et al. 2022

View full text Add to dashboard Cite

As a counter to climate change, energy crises, and global warming, microalgal biomass has gained a lot of interest as a sustainable and environmentally favorable biofuel feedstock. Microalgal carbohydrate is considered one of the promising feedstocks for biofuel produced via the bioconversion route under a biorefinery system. However, the present culture technique, which uses a commercial medium, has poor biomass and carbohydrate productivity, creating a bottleneck for long-term microalgal-carbohydrate-based biofuel generation. This current investigation aims toward the simultaneous increase in biomass and carbohydrate accumulation of Spirulina platensis by formulating an optimal growth condition under different concentrations of nitrogen and phosphorous in flasks and a bubble photobioreactor. For this purpose, the lack of nitrogen (NaNO3) and phosphorous (K2HPO4) in the culture medium resulted in an enhanced Spirulina platensis biomass and total carbohydrate 0.93 ± 0.00 g/L and 74.44% (w/w), respectively. This research is a significant step in defining culture conditions that might be used to tune the carbohydrate content of Spirulina.

show abstract

Section: Extraction Of Chlorophyll-a and Chlorophyll-b Total Caroteno...mentioning

confidence: 99%

Strategy Development for Microalgae Spirulina platensis Biomass Cultivation in a Bubble Photobioreactor to Promote High Carbohydrate Content

et al. 2022

View full text Add to dashboard Cite

show abstract

“…An acetate buffer at a pH of 5 is occasionally employed as a buffer for C-PE extraction [ 86 ]. However, research by Julianti et al (2019) and Zavrel et al (2018) demonstrated that the C-PC yield and purity extracted with an acetate buffer are lower than that extracted with a phosphate buffer [ 87 , 88 ]. A comparatively low-cost, double-distilled water was shown to extract the highest quantity of C-PC, with no significant difference in comparison to PC amounts extracted using a phosphate buffer [ 89 ].…”

Section: Downstream Processing Of C-pementioning

confidence: 99%

A Review on a Hidden Gem: Phycoerythrin from Blue-Green Algae

et al. 2022

View full text Add to dashboard Cite

Phycoerythrin (PE) is a pink/red-colored pigment found in rhodophytes, cryptophytes, and blue-green algae (cyanobacteria). The interest in PE is emerging from its role in delivering health benefits. Unfortunately, the current cyanobacterial-PE (C-PE) knowledge is still in the infant stage. It is essential to acquire a more comprehensive understanding of C-PE. This study aimed to review the C-PE structure, up and downstream processes of C-PE, application of C-PE, and strategies to enhance its stability and market value. In addition, this study also presented a strengths, weaknesses, opportunities, and threats (SWOT) analysis on C-PE. Cyanobacteria appeared to be the more promising PE producers compared to rhodophytes, cryptophytes, and macroalgae. Green/blue light is preferred to accumulate higher PE content in cyanobacteria. Currently, the prominent C-PE extraction method is repeated freezing–thawing. A combination of precipitation and chromatography approaches is proposed to obtain greater purity of C-PE. C-PE has been widely exploited in various fields, such as nutraceuticals, pharmaceuticals, therapeutics, cosmetics, biotechnology, food, and feed, owing to its bioactivities and fluorescent properties. This review provides insight into the state-of-art nature of C-PE and advances a step further in commercializing this prospective pigment.

show abstract

“…The US Food and Drug Administration (FDA) has regulations to employ the phycocyanin pigment obtained from Spirulina platensis as color additive in gum, toffee and other food items (Julianti et al, 2019;Spolaore et al, 2006).…”

Section: Pigmentsmentioning

confidence: 99%

Microalgae for biotechnological applications: Cultivation, harvesting and biomass processing

et al. 2020

View full text Add to dashboard Cite

Optimization of Extraction Method and Characterization of Phycocyanin Pigment from Spirulina platensis

Cited by 20 publications

References 15 publications

Strategy Development for Microalgae Spirulina platensis Biomass Cultivation in a Bubble Photobioreactor to Promote High Carbohydrate Content

Strategy Development for Microalgae Spirulina platensis Biomass Cultivation in a Bubble Photobioreactor to Promote High Carbohydrate Content

A Review on a Hidden Gem: Phycoerythrin from Blue-Green Algae

Microalgae for biotechnological applications: Cultivation, harvesting and biomass processing

Contact Info

Product

Resources

About