Optimization of medium components for hyaluronic acid production by Streptococcus zooepidemicus MTCC 3523 using a statistical approach

Patil, Kanchankumar P.; Kamalja, Kirtee K.; Chaudhari, Bhushan L.

doi:10.1016/j.carbpol.2011.06.065

Cited by 21 publications

(6 citation statements)

References 23 publications

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“…thermophilus needs to reach a high level. In accordance with previous studies, − we planned to engineer S. thermophilus as shown in Figure a to improve HA production.…”

Section: Resultsmentioning

confidence: 71%

Enhanced Stability and Function of Probiotic Streptococcus thermophilus with Self-Encapsulation by Increasing the Biosynthesis of Hyaluronan

Zhou

et al. 2022

ACS Appl. Mater. Interfaces

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The harsh conditions of the gastrointestinal tract limit the potential health benefits of oral probiotics. It is promising that oral bioavailability is improved by strengthening the selfprotection of probiotics. Here, we report the encapsulation of a probiotic strain by endogenous production of hyaluronan to enhance the effects of oral administration of the strain. The traditional probiotic Streptococcus thermophilus was engineered to produce hyaluronan shells by using traceless genetic modifications and clustered regularly interspaced short palindromic repeat interference. After oral delivery to mice in the form of fermented milk, hyaluronan-coated S. thermophilus (204.45 mg/L hyaluronan in the milk) exhibited greater survival and longer colonization time in the gut than the wild-type strain. In particular, the engineered probiotic strain could also produce hyaluronan after intestinal colonization. Importantly, S. thermophilus self-encapsulated with hyaluronan increased the number of goblet cells, mucus production, and abundance of the microorganisms related to the biosynthesis of short-chain fatty acids, resulting in the enhancement of the intestinal barrier. The coating formed by endogenous hyaluronan provides an ideal reference for the effective oral administration of probiotics.

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“…thermophilus needs to reach a high level. In accordance with previous studies, − we planned to engineer S. thermophilus as shown in Figure a to improve HA production.…”

Section: Resultsmentioning

confidence: 71%

Enhanced Stability and Function of Probiotic Streptococcus thermophilus with Self-Encapsulation by Increasing the Biosynthesis of Hyaluronan

Zhou

et al. 2022

ACS Appl. Mater. Interfaces

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“…The Gw and S. zooepidemicus (as a control strain without undergoing mutation) were incubated in BHI broth at 37 °C for 24 h. The pellets were then inoculated into the production medium (1.5 mg/ml of Na 2 HPO 4 .12H 2 O, 1.5 mg/ml of KH 2 PO 4 , 0.5 mg/ml of MgSO 4 .7H 2 O, 10 mg/ml of yeast extract, and 20 mg/ml of glucose) and incubated at different cultivation periods (4, 8, and 18 h) at 37 °C/220 rpm. The components of the production medium were justified for HA production according to previous studies [ 13 , 17 , 18 ] . The optimum pH of production reported ranging from 6.9 to 7.4 for GCS [ 17 , 19 , 20 ] , whereas, in our study, a broader pH range (4.5, 5.0, 5.5, and 7.0) was investigated in comparison to S. zooepidemicus .…”

Section: Methodsmentioning

confidence: 99%

Development of Streptococcus equisimilis Group G Mutant Strains with Ability to Produce Low Polydisperse and Low-Molecular-Weight Hyaluronic Acid

Jafari

Keramati

Cohan

et al. 2022

IBJ

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Background: Hyaluronic acid, a natural polymer with wide applications in biomedicine and cosmetics, is mainly produced by Streptococcal fermentation at industrial scale. In the present study, chemical random mutagenesis was used for development of Streptococcus equisimilis group G mutant strains with high HA productivity. Methods: The optimum of the pH of culture condition and cultivation time for HA production by wild strain group G were assessed. At first, two rounds of mutation at different concentrations of NTG was used for mutagenesis. Then, the nonhemolytic and hyaluronidase-negative mutants were screened on the blood and HA agar. HA productivity and molecular weight were determined by carbazole assay, agarose gel electrophoresis and specific staining. Moreover, stability of the high producer mutants was evaluated within 10 generations. Results:The results showed that the wild-type strain produced 1241 ± 2.1 µg/ml of HA at pH 5.5 and 4 hours of cultivation, while the screened mutants showed a 16.1-45.5% increase in HA production. Two mutant strains, named Gm2-120-21-3 (2470 ± 8.1 µg/ml) and Gm2-120-21-4 (2856 ± 4.2 µg/ml), indicated the highest titer and a consistent production. The Mw of HA for the mutants was less than 160 kDa, considering as a low Mw HA. Conclusion:The mutant strains producing a low polydisperse, as well as low Mw of HA with high titer might be regarded as potential industrial strains for HA production after further safety investigations.

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“…Optimization of medium for production was using Taguchi orthogonal array design and 24 full factorial designs was reported earlier by Im et al, (2009) andPatil et al, (2011), respectively. In above studies, higher average HA production was observed using high level( 4.5 %) of glucose, high level ( 4.5 %) of soya peptone, low level (0.075%) of MgSO4.7H2O and high level (0.25 %)of K2HPO4.Soyapeptone(X2) had a significant effect (P=0.005) on HA yield.…”

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confidence: 99%

Effect of Various Physical Parameters and Statistical Medium Optimization on Production of Hyaluronic Acid Using S. Equi Subsp. Zooepidemicus ATCC 39920

Aroskar¹,

Kamat²,

Kamat³

2013

iioablett

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It has been shown that initial conditions for bacterial cultivation are extremely important for the successful production of hyaluronic acid (HA) by fermentation. We investigated several physical parameters that affect productivity of HA under shake flask. i.e. transfer criteria of seed, agitation and aeration of fermentation flasks. Among the various physical parameters studied, inoculum age of 8-10 h, pH 6.4, optical density (600 nm) 2.0 and 3% level inoculum transfer found to be optimum. After inoculating with Streptococcus equi subsp. zooepidemicus ATCC 39920, the temperature 37 0 C and 90 rpm found optimum during growth as well as for the HA production. The fractional factorial design of six factors with two levels showed yeast extract, potassium dihydrogen phosphate and sodium bicarbonate as significant model terms. The factor potassium dihydrogen phosphate was relatively more significant than yeast extract.

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Optimization of medium components for hyaluronic acid production by Streptococcus zooepidemicus MTCC 3523 using a statistical approach

Cited by 21 publications

References 23 publications

Enhanced Stability and Function of Probiotic Streptococcus thermophilus with Self-Encapsulation by Increasing the Biosynthesis of Hyaluronan

Enhanced Stability and Function of Probiotic Streptococcus thermophilus with Self-Encapsulation by Increasing the Biosynthesis of Hyaluronan

Development of Streptococcus equisimilis Group G Mutant Strains with Ability to Produce Low Polydisperse and Low-Molecular-Weight Hyaluronic Acid

Effect of Various Physical Parameters and Statistical Medium Optimization on Production of Hyaluronic Acid Using S. Equi Subsp. Zooepidemicus ATCC 39920

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