Optimization of In Vitro Germination and storage of Armeniaca sibirica Pollen

Wu, Yong-Pei; Gao, Wenya; Zhou, Yuezhu; Guo, Huiyan

doi:10.1016/j.scienta.2022.111309

Cited by 4 publications

(1 citation statement)

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“…Pollen storage methods range from room temperature storage (25°C) to low-temperature storage in household refrigerators (4°C), freezers (−20°C), and deep freezers (−80°C), with options for storing fresh, desiccated, vacuum- or freeze-dried pollen. However, pollen can only be stored for short- to medium-term periods (hours to days to weeks to months) at these temperatures ( Fayos et al., 2022 ; Wu et al., 2022 ; Shankar et al., 2023 ). Cryopreservation is the storage of pollen at ultra-low temperature in liquid nitrogen (LN) either in the liquid phase (−196°C) or in the vapor phase (−150 to −180°C) retaining its original viability and offers long-term storage ( Rajasekharan and Rohini, 2023 ).…”

Section: Introductionmentioning

confidence: 99%

Simple cryopreservation protocol for Luffa pollen: enhancing breeding efficiency

Nagar,

Gowthami,

Sureja

et al. 2023

Front. Plant Sci.

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This study aimed to develop a long-term pollen storage protocol for Luffa species (L. acutangula, L. cylindrica, L. echinata, and L. graveolens) and assess its potential for crop improvement. The optimal medium for in vitro pollen germination varied by species, with Brewbaker and Kwack (BK) medium with 10% sucrose suitable for L. acutangula, L. cylindrica, and L. echinata, and BK medium with 3% sucrose ideal for L. graveolens. Overestimation in staining tests compared to in vitro pollen germination was observed. The best results for cryopreservation were achieved with desiccation periods of 20, 30, and 40 min, maintaining moisture content between 14.04% and 18.55%. Pollen viability was negatively correlated with storage temperature (25, 4, and −20°C) and duration. Cryopreserved pollen at −196°C exhibited the highest viability over a prolonged period (2 months) and was comparable to fresh pollen in terms of germination, ovule fertilization, and fruit and seed set. This study presents a simple and reproducible pollen cryopreservation protocol applicable across Luffa species, facilitating long-term storage and its use in crop improvement efforts.

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Section: Introductionmentioning

confidence: 99%

Simple cryopreservation protocol for Luffa pollen: enhancing breeding efficiency

Nagar,

Gowthami,

Sureja

et al. 2023

Front. Plant Sci.

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Effects of Vegetation Restoration Type on Soil Greenhouse Gas Emissions and Associated Microbial Regulation on the Loess Plateau

Zhou,

Liu,

et al. 2024

Ecology and Evolution

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Investigating responses of soil greenhouse gas (GHG) emissions to vegetation restoration is important for global warming mitigation. On the Loess Plateau, a wide range of vegetation restoration strategies have been implemented to control land degradation. However, the thorough quantification of soil GHG emissions triggered by different modes of vegetation restoration is insufficient. There is still a knowledge gap regarding the regulation of soil biochemical and microbial processing on soil GHG emissions. To do so, we compared responses of soil GHG emissions to various types of vegetation restoration on the Loess Plateau, and investigated the changes in soil properties as well as microbial composition and activities. We found that artificial plantation of Caragana korshinskii had low soil carbon dioxide (CO2) emission, while natural grassland had high CO2 emission. The possible explanations could be related to higher moisture and microbial biomass carbon, and greater nitrogen limitation in natural grassland, which was controlled by actinomycetes and gram‐negative bacteria. Natural grassland had low soil nitrous oxide (N2O) emission and high methane (CH4) uptake, whereas Prunus mume had high N2O emission and Medicago sativa had low CH4 uptake, respectively. Soil N2O emission could be driven by fungi and gram‐positive bacteria which were affected by N availability and dissolved organic carbon. Soil CH4 consumption was associated with anaerobic bacteria and gram‐negative bacteria which were affected by N availability and moisture. These different emissions of CO2, N2O and CH4 generated the largest total GHG emissions for plantation of Prunus mume, but the smallest total GHG emissions for natural grassland and plantation of leguminous Caragana korshinskii. Overall, our findings suggested that the restoration of natural grassland and artificial N‐fixing shrubland like Caragana korshinskii should be encouraged to alleviate GHG emissions, with the practical implications for selecting suitable modes and species to improve ecological sustainability in degraded lands.

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