Optimization of extraction technology for determination of caffeic and chlorogenic acid in dandelion

Wu, Zhe; Li, Zhaojia; Zhi-zhong, Xue; Wang, Xiuping

doi:10.7904/2068-4738-xi(21)-26

Cited by 5 publications

(6 citation statements)

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“…The compounds in marigold leaf extract were determined by ultra performance liquid chromatography (Waters UPC2/I-Class UPLC, USA) equipped with ZORBAX Eclipse Plus C18 column (3.0 × 150 mm, 1.8 µm) under a mobile phase of 0.1% formic acid water (A) and acetonitrile (B) by the gradient elution as the method described [12,16]. Cell density was directly counted by a hemocytometer, and then cell growth inhibitory rate was calculated as the rate of decreased cell density in marigold leaf extract treatments compared to that of control treatment.…”

Section: Determination Methodsmentioning

confidence: 99%

“…Leaves were cleaned, dried, crushed, and then passed through a 0.25 mm sieve. 75 g fine powder was mixed with 500 ml of 0.1% cellulase solution (cellulase activity 5,000 U/g, cellulase content 0.5 g, and solution pH = 4) and extracted 30 minutes at 60ºC following the extraction method described previously [12]. The water extract was filtered by 0.45 µm membrane and condensed to 300 ml by a Termovap-Sample Concentrator (QF-3800, Shanghai Forthright Bio.…”

Section: Marigold Leaf Extractionmentioning

confidence: 99%

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Allelopathic effect of marigold (Tagetes erecta L.) leaf extract on growth of Chlorella vulgaris

Wongsnansilp¹,

Phinrub²,

Juntawong³

2022

J Appl Biol Biotech

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Marigold (Tagetes erecta L.) is a perennial edible medicinal plant. In order to test the allelopathic effect of marigold leaf extract on Chlorella vulgaris, the microalgae growth conditions under different concentrations of marigold leaf extract (0 to 50 g/l) were studied. The results showed that microalgal growth was inhibited by marigold leaf extract, and inhibition commonly increased with increasing concentration of the extract and culture time. The maximum inhibition rate (90.1%) appeared in leaf extract of 30 g/l after 14 days of incubation; marigold leaf extract induced chlorophyll degradation in algae cells, increased the permeability of cell membrane, and caused exudation of soluble protein and nucleic acid, leading to the damage of algae cell structure and metabolic dysfunction. The present results confirmed that marigold leaf extract has an allelopathic inhibition effect on C. vulgaris, and the results could be further applied to develop safe and efficient algaecides.

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Section: Determination Methodsmentioning

confidence: 99%

Section: Marigold Leaf Extractionmentioning

confidence: 99%

Allelopathic effect of marigold (Tagetes erecta L.) leaf extract on growth of Chlorella vulgaris

Wongsnansilp¹,

Phinrub²,

Juntawong³

2022

J Appl Biol Biotech

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“…Dandelion is a common Chinese herbal. Ferulic acid (FA), chlorogenic acid (CLA), and caffeic acid (CA) with the properties of antiinflammation and disinfection are the representative components of dandelion . It is crucial to test the representative components for the quality assessment of dandelion.…”

Section: Introductionmentioning

confidence: 99%

“…Ferulic acid (FA), chlorogenic acid (CLA), and caffeic acid (CA) with the properties of antiinflammation and disinfection are the representative components of dandelion. 23 It is crucial to test the representative components for the quality assessment of dandelion. The traditional sample pretreatment of dandelion mainly including liquid−liquid extraction, liquid−solid extraction, and microwave-assisted extraction mostly requires lots of expensive and toxic organic solvents, and the operation process is time-consuming.…”

