Optimization of in vitro regeneration protocol for a popular Indica rice (Oryza sativa L. cv Swarna)

Juturu, Vijaya; Mekala, Gopala Krishna; Mallikarjuna, G. B.; Reddy, Puli Chandra Obul; Sekhar, A. Chandra

doi:10.21746/aps.2016.08.002

Cited by 7 publications

(10 citation statements)

References 4 publications

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“…Our results are in agreement with those reported by Sah et al (2014) who demonstrated the advantage of phytagel over agar for callus induction frequency in rice (using the Kitaake cultivar). Juturu et al (2016) also found that medium solidified with phytagel gave 30% higher callus fresh weight using the Swarna rice cultivar. In addition to phytagel, gelrite was found to show higher efficiency for callus induction as compared to agar using Swarna and Mahsuri rice cultivars ( Jadhav et al, 2011 ).…”

Section: Discussionmentioning

confidence: 87%

Effects of different gelling agents on the different stages of rice regeneration in two rice cultivars

Mohamed

Amer

Osman

et al. 2021

Saudi Journal of Biological Sciences

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Plant tissue culture technology offers a solution for meeting the increasing commercial demand on economically important plants such as rice, a widespread dietary staple. However, significant genotype-specific morphogenetic responses constitute a considerable on rice regeneration in plant biotechnology contexts. Aside from genotype dependency, the components of the nutrient media including gelling agents have an important impact on regeneration efficiency. The current study explores the effect of different gelling agents on various stages of rice regeneration in two Egyptian rice cultivars-Sakha104 and Giza178. Media solidified with varying concentrations of a variety of gelling agents (agar, bacto agar, gelrite and phytagel) were tested for their impact on the frequency of callus induction, shoot regeneration and rooting. The results indicated gellan gum (gelrite and phytagel) was superior to agar products (agar and bacto agar) for callus induction. By contrast, no significant differences were found between different gelling agents for shoot regeneration. Gellan gum and media solidified with bacto agar were found to lead to significantly higher root regeneration than agar. The Sakha104 cultivar showed better responses than Giza 178 for callus induction and similar performance to the Giza 178 cultivar for root regeneration irrespective of the gelling agent. This work provides insights into the impact of different gelling agents on the morphogenetic response of two rice cultivars and can be used to help maximize the frequency of rice regeneration.

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Section: Discussionmentioning

confidence: 87%

Effects of different gelling agents on the different stages of rice regeneration in two rice cultivars

Mohamed

Amer

Osman

et al. 2021

Saudi Journal of Biological Sciences

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“…High percentage of re-generable embryogenic calli is a prerequisite for genetic manipulation towards varietal improvement [55]. This study aimed to test and improve the embryogenic potential of calli for better plant regeneration efficiency.…”

Section: Callus Inductionmentioning

confidence: 99%

Efficient Callus Induction and Regeneration in Selected Indica Rice

Mostafiz

Wagiran

2018

Agronomy

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An efficient callus induction and in vitro regeneration were developed using plant growth regulators, carbon sources, and basal media for three selected Malaysian wetland rice varieties (MR220, MR220-CL2, and MR232) and one upland variety (Bario). Effect of plant growth regulator (PGR) was carried out using four different concentrations (1-4 mg/L) of 2,4-D (2, 4-dichlorophenoxyacetic acid), and NAA (1-naphthalene acetic acid) (2.5, 5.0, 7.5, and 10 mg/L) with optimized 2,4-D. Effects of carbon sources (maltose and sorbitol), and basal media (MS, N6, and LS) were also studied with optimized PGR to maximize the induction of regenerable calli. This study found that all four varieties exhibited high frequency of callus induction on MS (Murashige and Skoog) medium that was supplemented with 3 mg/L 2,4-D and 30 g/L maltose. Callus induction frequencies in the cases of MR220, MR220-CL2, MR232, and Bario were found to be 76%, 94%, 85%, and 42% respectively. Morphological analysis through scanning electron microscopy (SEM) and histological analysis revealed the embryogenicity of the induced callus. In the regeneration study, it was observed that combination of 2 mg/L BAP (6-benzylaminopurine), 2 mg/L Kin (Kinetin) and 0.5 mg/L NAA supplemented MS medium has the potential to promote regeneration of selected indica rice varieties with higher regeneration percentage, i.e., 82% (MR220-CL2), 68% (both in MR220 and MR232), and 40% (Bario). The optimized conditions for callus formation and regeneration can be useful for biotechnological practices for the genetic improvement of Malaysian indica rice.

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“…The high percentage of re-generable embryogenic calli is a prerequisite for genetic manipulation towards varietal improvement. This study aimed to test and improve the embryogenic potential of calli for better plant regeneration e ciency (Juturu et al 2016). Flower and boll number is an important trait for upland cotton.…”

Section: Discussionmentioning

confidence: 99%

“…The highest callus induction was observed in shoot tip tissue and this is possibly due to the presence of meristematic cells at the tip end of the seedling in Gossypium hirsutum. It was reported that high percentage of re-generable embryogenic calli is a prerequisite for genetic manipulation towards varietal improvement (Juturu et al, 2016). Embryogenic callus is best requirement for successful tissues regeneration.…”

Section: Discussionmentioning

confidence: 99%

Validating Field Regeneration Capacity for Selected Accessions of Gossypium hirsutum Using Callus Induction and Regeneration Capacity

Tajo¹,

Pan²,

Sadau³

et al. 2021

Preprint

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Gossypium hirsutm undergoes a rapid clonal propagation to regenerate a mature plant through tissue culture. In this research, cotton leaf regeneration level for 21 accessions in the field (new leaves) was observed after the first harvest, and a comparison between field regeneration level and callus induction with its regeneration capacity (new shoots and roots) for the same 21 accessions was carried out. During the flowering stage of Gossypium hirsutum, biochemical (Proline), physiological (chlorophyll and carotenoid content) analysis was carried out. Phenotypic observations (plant height, leaf area, fresh leaf weight, dry leaf weight, flower and boll number) were also carried out on 21 accessions for each accession. Callus induction and regeneration capacity of roots and shoots for hypocotyl, cotyledons and shoot tip tissues was used to validate field regeneration capacity through analysis of variance. ZS061, LuMian378, JiMian863, and ZS065 have highest drought tolerance while ZhongMianSuo24, LiaoYangDuoMaoMian, and BeiZheGongSheMian have the lowest tolerance to drought stress. Accessions with both field and callus regeneration capacity were identified.

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Optimization of in vitro regeneration protocol for a popular Indica rice (Oryza sativa L. cv Swarna)

Cited by 7 publications

References 4 publications

Effects of different gelling agents on the different stages of rice regeneration in two rice cultivars

Effects of different gelling agents on the different stages of rice regeneration in two rice cultivars

Efficient Callus Induction and Regeneration in Selected Indica Rice

Validating Field Regeneration Capacity for Selected Accessions of Gossypium hirsutum Using Callus Induction and Regeneration Capacity

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