Optimization of Hydrogen Peroxide Detection for a  Methyl Mercaptan Biosensor

Li, Zhanhong; Guedri, Houssemeddine; Viguier, Bruno; Sun, Shi‐Gang; Marty, Jean‐Louis

doi:10.3390/s130405028

Cited by 20 publications

(12 citation statements)

References 37 publications

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“…Hepes buffer solutions were prepared in deionized distilled water. PPD/PB/CSPEs were prepared by electrodeposition as described in 6 (Supporting Information). GAOx@laponite/PPD/PB/CSPEs ( BioElectrode 1 ) was prepared following the same protocol described elsewhere 2.…”

Section: Methodsmentioning

confidence: 99%

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Galactose Oxidase/Prussian Blue Based Biosensors

et al. 2015

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This paper describes the development of an amperometric biosensor based on galactose oxidase (GAOx) immobilization within a laponite clay film deposited on Carbon Screen‐Printed Electrodes modified by electrodeposited Prussian Blue and coated with poly‐(O‐phenylenediamine) (PPD/PB/CSPEs). Amperometric performances of GAOx@laponite/PPD/PB/CSPEs bioelectrodes were determined using several GAOx substrates. Using these modified electrodes the reduction of enzymatically generated hydrogen peroxide was performed at −0.2 V vs. Ag‐AgCl. In an initial attempt, E.Coli transketolase activity on its immobilized form was followed using a bienzymatic GAOx‐TK biosensor.

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Section: Methodsmentioning

confidence: 99%

“…In the present work, Carbon Screen‐Printed Electrodes modified by electrodeposited Prussian Blue (PB/CSPEs) were used as sensitive transducers for H 2 O 2 detection 5. To improve their stability, those PB/CSPEs were protected using a poly‐( O ‐phenylenediamine) (PPD) film 6.…”

Section: Amperometric Performances Of Bioelectrode 1 For Gaox Substramentioning

confidence: 99%

Galactose Oxidase/Prussian Blue Based Biosensors

et al. 2015

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“…274 More sensitive for H 2 O 2 (13.7 nM) were carbon screen-printed electrodes with osmiumwired horseradish peroxidase modifications. 275 Already in 2003, Xu et al presented H 2 O 2 sensors functionalized as colloidal gold modified screen-printed carbon electrodes. The enzyme horseradish peroxidase was additionally immobilized on the electrode, which then showed sensitivity for H 2 O 2 in the range of 400 to 800 nM.…”

Section: G87mentioning

confidence: 99%

Review—Quantification of Hydrogen Peroxide by Electrochemical Methods and Electron Spin Resonance Spectroscopy

Gulaboski

Mirčeski

Kappl

et al. 2019

J. Electrochem. Soc.

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Reactive oxygen species (ROS) exhibit different spatial and temporal distributions as well as concentrations in-and outside the cell, thereby functioning as signaling or pathogen-destroying molecules. Especially the ROS H 2 O 2 is important for the patho/physiological status of an organism. Electrochemistry (EM) and electron spin resonance (ESR)-based techniques allow quantification of H 2 O 2 in artificial and living systems, coping a concentration range from low nM up to mM. Working electrodes for EM are optimized by diverse modifications and, additionally, redox mediators are used. Ultramicroelectrodes allow scanning of single cells to spatially resolve and quantify extracellular H 2 O 2 in real-time. With ESR spectroscopy, • O 2¯, but not H 2 O 2 , can be directly determined by spin probes in-and outside of cells in suspensions. Monitoring H 2 O 2 requires formation of intermediate radicals, detectable with spin probes. Low μM [H 2 O 2 ] can thus be assessed specifically. Using suitable spin traps, in-vivo ESR and immuno-spin trapping can visualize different radicals at their respective production sites in small animals, organs and tissues. Here, the redox reaction cascades may interfere with cell metabolism. Optimization of all methods established for H 2 O 2 determination would be favorable to finally combine them for mutual validation. Thus, a deeper insight into cellular ROS metabolism can be obtained.

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“…Among the mediator based modified electrodes, HRP is the most commonly used material for the modification of the electrode in the last decade (Lin et al, 2000 ; Camacho et al, 2007 ; Radi et al, 2009 ; Ahammad, 2013 ). The sensitivity and selectivity of H 2 O 2 biosensor depends on the material used and modifications involved (Lin et al, 2000 ; Nakabayashi and Yoshikawa, 2000 ; Yao et al, 2005 ; Wang and Zhang, 2006 ; Camacho et al, 2007 ; Radi et al, 2009 ; Inoue et al, 2010 ; Li et al, 2013 ). The mechanism of detection of H 2 O 2 biosensor is described in Scheme 1 .…”

Section: Introductionmentioning

confidence: 99%

Detection of hydrogen peroxide in Photosystem II (PSII) using catalytic amperometric biosensor

et al. 2015

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Hydrogen peroxide (H2O2) is known to be generated in Photosystem II (PSII) via enzymatic and non-enzymatic pathways. Detection of H2O2 by different spectroscopic techniques has been explored, however its sensitive detection has always been a challenge in photosynthetic research. During the recent past, fluorescence probes such as Amplex Red (AR) has been used but is known to either lack specificity or limitation with respect to the minimum detection limit of H2O2. We have employed an electrochemical biosensor for real time monitoring of H2O2 generation at the level of sub-cellular organelles. The electrochemical biosensor comprises of counter electrode and working electrodes. The counter electrode is a platinum plate, while the working electrode is a mediator based catalytic amperometric biosensor device developed by the coating of a carbon electrode with osmium-horseradish peroxidase which acts as H2O2 detection sensor. In the current study, generation and kinetic behavior of H2O2 in PSII membranes have been studied under light illumination. Electrochemical detection of H2O2 using the catalytic amperometric biosensor device is claimed to serve as a promising technique for detection of H2O2 in photosynthetic cells and subcellular structures including PSII or thylakoid membranes. It can also provide a precise information on qualitative determination of H2O2 and thus can be widely used in photosynthetic research.

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Optimization of Hydrogen Peroxide Detection for a Methyl Mercaptan Biosensor

Cited by 20 publications

References 37 publications

Galactose Oxidase/Prussian Blue Based Biosensors

Galactose Oxidase/Prussian Blue Based Biosensors

Review—Quantification of Hydrogen Peroxide by Electrochemical Methods and Electron Spin Resonance Spectroscopy

Detection of hydrogen peroxide in Photosystem II (PSII) using catalytic amperometric biosensor

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