Section: Introductionmentioning

confidence: 99%

Facile Cloud Point Extraction for the Separation and Determination of Phenolic Acids from Dandelion

et al. 2021

ACS Omega

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It is significantly crucial to develop a robust pretreatment for the quantitative analysis of herbs. However, the traditional strategies are time-consuming, tedious, and not eco-friendly. In this work, cloud point extraction (CPE) is engineered for the simultaneous separation and enrichment of ferulic acid (FA), chlorogenic acid (CLA), and caffeic acid (CA) from dandelion prior to its determination by high-performance liquid chromatography (HPLC). A famous nonionic surfactant of Triton X-114 was selected as an extractant of CPE, and parameters affecting the extraction, such as surfactant concentration, salt content, pH value, temperature, and incubation time, were investigated carefully. Furthermore, the well-designed CPE with ultrasonic assistance combined with HPLC was developed for the detection of the target analytes in dandelion. The established method having a good linearity in the range of 0.15–26.2 mg L–1 with R 2 more than 0.9979 and the spiked recoveries ranging from 81 to 96% was applied to test real samples of dandelion. The contents of CA in samples were consistent with those assayed by the method (Chinese Pharmacopoeia 2015). The proposed method afforded good analytical performances, shorter pretreatment time (65 min), and less organic solvent consumption (less than 1.0 mL). It was proved that the developed method presented a facile, inexpensive, efficient, and environment-friendly pretreatment and can be used for the quantitative analysis of CLA, CA, and FA in dandelion. As expected, the proposed method would be a promising potential for the quality analysis of herbal medicines.

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“…Caffeic acid (3,4-dihydroxycinnamic acid, Figure 1) is a hydroxycinnamic acid derivative with potential antioxidant, anti-inflammatory, and antihyperglycemic action and antineoplastic activities [14]. It is a naturally occurring phenolic compound which is produced in many fruits, vegetables, and herbs, including pears, basil, thyme, verbena, tarragon, oregano, wood betony, burning bush, turmeric, dandelion, yarrow, horsetail, rosemary, hawthorn, and coffee [15]. The amount of caffeic acid is strongly dependent on the plant species [16].…”

Section: Introductionmentioning

confidence: 99%

Effective Lipid Nanocarriers Based on Linseed Oil for Delivery of Natural Polyphenolic Active

Coc

Lăcătuşu

Badea

et al. 2021

Journal of Nanomaterials

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The main purpose of the present research was to test the ability of nanostructured lipid carriers (NLCs) to efficiently host a hydrophilic polyphenol active with health-promoting activities (caffeic acid (CA)). The caffeic acid-loaded lipid nanocarriers (CA-NLCs) were obtained by high-pressure homogenization technique using a surfactant mixture of Tween 20 and L-α-phosphatidylcholine in association with a lipid mixture of linseed oil, hexadecyl palmitate, and glycerol monostearate. In the first stage, the proportion between surfactant mixture and lipid phase has been varied to obtain appropriate stable nanocarriers. The optimized NLCs have been further loaded with different amounts of caffeic acid and were analyzed in terms of physical stability, size characteristics, and encapsulation efficiency. The antioxidant activity of CA-loaded NLCs and their release behavior have been tested by specific in vitro methods, e.g., ABTS (2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)) assay and release experiments, by Franz cell diffusion. The ABTS assay highlighted a high antioxidant potential of the caffeic acid in association with linseed oil. The capacity to capture ABTS cationic radicals was superior for the NLC entrapping an initial amount of 1.5% CA, the level of antioxidant capacity being 91.3%. The in vitro release experiments showed a different release behavior, depending on the initial amount of caffeic acid used. NLC loaded with a higher concentration of CA manifests a gradual slow release, e.g., 45% CA after 24 h of in vitro experiments, while the NLC loaded with smaller concentration of CA assured a higher release in time, around 65%.

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Optimization of extraction technology for determination of caffeic and chlorogenic acid in dandelion

Cited by 5 publications

References 0 publications

Allelopathic effect of marigold (Tagetes erecta L.) leaf extract on growth of Chlorella vulgaris

Allelopathic effect of marigold (Tagetes erecta L.) leaf extract on growth of Chlorella vulgaris

Facile Cloud Point Extraction for the Separation and Determination of Phenolic Acids from Dandelion

Effective Lipid Nanocarriers Based on Linseed Oil for Delivery of Natural Polyphenolic Active

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