Optimization of medium constituents for improved chitinase production by<i>Paenibacillus</i>sp. D1 using statistical approach

Singh, Anil Kumar; Mehta, Gunjan; Chhatpar, H. S.

doi:10.1111/j.1472-765x.2009.02731.x

Cited by 42 publications

(31 citation statements)

References 16 publications

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“…The unoptimized medium used for growth and protease production was as described earlier (Singh et al 2009) except that carbon (chitin) and nitrogen ((NH 4 ) 2 SO 4 ) source was replaced by protein source soybean meal, 10 g l -1 , pH 7.2. The organism was grown at 30°C under shaking conditions (180 rpm) for 72 h. The media components and their composition for protease production during optimization study are given in Tables 1 and 2.…”

Section: Organism and Culture Conditionsmentioning

confidence: 99%

Optimization of protease production by Streptomyces sp. A6 using statistical approach for reclamation of shellfish waste

Singh

Chhatpar

2010

World J Microbiol Biotechnol

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Protease producing Streptomyces sp. A6 was isolated from intertidal zone of the coast of Diu (Gujarat, India). Plackett-Burman method was applied to identify important factors (shrimp waste, FeCl 3 , ZnSO 4 and pH) influencing protease production by Streptomyces sp. A6. Further optimization was done by response surface methodology using central composite design. The concentrations of medium components for higher protease production as optimized using the above approach were (g l -1 ): Shrimp waste, 14; FeCl 3 , 0.035; ZnSO 4 , 0.065 and pH, 8.0. This statistical optimization approach led to production of 129.02 ± 2.03 U ml -1 of protease which was 4.96 fold higher compared to that obtained using the unoptimized medium. The protease production was scaled to 3 l in a 5-l bench fermenter using optimized medium which further increased the production by 63.4%. Deproteinization and chitin recovery obtained at the end of fermentation was 85.12 ± 4.7 and 70.58 ± 1.33%, respectively. The present study is the first report on statistical optimization of medium components for production of protease by Streptomyces species using cheaper raw material such as shrimp waste. The study also explored the possibility Streptomyces sp. A6 for reclamation of shrimp wastes.

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Section: Organism and Culture Conditionsmentioning

confidence: 99%

Optimization of protease production by Streptomyces sp. A6 using statistical approach for reclamation of shellfish waste

Singh

Chhatpar

2010

World J Microbiol Biotechnol

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“…Yeast extract source had a significant effect on chitinase production. [26,33,34] Shrimp shell waste containing chitin source was reported to be an important factor for chitinase production. [35] Gohel et al [36] has reported CaCl 2 as an influential media constituent for chitinase production by Pantoea dispersa.…”

Section: Selection Of Important Medium Components Using Plackettàburmmentioning

confidence: 99%

“…[24] This design is useful in screening studies that uses only k C 1 treatment combinations to estimate the main effects of k factors independently, assuming that all interactions are insignificant. [23,25,26] A saturated design is used in the early stages of scientific tests in order to sort out unimportant factors from a large number of possible factors. When the number of factors in full factorial designs increases, the number of experiments becomes unmanageable.…”

Section: Introductionmentioning

confidence: 99%

Statistical optimization of medium components for chitinase production byPseudomonas fluorescensstrain HN1205: role of chitinase on egg hatching inhibition of root-knot nematode

Lee

Kim

2015

Biotechnology & Biotechnological Equipment

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In the present research, statistical PlackettÀBurman design (PBD) and central composite design (CCD) were used to optimize the medium's components in order to enhance the chitinase activity of Pseudomonas fluorescens strain HN1205. Among the seven nutritional elements that were studied, crab shell powder, CaCl 2 ¢2H 2 O and yeast extract were proved using PBD to have significant effect on chitinase activity. An optimal medium was obtained by applying a three-factor central composite design, which consisted of crab shell powder (1.0 g/L), CaCl 2 (0.5 g/L) and yeast extract (0.5 g/L), with the highest chitinase activity of 1.03 U/mL. This value was 2.87-fold higher than the activity obtained in the lowest productive medium in the design matrix. The chitinase produced by the HN1205 strain was partially purified with 80% ammonium sulphate and anion-exchange chromatography and assessed for inhibition of hatching of nematode eggs. The partially purified chitinase significantly reduced the hatch of Meloidogyne incognita eggs (8.1%), as compared to the effect of 10 mmol/L Tris-HCl buffer (pH 8.0) (49.4%) and boiled chitinase (54.7%). This study demonstrated the role of chitinolytic enzymes produced by P. fluorescens strain HN1205. The obtained optimal activity proved that the enzymes could be potential biological control agents.

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“…4). The results for the reported chitinase production varied between 0.1 and more than 60 U/ml (Bhushan 2000;Vaidya et al 2001;Singh et al 2009;Souza et al 2009). These variations were observed because of the strains and the level of enzyme purification.…”

Section: Runsmentioning

confidence: 99%

A novel halo-alkali-tolerant and thermo-tolerant chitinase from Pseudoalteromonas sp. DC14 isolated from the Caspian Sea

Makhdoumi

Dehghani-Joybari

Mashreghi

et al. 2015

Int. J. Environ. Sci. Technol.

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A novel halo-alkali and thermo-tolerant chitinase was obtained from an isolated strain found in the Caspian Sea. The effects of media composition and various fermentation conditions for the optimization of chitinase production were studied one factor at a time and by response surface methodology. The novel strain, which is designated as strain DC14 and phylogenetically related to the genus Pseudoalteromonas, produced chitinase after 72 h under the following optimal conditions: glucose 1 % (w/v), ammonium sulphate 0.2 % (w/v), chitin 1.07 % (w/ v), pH 8, NaCl 10 % (w/v), inoculums size 2.5 % (v/v), temperature 30°C, CaCl 2 3 mM and MgCl 2 4 mM. Using the statistical optimization method, chitinase production was found to increase from 2.30 to 21.90 U/dl. The enzyme showed maximum activity at 40°C, pH 9 and 10 % NaCl. It was stable in a wide range of temperature from 15 to 65°C, pH from pH 7 to 11 and NaCl concentration from 0 to 15 % (w/v). The molecular weight of the enzyme was estimated by SDS-PAGE to be about 65 kDa. With regard to the halo-alkali and thermo-stable properties of this enzyme, it has potential industrial activity.

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Optimization of medium constituents for improved chitinase production byPaenibacillussp. D1 using statistical approach

Cited by 42 publications

References 16 publications

Optimization of protease production by Streptomyces sp. A6 using statistical approach for reclamation of shellfish waste

Optimization of protease production by Streptomyces sp. A6 using statistical approach for reclamation of shellfish waste

Statistical optimization of medium components for chitinase production byPseudomonas fluorescensstrain HN1205: role of chitinase on egg hatching inhibition of root-knot nematode

A novel halo-alkali-tolerant and thermo-tolerant chitinase from Pseudoalteromonas sp. DC14 isolated from the Caspian Sea

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