Optimization of grapevine clonal micropropagation

Abstract

Clonal micro-propagation ensures the production of genetically homogeneous, healthy virus-free planting material. This kind of propagation is influenced by genetic, physiological, hormonal, physical factors, and plant genotype. To increase the success of the process, it is necessary to optimize the methods of clonal micropropagation. The technology of clonal micropropagation of grapevine using a culture of apical meristems with a size of 0.1-0.2 mm was developed, the scheme of plant regeneration was improved, a new biotechnological techniques were developed for all stages of propagation. A method of processing of meristems with an ultrahigh frequency (microwave) electromagnetic field (EMF) in combination with a narrow-band laser was developed. Method for improving recovery from viral and bacterial infections using the growth regulator Emistim, Salicylic acid, the antibiotics Gentamicin and Cefotaxime was found. A method of water therapy was developed. The optimal parameters of intensity and duration of illumination were established. Ways of adaptation of healthy plants to non-sterile environmental conditions were optimised. Methods of testing of viral infection was improved. Techniques of planting healthy seedlings in film, greenhouses and open ground were developed. The creation of unique basic vine nursery in Ust-Donetsk region was the result of conducted studies.