Optimization of in vitro and ex vitro regeneration and micromorphological studies of Micrococca mercurialis (L.) Benth.

Manokari, M.; Shekhawat, Mahipal S.

doi:10.17581/bp.2017.06105

Cited by 5 publications

(3 citation statements)

References 5 publications

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“…Nine shoots were produced by Lavia et al (1994) from the callus cultures of T. ulmifolia on MS medium fortified with NAA and BAP. The efficacy of BAP over other cytokinins for the establishment of cultures and multiplication of shoots has been reported by several authors for many plant species (Rathore et al, 2013;Cheruvathur et al, 2015;Lodha et al, 2015;Manokari and Shekhawat, 2017b). Zhang et al (2010) reported BAP as a better cytokinin for bud breaking and reinvigoration in Pinus radiata shoot cultures.…”

Section: Shoot Multiplication and Maintenance Of The Culturesmentioning

confidence: 83%

Improved micropropagation and foliar micromorphological studies in Turnera ulmifolia L. – An important medicinal plant

Manokari

Shekhawat²

2018

Folia Horticulturae

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The present study reports an efficient in vitro propagation system for Turnera ulmifolia using nodal segments as explants. Turnera ulmifolia (Passifloraceae) is an important garden plant with multipotent medicinal values. Effective shoot proliferation was achieved on agar gelled MS medium (Murashige and Skoog, 1962). The maximum number of shoots (8.3 ± 0.57) per initial explant was obtained on MS medium supplemented with 8.88 mM of 6-benzylaminopurine (BAP) and 0.54 mM of α-naphthalene acetic acid (NAA). The highest number of shoots (59.5 ± 2.10) proliferated on semi-solid MS medium (with agar) augmented with 2.22 mM of BAP and 2.32 mM of kinetin (Kin) along with 0.54 mM of NAA. Longer (4-5 cm) and healthy shoots were rooted (12.0 ± 0.10 roots per shoot) on half-strength MS medium fortified with 9.84 mM of indole-3 butyric acid (IBA). The in vitro regenerated plantlets were hardened in the greenhouse and transferred to the field. Significant developmental changes were observed in the foliar micromorphology of in vitro raised plantlets when these were transferred to the field. The stomatal index was gradually reduced (26.72 to 21.25) in the leaves from in vitro to field environments. But, vein-islets and veinlet terminations (13.4 and 7.6) were increased (39.7 and 18.4) respectively from in vitro to in vivo grown plants. Simple, unicellular, less frequent and underdeveloped trichomes were observed with the leaves of in vitro plants but fully developed trichomes recorded in the field transferred plants. The study could help in understanding the response and adaptation of tissue culture raised plantlets towards changed environmental conditions.

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Section: Shoot Multiplication and Maintenance Of The Culturesmentioning

confidence: 83%

Improved micropropagation and foliar micromorphological studies in Turnera ulmifolia L. – An important medicinal plant

Manokari

Shekhawat²

2018

Folia Horticulturae

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“…When producing Persian walnut in vitro explants, it is crucial to undergo hardening and acclimatization processes. This is due to the dysfunctional stomata of walnut in vitro-plantlets, resulting in excessive transpiration and heightened sensitivity to environments with increased evaporative demands and solar radiation (Chandra et al, 2010;Rathore et al, 2013;Manokari and Shekhawat, 2017). Plants are heterotrophic or mixotrophic under in vitro conditions, and their photosynthetic activity is limited (the carbon source is provided in the form of sugar).…”

Section: Discussionmentioning

confidence: 99%

Growth, photosynthetic function, and stomatal characteristics of Persian walnut explants in vitro under different light spectra

Saeedi,

Vahdati,

Sarikhani

et al. 2023

Front. Plant Sci.

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Light plays a crucial role in photosynthesis, which is an essential process for plantlets produced during in vitro tissue culture practices and ex vitro acclimatization. LED lights are an appropriate technology for in vitro lighting but their effect on propagation and photosynthesis under in vitro condition is not well understood. This study aimed to investigate the impact of different light spectra on growth, photosynthetic functionality, and stomatal characteristics of micropropagated shoots of Persian walnut (cv. Chandler). Tissue-cultured walnut nodal shoots were grown under different light qualities including white, blue, red, far-red, green, combination of red and blue (70:30), combination of red and far-red (70:30), and fluorescent light as the control. Results showed that the best growth and vegetative characteristics of in vitro explants of Persian walnut were achieved under combination of red and blue light. The biggest size of stomata was detected under white and blue lights. Red light stimulated stomatal closure, while stomatal opening was induced under blue and white lights. Although the red and far-red light spectra resulted in the formation of elongated explants with more lateral shoots and anthocyanin content, they significantly reduced the photosynthetic functionality. Highest soluble carbohydrate content and maximum quantum yield of photosystem II were detected in explants grown under blue and white light spectra. In conclusion, growing walnut explants under combination of red and blue lights leads to better growth, photosynthesis functionality, and the emergence of functional stomata in in vitro explants of Persian walnuts.

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“…ZnO-NPs can be synthesized in different crystal phases but the most stable one is hexagonal wurtzite structure [15]. Over the years, several methods for synthesizing ZnO-NPs including chemical reduction [16], thermal decomposition [17], photochemical reduction [18], electrochemical [19], hydrothermal [20], laser irradiation [21], pyrolysis [22], etc. have been applied, but limitations like toxicity of chemicals and harsh synthetic conditions are associated with these methods [23].…”

Section: Introductionmentioning

confidence: 99%

Biogenic synthesis of zinc oxide nanoparticles using leaves extract of Camellia sinensis for photocatalytic and biological applications

Sattar¹,

Rasool²,

Qadir³

et al. 2023

JOBM

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Nanoparticles have attracted considerable attention of researchers due to their diverse properties in the fields of catalysis, energy devices, wound healing and drug delivery systems . Synthesis of nanoparticles using plants and microbial extract is a green approach due to easy handling, rapidity and cost-effectiveness. This article reported a simple and green method of zinc oxide nanoparticles (ZnO-NPs) synthesis using Camellia sinensis leaves extract as reducing agent. State-of-the-art techniques were utilized for the characterization and measure the potential applications of ZnO-NPs. FTIR and SEM analysis were performed to confirm the nature of bonding and morphology of NPs. XRD analysis confirmed the hexagonal wurtzite structure and crystallite size (34 nm) of ZnONPs. EDX analysis was performed to check the purity of NPs. Energy band gap of valence band and conduction band was found 3.278 eV using UV/Visible spectrophotometry. Purified ZnO-NPs were utilized to determine the photocatalytic potential for degradation of hazardous dye (methylene blue) at λmax of 668 nm under irradiation of sunlight. The results indicated ∼92% photodecomposition of dye after 110 min of sunlight irradiation. Moreover, ZnO-NPs also revealed the antibacterial potential, having better inhibition power against gram-negative bacterial strains.

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Optimization of in vitro and ex vitro regeneration and micromorphological studies of Micrococca mercurialis (L.) Benth.

Cited by 5 publications

References 5 publications

Improved micropropagation and foliar micromorphological studies in Turnera ulmifolia L. – An important medicinal plant

Improved micropropagation and foliar micromorphological studies in Turnera ulmifolia L. – An important medicinal plant

Growth, photosynthetic function, and stomatal characteristics of Persian walnut explants in vitro under different light spectra

Biogenic synthesis of zinc oxide nanoparticles using leaves extract of Camellia sinensis for photocatalytic and biological applications

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