Optimization of in vitro cultivation strategies for human adipocyte derived stem cells

Abstract

With adipose-derived stem cells being in the focus of research in regenerative medicine, the need arises for fast reliable cultivation protocols. We have tested the cultivation of human adipose-derived stem cells in endothelial cell growth medium prior to induction and differentiation, against the long-established use of DMEM/F12 medium-based cultivation protocols. We found that cultivation in endothelial cell growth medium not only accelerates growth before induction and differentiation, but also allows shorter induction and differentiation times than those following precultivation with DMEM/F12 medium with regard to the formation of mature adipocytes and to the viability undifferentiated cells. These results were first observed morphologically but could be confirmed by performing adiponectin ELISA and cell proliferation assays.

The Influence of AGEs Environment on Proliferation, Apoptosis, Homeostasis, and Endothelial Cell Differentiation of Human Adipose Stem Cells

“…The differentiation potential also makes hASCs vulnerable to the external microenvironment. BSA in culture medium and trypsin neutralization solution, 18,19 cell contents from aged cell rupture, and apoptotic bodies can all affect hASCs homeostasis and differentiation; therefore, the whole culturing process used serum and antibiotics-free medium and low serum detach kit. The medium was changed every 2 days to remove dead and nonadherent cells.…”

Sulforaphene Suppresses Adipocyte Differentiation via Induction of Post-Translational Degradation of CCAAT/Enhancer Binding Protein Beta (C/EBPβ)

“…Thus, we discriminated HDM from MDM for efficient differentiation of ASCs. Instead of DMEM, we utilized DMEM-F12 which contains a wider variety of additional nutrients than DMEM and is the most commonly used medium to culture and to induce adipocyte differentiation of ASCs [37,38]. Additionally, in HDM 5 µg/mL more insulin was added in addition to 5 µg/mL insulin, 0.5 mM IBMX and 1 µM dexamethasone, the common three components for the induction of adipocyte differentiation.…”