Optimization of lovastatin production from Aspergillus fumigatus

Abstract

The present investigation, focused on screening of various fungal species for Lovastatin production using different agro-based wastes, also, for maximizing lovastatin productivity by isolated Aspergillus fumigatus using response surface methodology (RSM). The following substrates (Olive cake; Pea pods; sugarcane bagasse; wheat bran; rice hulls; beet peel; Potato peel and groundnut shells) were screened to evaluate their effectiveness for lovastatin production, using different fungal species, (Aspergillus niger; Rhizopus oligosporus; Penicillium citrinum and isolated Aspergillus fumigatus) under solid state fermentation (SSF). Wheat bran was the most suitable substrate for lovastatin production with all fungal species. Optimum conditions of lovastatin production by wheat bran have been attained efficiently by response surface methodology (RSM) using isolated Aspergillus fumigatus under solid state fermentation (SSF). The lovastatin yield of (3.353 mg/g DFM) was obtained at an optimum temperature of 28 °C; pH of 5.00; initial moisture content of 70% and incubation period of 12 days. This Lovastatin has the possibility to use in different therapeutic applications.

Optimization of the Production of Lovastatin from Aspergillus sclerotiorum PSU- RSPG178 under Static Liquid Culture using Response Surface Methodology

“…Consequently, it can be concluded that temperature exerted a significant effect on lovastatin production. This may be due to the fact that increasing the temperature leads to poor heat dissipation and a reduction in the oxygen level, thereby inhibiting the growth of microorganisms (Mouafi et al 2016), which is in agreement with Negishi et al (1986) and Munir et al (2018), who found that increasing the temperature to 35°C inhibited lovastatin production because the metabolic processes of denaturing enzymes and other proteins were retarded.…”

“…The fungi used in the study, with their yields, were as follows: A. terreus (55 mg/L), A. parasiticus (4.5 mg/L), A. fischeri (2.0 mg/L), A. flavus (9.0 mg/L), A. umbrosus (14.1 mg/L), Penicillium funiculosom (pinophilum)(19.3 mg/L), Trichoderma viridae (9.0 mg/L), Trichoderma longibrachiatum (1.0 mg/L), Acremonium chrysogenum (2.5 mg/L). Mouafi et al (2016) reported that the optimal lovastatin production (3.353 mg/g of dried fermented matter) from A. fumigatus was achieved at a temperature of ~28°C and a pH5.0 with an incubation period of 12 days. Elsewhere, the optimal yield of lovastatin from Monascus ruber, incubated at 25°C, at pH5.0 under submerged fermentation with shaking at 150 rpm for 10 days, was 131 mg/L (Sripalakit et al 2011).…”

Bioprocessing of Two Crop Residues for Animal Feeding into a High-Yield Lovastatin Feed Supplement

“…Spores were counted with a hemocytometer and adjusted to 1 × 10 7 spores/mL. Finally, 2 mL of each suspension was utilized as the inoculum [ 29 ].…”

Optimization of process variables for hyper-production of lovastatin from wild type Aspergillus terreus and its efficacy studies

“…Bizukojc et al, (2012) reported that neutral and basic pH range produced greater lovastatin formation as acidic ones This is due to effect for polyketide pathways (A. terreus) and the substantial reduce of (+) geodin (Bizukojc and Ledakowicz, 2008). pH also influences lovastatin production (Bizukojc et al, 2012) as pH powerfully effects the transportation of many ingredients through the physiological membrane (Mouafi et al, 2016). Inoculum size in SSF has been studied positive and negative influences.…”