Optimization of Method to Extract Collagen from “Emperor” Tissue of Soft-shelled Turtles

Yamamoto, Tetsushi; Uemura, Kentaro; Sawashi, Yuki; Mitamura, Kuniko; Taga, Atsushi

doi:10.5650/jos.ess15220

Cited by 6 publications

(8 citation statements)

References 24 publications

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“…Collagen extraction was performed in accordance with the our previous study . Briefly, emperor tissue was treated with 0.1 M formic acid at a ratio of 1:10 (w/v) for 24 h for demineralization.…”

Section: Methodsmentioning

confidence: 99%

“…Collagen extraction was performed in accordance with the our previous study. 26 Briefly, emperor tissue was treated with 0.1M formic acid at a ratio of 1:10 (w/v) for 24 h for demineralization. The sample was then treated with 0.1M sodium hydroxide (NaOH) at a ratio of 1:10 (w/v) for 3 days to remove noncollagenous proteins, including endogenous proteases.…”

Section: Collagen Extractionmentioning

confidence: 99%

“…Therefore, collagen of marine origin, such as fish, sponges, and mollusks, was recently considered as a useful alternative to mammalian sources because of its high availability . In addition, we previously reported that soft‐shelled turtle ( Pelodiscus sinensis ) tissue could be a useful alternative for collagen . Recently, several reports demonstrated its usefulness, making collagen from soft‐shelled turtle a useful material for cosmetics, pharmaceuticals, and medical care products.…”

Section: Introductionmentioning

confidence: 99%

“…[18][19][20][21][22][23][24][25] In addition, we previously reported that soft-shelled turtle (Pelodiscus sinensis) tissue could be a useful alternative for collagen. 26 Recently, several reports demonstrated its usefulness, 27,28 making collagen from soft-shelled turtle a useful material for cosmetics, pharmaceuticals, and medical care products.…”

Section: Introductionmentioning

confidence: 99%

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Shotgun label‐free proteomic analysis for identification of proteins in HaCaT human skin keratinocytes regulated by the administration of collagen from soft‐shelled turtle

et al. 2017

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Soft‐shelled turtles (Pelodiscus sinensis) are widely distributed in some Asian countries, and we previously reported that soft‐shelled turtle tissue could be a useful material for collagen. In the present study, we performed shotgun liquid chromatography (LC)/mass spectrometry (MS)‐based global proteomic analysis of collagen‐administered human keratinocytes to examine the functional effects of collagen from soft‐shelled turtle on human skin. Using a semiquantitative method based on spectral counting, we were able to successfully identify 187 proteins with expression levels that were changed more than twofold by the administration of collagen from soft‐shelled turtle. Based on Gene Ontology analysis, the functions of these proteins closely correlated with cell–cell adhesion. In addition, epithelial–mesenchymal transition was induced by the administration of collagen from soft‐shelled turtle through the down‐regulation of E‐cadherin expression. Moreover, collagen‐administered keratinocytes significantly facilitated wound healing compared with nontreated cells in an in vitro scratch wound healing assay. These findings suggest that collagen from soft‐shelled turtle provides significant benefits for skin wound healing and may be a useful material for pharmaceuticals and medical care products. © 2017 The Authors Journal of Biomedical Materials Research Part B: Applied Biomaterials Published by Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 2403–2413, 2018.

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Section: Methodsmentioning

confidence: 99%

Section: Collagen Extractionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Shotgun label‐free proteomic analysis for identification of proteins in HaCaT human skin keratinocytes regulated by the administration of collagen from soft‐shelled turtle

et al. 2017

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“…The aquatic processing industry generates large quantities of underutilized waste (approximately 30–70%), including bone frames, viscera, heads, and skins, etc., which become an enormous waste of resources and source of environmental pollution . Soft‐shelled turtle ( Pelodiscus sinensis ) is a commercially important aquatic species in China, Japan, and Southwest Asia, etc., due to its delicious taste, its high nutritional (high protein and low fat) and medicinal value (decreasing blood pressure, anti‐aging, anti‐fatigue, and helping to enhance immunity) . In China, there is a large market for soft‐shelled turtle, yielding more than 350 000 tons annually .…”

Section: Introductionmentioning

confidence: 99%

Bioavailability and prebiotic potential of Carapax Trionycis, a waste from soft‐shelled turtle processing

Wang

Chen

et al. 2020

J Sci Food Agric

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BACKGROUND Carapax Trionycis is the shell of the soft‐shelled turtle. It is rich in minerals, amino acid, peptides, and other nutrients. Current processing and consumption of soft‐shelled turtle leads to the waste of huge quantities of Carapax Trionycis in the form of spent materials. In this study, the bioavailability, prebiotic activity, and physicochemical properties of Carapax Trionycis using different processing methods were investigated. The vinegar‐quenched Carapax Trionycis (V‐CT), fine powders (D0.18, D0.10), and superfine powders (D0.05, D0.025) of Carapax Trionycis were prepared by the vinegar‐quenching method, common grinding, and the superfine grinding method. RESULTS The average particle sizes of D0.18, D0.10, D0.05, and D0.025 were 147.82, 77.35, 36.65, and 2.24 μm, respectively. Superfine grinding changed the surface morphology of Carapax Trionycis and promoted the release of active ingredients. D0.025 had the highest polypeptide (8.15%), polysaccharide (1.21%), total free amino acid (232.36 mg 100 g−1) and water‐soluble extract content (10.74%), and showed the highest calcium release rate (55.64%) after in vitro digestion. The apparent permeability (PAPP) of the resulting Carapax Trionycis samples in the dialysis tubing model and the everted intestinal sac model increased significantly with the decrease in the Carapax Trionycis particle sizes. Furthermore, the five Carapax Trionycis samples significantly stimulated the growth of the tested probiotics and increased lactic acids production after 48 h fermentation compared to the control. The Carapax Trionycis powder prepared by superfine grinding displayed better prebiotic activity than other samples as it significantly induced a greater proliferation of probiotic bacteria and higher production of lactic acid, as well as greater release of free calcium. CONCLUSIONS The results showed that Carapax Trionycis superfine powder D0.025 had the highest active ingredient content, calcium bioavailability, and prebiotic activity. Our approach of developing Carapax Trionycis superfine powder as natural calcium supplement or potential prebiotic would therefore broaden the scope of soft‐shelled turtle processing waste utilization in an eco‐friendly, cost‐effective, and sustainable approach in the future. © 2020 Society of Chemical Industry

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Identification and application of species-specific peptide biomarkers from soft-shelled turtles (Pelodiscus sinensis) using post-translational modification detection-based peptidomics analysis

et al. 2023

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Optimization of Method to Extract Collagen from “Emperor” Tissue of Soft-shelled Turtles

Cited by 6 publications

References 24 publications

Shotgun label‐free proteomic analysis for identification of proteins in HaCaT human skin keratinocytes regulated by the administration of collagen from soft‐shelled turtle

Shotgun label‐free proteomic analysis for identification of proteins in HaCaT human skin keratinocytes regulated by the administration of collagen from soft‐shelled turtle

Bioavailability and prebiotic potential of Carapax Trionycis, a waste from soft‐shelled turtle processing

Identification and application of species-specific peptide biomarkers from soft-shelled turtles (Pelodiscus sinensis) using post-translational modification detection-based peptidomics analysis

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