Optimization of Lipase Purification by Rotating Annular Size-exclusion Chromatography

Vasudevan, Palligarnai T.; Palekar, Ashish A.; Yan, Shuo

doi:10.1080/10242420290020714

Cited by 4 publications

(5 citation statements)

References 8 publications

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“…A decrease in flow rate leads to an increase in the solute band width due to eddy diffusion. This has been observed in practice [52].…”

Section: Effect Of Flow Ratesupporting

confidence: 48%

“…The major advantage of using such a purification technique, according to Genest et al, is the higher rate of lipase purification and the ease of scale-up. Vasudevan et al [52] optimized the purification process in a rotating annular size-exclusion chromatography unit. The parameters that were optimized for efficient functioning of the column included mobile phase flow rate, lipase flow rate, introduction of multiple feeds to maximize throughput of the column and rotation rate.…”

Section: Unique Purification Strategiesmentioning

confidence: 99%

“…The author also acknowledges Taylor and Francis (www.tandf.co.uk) for granting permission to use material from two articles published by the author [2,52].…”

Section: Acknowledgementsmentioning

confidence: 99%

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Purification of Lipase

Vasudevan

2004

Lipases and Phospholipases in Drug Development

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Abbreviations a hydrodynamic radius of the solute (m) C m mobile phase concentration (mol l -1 ) C f feed concentration (mol l -1 ) C s stationary phase concentration (mol l -1 ) C H m normalized mobile phase concentration C H s normalized stationary phase concentration C H m normalized mobile phase concentration in the Laplace domain C H s normalized stationary phase concentration in the Laplace domain C D drag coefficient D m dispersion coefficient, m 2 s -1 D s diffusion coefficient in the stationary phase, m 2 s -1 D ? bulk diffusivity, m 2 s -1 k mass transfer coefficient, m s -1 K eq equilibrium partition coefficient L length of the column, m m n n th moment about the origin N Avogadro number Nu s Nusselt number in the stationary phase Nu m Nusselt number in the mobile phase Pe Peclet number R particle size, m r 0 pore radius, m Re Reynolds number S Particle surface area per unit column volume, m -1 Sh Sherwood number Sc Schmidt numberGreek letters b internal porosity e external porosity m kinematic viscosity, m 2 s -1

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“…A decrease in flow rate leads to an increase in the solute band width due to eddy diffusion. This has been observed in practice [52].…”

Section: Effect Of Flow Ratesupporting

confidence: 48%

Section: Unique Purification Strategiesmentioning

confidence: 99%

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Purification of Lipase

Vasudevan

2004

Lipases and Phospholipases in Drug Development

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“…A continuous rotating annular size-exclusion chromatography system permitted the purifi cation of crude porcine lipase with productivity of approximately 3 mg lipase per mg gel per hour and an activity recovery of almost 99 % [ 8 ].…”

Section: Column Cleaning and Storagementioning

confidence: 99%

Gel-Filtration Chromatography

Ó’Fágáin

Cummins

O’Connor

2016

Methods in Molecular Biology

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“…with productivity of approximately 3 mg lipase per mg gel per hour and an activity recovery of almost 99% (6). Among fooduse proteins, hen egg lysozyme has been successfully refolded using both acrylamide-and dextran-based gel columns (Sephacryl S-100 and Superdex 75, respectively) (7).…”

Section: Separation Of Proteins and Peptidesmentioning

confidence: 99%