Optimization of in vitro propagation of Coleonema album, a highly utilized medicinal and ornamental plant

ESTANDARIZACIÓN DE UN PROTOCOLO DE DESINFECCIÓN Y ESTABLECIMIENTO DE CULTIVO In vitro DEAloysia tryphilla

“…los mejores resultados fueron obtenidos con los protocolos 1, 2 y 3 con porcentajes de contaminación del 21,1 ± 6,9; 14,4 ± 5,1 y 10,0 ± 3,3%, respectivamente. los resultados obtenidos en los tratamientos 1 y 2 evidencian que la combinación de NaClO y Etanol al 70% presentan una gran alternativa en la desinfección de explantes [24]. No obstante, el alto grado de manipulación de explantes (Protocolo 1) y el alto tiempo de exposición a las soluciones utilizadas (Protocolo 2), favoreció la oxidación incrementando el número de explantes muertos (51,1 ± 8,4 y 33,3 ± 6,7%, respectivamente).…”

Large scale in vitro propagation of Anoectochilus roxburghii for commercial application: Pharmaceutically important and ornamental plant

“…Furthermore, with the increase in BA concentrations, the shoot lengths, shoot diameters and shoot fresh weights decreased. Likewise, the impact of BA on shoot bud initiation from nodal explants has been demonstrated in various plant species, such as Hibiscus cannabinus (Ayadi et al, 2011), Stevia rebaudiana (Thiyagarajan et al, 2012) and Coleonema album (Fajinmi et al, 2014). In present study, half-strength MS medium was fortified with 50 mg l −1 ascorbic acid, 25 mg l −1 citric acid, 25 mg l −1 adenine sulfate and 25 mg l −1 L-arginine.…”

Effective Surface Sterilization Method using Plant Preservatives Mixture and Shoot Multiplication of Clinacanthus nutans

“…Plant Preservative Mixture controls the growth of microbes by penetrating the microbial cell wall thus inhibiting several key enzymes in the citric acid cycle, this in turn disrupting the electron transport chain and inhibit the transport of monosaccharide and amino acids from media into microbial cells (George and Tripepi, 2001). Due to the complexity of plant cell wall, PPM molecules are reported not to be affecting the metabolic and transport pathways of plant tissues (George and Tripepi, 2001;Plant Cell Technology, 2019 (Namli et al, 2010) and Coleonema album (Fajinmi et al, 2014). Similar to KN, TDZ had no shoot promotional effect on C. nutans.…”