Optimization of Extraction of Lipase from Wheat Seeds (Triticum aestivum) by Response Surface Methodology

Pierozan, Morgana Karin; Costa, Roger Junges da; Antunes, O. A. C.; Oestreicher, Enrique G.; Oliveira, J. Vladimir; Cansian, Rogério Luís; Treichel, Helen; Oliveira, Débora de

doi:10.1021/jf901816x

Cited by 24 publications

(12 citation statements)

References 24 publications

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“…The results indicated that the RSM model used to fit the response variable was signifi cant (p = 0.0135) and adequate to represent the relationship between the response and the independent variables (12,13). The model demonstrated a very high F-value (F = 6.0442).…”

Section: Optimiszation Of Response Surface Methodology (Rsm)mentioning

confidence: 92%

Evaluation of the Antioxidant Activity of Coreopsis Tinctoria Nuff. and Optimisation of Isolation by Response Surface Methodology

Lan

Lin

2014

Acta Pharmaceutica

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Evaluation of the antioxidant activity of Coreopsis tinctoria Nuff. and optimisation of isolation by response surface methodologyThe aim of the present study was to determine the antioxidant activity of Coreopsis tinctoria flowering tops (CTFT). Studies were conducted to obtain suitable extraction conditions for chlorogenic acid, quercetin, luteolin, apigenin and kaempferol, which were identified and quantified by HPLC. Response surface methodology was employed to optimise the ultrasound-assisted extraction conditions including extraction time, ethanol concentration and liquid-solid ratio. The antioxidant activity of the extracts was analysed using various antioxidant models, such as DPPH, ABTS and hydroxyl radical scavenging assay. CTFT extracted for 15.0 min with ethanol at a concentration of 60.4 % and with liquidsolid ratio 27.5:1 possessed a considerable amounts of total flavonoids and polyphenols (18.9 %). This extract showed higher scavenging activity of ABTS and hydroxyl radical activity than rutin, however not in the DPPH test. We may assume that CTFT possess antioxidant and free radical scavenging potentials.

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Section: Optimiszation Of Response Surface Methodology (Rsm)mentioning

confidence: 92%

Evaluation of the Antioxidant Activity of Coreopsis Tinctoria Nuff. and Optimisation of Isolation by Response Surface Methodology

Lan

Lin

2014

Acta Pharmaceutica

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“…The higher hydrolytic activity was 5.9 U/g with a particle size of 425 μm, solid/solvent ratio of 30:60 (w/v), and reaction time of 15 h, with maximum hydrolytic activity of 14.47 U/g after 24 h of precipitation. For esterification activity, the best result was 57.88 U/g with a particle size of 425 μm and solid/solvent ratio of 30:120 (w/v) for 5 h, with a maximum value after 10 h of precipitation reaching 208.20 U/g [12].…”

Section: Extraction Of Lipase From Wheat Seedsmentioning

confidence: 99%

“…The choice of this raw material was based on a previous work by our research group [12] where we have evaluated the hydrolytic lipase activity using wheat germ and wheat seed as lipase sources. The results obtained permitted us to observe that the hydrolytic activities for lipases obtained from wheat germ were, in general, lower than that obtained when using wheat seed as the enzyme source.…”

Section: Extraction Of Lipase From Wheat Seedsmentioning

confidence: 99%

Studies on Immobilization and Partial Characterization of Lipases from Wheat Seeds (Triticum aestivum)

Pierozan

Oestreicher

Oliveira

et al. 2011

Appl Biochem Biotechnol

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The objective of this study was to provide some features on immobilization and partial characterization of lipases from wheat seeds. The optimum pH and temperature were found to be 5.5 and 32-37 °C, respectively. The stability of the concentrated enzymatic extract to high temperatures (25, 35, 45, and 55 °C) showed that the incubation of the extract at 55 °C led to its complete inactivation. The concentrated enzymatic extract kept 90% of its hydrolytic and esterification activities until 70 and 40 days of storage at 4 °C, respectively. The extract presented higher hydrolytic specificity to substrates of medium and long chains and higher esterification affinity to fatty acids of short and medium chains and alcohols with two and three carbon atoms. After the immobilization process using activated coal and sodium alginate as supports, an enhancement of about threefold in lipase activity was observed. The development of the present work permitted us to point out some characteristics of lipases from wheat seeds necessary for the proposition of new future industrial applications for this important biocatalyst.

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Section: Introductionmentioning

confidence: 99%

“…In this study, saponin extract from C. oleifera cake has been prepared using response surface methodology (RSM), which has been reported to be an effective tool for optimising the process (Pierozan et al, 2009). The identification of the saponins obtained was carried out by HPLC-ESI-MS, and the possible structures of these saponins were further analysed.…”

Section: Introductionmentioning

confidence: 99%

Extraction of saponin from Camellia oleifera cake and evaluation of its antioxidant activity

Nie²,

Huang

et al. 2012

Int J of Food Sci Tech

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Summary Response surface methodology was employed to optimise extraction conditions of saponins from Camellia oleifera cake. Optimal conditions were extraction at 82.2 °C for 3.3 h with an 8.6:1 ratio (mL g−1) of solvent to solid. The saponin extract was further extracted with n‐butanol, and nine saponins were found as the main components. The identification of these saponins was carried out by HPLC–ESI–MS, and their possible structures were given. Antioxidant activities of the saponins were evaluated in vitro by 1,1‐diphenyl‐2‐picryl‐hydrazyl (DPPH), 1, 2, 3‐phentriol self‐oxidation and metal chelating activity assays. IC50 value of each assay was 3866 ± 3, 4744 ± 2 and 2389 ± 2 μg mL−1, respectively. The effects of environmental factors on antioxidant activity of saponins from C. oleifera cake were studied for the first time. The antioxidant activity is pH dependent with 9.0 as the optimal pH and is also temperature independent.

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Optimization of Extraction of Lipase from Wheat Seeds (Triticum aestivum) by Response Surface Methodology

Cited by 24 publications

References 24 publications

Evaluation of the Antioxidant Activity of Coreopsis Tinctoria Nuff. and Optimisation of Isolation by Response Surface Methodology

Evaluation of the Antioxidant Activity of Coreopsis Tinctoria Nuff. and Optimisation of Isolation by Response Surface Methodology

Studies on Immobilization and Partial Characterization of Lipases from Wheat Seeds (Triticum aestivum)

Extraction of saponin from Camellia oleifera cake and evaluation of its antioxidant activity

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