Optimization of fermentation conditions for ethanol production from whey

Castillo, Francisco; Izaguirre, Marlene E.; Michelena, V.; Moreno, Belisario

doi:10.1007/bf00127786

Cited by 11 publications

(2 citation statements)

References 16 publications

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“…Response surface methodology (RSM) is one such scientific approach that is useful for developing, improving and optimizing processes and is used to analyze the effects of several independent variables on the system response, main objective being the determination of optimum operational conditions within the operating specifications (Ravikumar et al, 2005;Jo et al, 2008). This method has been successfully applied to optimize alcoholic fermentation process (Castillo et al, 1982;Ratnam et al, 2003). In the present study, the RSM based central composite design (CCD) was applied to identify the optimum condition of the FWL fermentation (Le Man et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Optimization of operational parameters for ethanol production from Korean food waste leachate

Man

Behera

Park

2009

Int. J. Environ. Sci. Technol.

161

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ABSTRACT:Recently, research on the production of ethanol from waste has been accelerating for both ecological and economical reasons, primarily for its use as an alternative to petroleum based fuels. In this study, response surface methodology based 2 3 -full factorial central composite design was employed to optimize the parameters of ethanol production from Korean food waste leachate. The reducing sugar concentration of the food waste leachate determined by the dinitrosalicylic acid method was 75 g/L. A second order polynomial model was developed to evaluate the quantitative effects of temperature, pH and reducing sugar concentration in order to find an optimum condition for the ethanol production from food waste leachate. From the experimental result, maximum ethanol concentration of 24.17 g/L was obtained at the optimum condition of temperature (38 ºC), pH (5.45) and reducing sugar concentration (75 g/L). The experimental value (24.17 g/L) agreed very well with the predicted one (23.66 g/L), indicating the suitability of the model employed and the success of response surface methodology in optimizing the conditions of ethanol production from food waste leachate. Canonical analysis indicated that the stationary point was a saddle point for the ethanol yield. Despite being a waste, an ethanol yield of 0.32 g ethanol/g reducing sugar demonstrated the potential of food waste leachate as a promising biomass resource for the production of ethanol.

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Section: Introductionmentioning

confidence: 99%

Optimization of operational parameters for ethanol production from Korean food waste leachate

Man

Behera

Park

2009

Int. J. Environ. Sci. Technol.

161

View full text Add to dashboard Cite

show abstract

“…Response Surface Methodology (RSM) is one such scientific approach that is useful for developing, improving and optimizing processes and is used to analyze the effects of several independent variables on the system response. This method has been successfully applied to optimize alcoholic fermentation process (Castillo et al, 1982;Ratnam et al, 2003). The present study reports for the first time the new strain combination of Z. mobilis and C. shehatae for ethanol production from food wastes hydrolysates and to determine the optimum level of fermentation variables, nitrogen source [(NH 4 ) 2 SO 4 ], phosphorus source (KH 2 PO 4 ), yeast extract and inoculum size for ethanol production under non-sterilized condition using RSM.…”

Section: Introductionmentioning

confidence: 99%

Optimization of ethanol production from food waste hydrolysate by co-culture of Zymomonas mobilis and Candida shehatae under non-sterile condition

Thongdumyu¹,

Intrasungkha²,

O‐Thong³

2014

Afr. J. Biotechnol.

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A complete conversion of the hexose and pentose sugars in the food wastes hydrolysates (FWH) to ethanol is a prerequisite for maximizing the profitability of an industrial process for bioethanol production. Response surface methodology (RSM) was employed to optimize the effects of nitrogen source [(NH 4) 2 SO 4 ], phosphorus source (KH 2 PO 4), yeast extract and inoculum size on ethanol production from FWH by co-culture of Zymomonas mobilis and Candida shehatae under non-sterile condition. The optimal conditions for ethanol production were 1.15 g/L of (NH 4) 2 SO 4 , 0.95 g/L of KH 2 PO 4 , 1.38 g/L of yeast extract and 14.75%v/v of inoculum. The results indicate that the most significant parameters affecting ethanol production from FWH by co-culture under non-sterile condition was yeast extract. Ethanol production of 77.6 g/L obtained under optimized condition was 56% increased as compared with the use of raw FWH (34 g/L) and was in good agreement with the value predicted by quadratic model (79.98 g/L), thereby confirming its validity. Ethanol yield of FWH in batch fermentation by co-culture was 0.15 g-ethanol/g-food waste (77.6 g/L), which was 94.6% of the theoretical yield while Z. mobilis alone yielded 0.11 g-ethanol/g-food waste (54.2 g/L) and C. shehatae alone yielded 0.09 gethanol/g-food waste (48 g/L). Ethanol production from FWH in 1-L fermentor by co-culture also gave ethanol yield of 0.16 g-ethanol/g-food waste (78.8 g/L) which was 96% of the theoretical yield. Despite of being a waste, an ethanol yield of 0.16 g-ethanol/g-food waste demonstrated the potential of food waste as a promising biomass resource for ethanol production.

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Production of biomas and β‐D‐galactosidase by Candida pseudotropicalis grown in continuous culture on whey

Gómez

Castillo

1983

Biotech & Bioengineering

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The production of biomass and beta-D-galactosidase by the lactose-utilizing yeast Candida pseudotropicalis NCYC 744 in whey medium was studied. Apparent optimization of growth conditions and medium was done in continuous culture. Optimal pH and temperature were 2.6 and 36-38 degrees C, respectively, Limitations in Cu, Zn, and possibly Mn were detected in deproteinized whey medium. Additions of tryptophan stimulated growth of the yeast. Under optimal conditions in medium supplemented with excess tryptophan, Cu, Zn, and Mn the maximum values obtained: yeast concentration, 4.6 g/L; yeast productivity, 1.4 g/L h (at D = 0.35 h(-1)); enzyme volumetric productivity, 2100 U/L h (at D = 0.25 h(-1)); maintenance coefficient, 5-10 mg lactose/g cell h; saturation constant (K(s)) for lactose, 4.76mM; maximum specific growth rate, (mu(max)), 0.47 h(-1). No significant increase in specific enzyme activity (U/mg cell) was observed after medium optimization evidencing the importance of regulatory controls in enzyme synthesis.

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Optimization of fermentation conditions for ethanol production from whey

Cited by 11 publications

References 16 publications

Optimization of operational parameters for ethanol production from Korean food waste leachate

Optimization of operational parameters for ethanol production from Korean food waste leachate

Optimization of ethanol production from food waste hydrolysate by co-culture of Zymomonas mobilis and Candida shehatae under non-sterile condition

Production of biomas and β‐D‐galactosidase by Candida pseudotropicalis grown in continuous culture on whey

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