Optimization of extrusion-assisted extraction parameters and characterization of alginate from brown algae (Sargassum cristaefolium)

Abstract

The aim of this research is to investigate the effects of brown algae to solution ratio, feed rate, and pH on the multiple responses of Sargassum cristaefolium alginate extracted using twin screw extruder. Box-Behnken design was used to find out the optimum extrusionassisted extraction parameters based on the responses of residence time distribution (RTD), yield, intrinsic viscosity, and molecular weight. The result showed that alginate extrusion-assisted extraction parameters affected on the movement of algae in the screw channel and physicochemical properties of S. cristaefolium alginate. The alginate extrusion-assisted extraction parameters have quadratic effect on the responses of RTD, yield, intrinsic viscosity and molecular weight. The predicted values at the optimum extrusion parameters as independent variables are the use of brown algae to solution ratio (3.11), feed rate (2.95 rpm), and pH 10.3. The M/G ratio of S. cristaefolium alginate based on fractions analysis is 0.29 (M/G ratio \ 1), indicating that S. cristaefolium alginate contains guluronate fraction of 77.10% and manuronate fraction of 22.90%. Intrinsic viscosity of S. cristaefolium alginate in aqueous solution was determined and shown shear-thinning pseudoplastic.

Relevance of the Extraction Stage on the Anti-Inflammatory Action of Fucoidans

“…Ultrasound assistance was used to extract phenolics and fucoidans from Ascophylum nodosum during acid extraction [ 156 ] and fucoidans from Undaria pinnatifida , which resulted in increased yield and decreased extraction time [ 147 ]. This stage could also be proposed as a pretreatment, as reported from Sargassum polycystum (amplitude 80%, 15 min) before acid extraction [ 157 ]. Following ultrasonication, the average molecular weight of the fucoidans decreased [ 158 ] and the anti-inflammatory action of U. pinnatifida [ 147 ] and the immunomodulatory potential of Hizikia fusiforme [ 5 ] fucoidans were enhanced.…”

Algal Alginate in Biotechnology: Biosynthesis and Applications

“…Therefore, novel approaches are suggested from several studies for the development of more suitable and effective extraction process (Figure 3). Sugiono et al [40] performed an extrusion-assisted extraction procedure and optimized the key parameters (brown algae: solution ratio, feed rate and pH) for the alginate extraction from Sargassum cristaefolium. They reported that the extraction yield at optimum conditions reached the value of 34.96 ± 0.09%, and twin screw extruder was a promising method to extract alginate at the industrial scale.…”

Efek Penggunaan Pelarut Berulang pada Proses Pre-Ekstraksi Terhadap Mutu Alginat dari Sargassum cristaefolium

“…The number of solvent recycling dan rapuh, sedangkan alginat dengan rasio M/G tinggi lebih besar dari 1 bersifat lebih elastis dan membentuk larutan yang encer (Sugiono et al, 2019;Bertagnolli et al, 2014). Metode ekstraksi yang dapat menghasilkan alginat dengan nilai viskositas yang tinggi maka proses tersebut semakin baik dan efektif.…”

“…Tingkat degradasi oligoguluronat lebih cepat dari oligomanuronat dengan semakin meningkat pH (Smidsrod et al 1969). Sifat reologi larutan alginat sangat dipengaruhi oleh rasio M/G, alginat dengan rasio M/G lebih dari 1 bersifat lebih fleksibel dengan viskositas relatif rendah, sedangkan alginat dengan rasio M/G kurang dari 1 lebih bersifat kaku dengan viskositas lebih tinggi (Torres et al 2007;Sugiono et al 2019). Jayasankar (1993) dan Gambar 2.…”