Optimization of methods to assess human mucosal T-cell responses to HIV infection

Shacklett, Barbara L.; Yang, Otto O.; Hausner, Mary Ann; Elliott, Julie; Hultin, Lance E.; Price, Charles; Fuerst, Marie; Matud, Jose L.; Hultin, Patricia M.; Cox, Catherine A.; Ibarrondo, Javier; Wong, Johnson T.; Nixon, Douglas F.; Anton, Peter A.; Jamieson, Beth D.

doi:10.1016/s0022-1759(03)00255-2

Cited by 95 publications

(111 citation statements)

References 51 publications

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“…Lamina propria lymphocytes (LPLs) were isolated from antral biopsy specimens by collagenase type II (Sigma) digestion (16). Samples were incubated in 10 ml RPMI 1640 (Life Technologies by Life Technologies) containing 7.5% FCS (Sigma), 0.5 mg/ml collagenase type II-S (sterile filtered) (clostridiopeptidase A derived from Clostridium histolyticum; Sigma), 0.1 mg/ml trypsin inhibitor (from Glycine max; Sigma), and 0.1 mg/ml DNase I (Roche, Berlin, Germany) for 15 min at 37˚C and 5% CO 2 , with intermittent shaking.…”

Section: Preparation Of Lamina Propria Lymphocytesmentioning

confidence: 99%

Mucosal Inducible NO Synthase–Producing IgA+ Plasma Cells in Helicobacter pylori–Infected Patients

Neumann

Mueller

Moos

et al. 2016

The Journal of Immunology

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The mucosal immune system is relevant for homeostasis, immunity, and also pathological conditions in the gastrointestinal tract. Inducible NO synthase (iNOS)–dependent production of NO is one of the factors linked to both antimicrobial immunity and pathological conditions. Upregulation of iNOS has been observed in human Helicobacter pylori infection, but the cellular sources of iNOS are ill defined. Key differences in regulation of iNOS expression impair the translation from mouse models to human medicine. To characterize mucosal iNOS-producing leukocytes, biopsy specimens from H. pylori–infected patients, controls, and participants of a vaccination trial were analyzed by immunohistochemistry, along with flow cytometric analyses of lymphocytes for iNOS expression and activity. We newly identified mucosal IgA-producing plasma cells (PCs) as one major iNOS+ cell population in H. pylori–infected patients and confirmed intracellular NO production. Because we did not detect iNOS+ PCs in three distinct infectious diseases, this is not a general feature of mucosal PCs under conditions of infection. Furthermore, numbers of mucosal iNOS+ PCs were elevated in individuals who had cleared experimental H. pylori infection compared with those who had not. Thus, IgA+ PCs expressing iNOS are described for the first time, to our knowledge, in humans. iNOS+ PCs are induced in the course of human H. pylori infection, and their abundance seems to correlate with the clinical course of the infection.

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Section: Preparation Of Lamina Propria Lymphocytesmentioning

confidence: 99%

Mucosal Inducible NO Synthase–Producing IgA+ Plasma Cells in Helicobacter pylori–Infected Patients

Neumann

Mueller

Moos

et al. 2016

The Journal of Immunology

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“…Although data on mucosal HIV-specific immunity during the acute phase of infection are scant, recent studies have investigated HIV-specific T-cell immunity in rectal biopsies of chronically HIV-infected individuals. 63,64 Initially, these studies were restricted to the physical examination of HIVspecific CD8 T cells using peptide-major histocompatibility complex tetramer technology. 63 These studies demonstrated that HIV-specific CD8 T cells were detectable in the GI tract and that the frequency of HIV-specific CD8 T cells in the GI tract was similar to that observed in peripheral blood, approximately 1% of CD8 T cells.…”

Section: Gastrointestinal Hiv-specific Immune Responsesmentioning

confidence: 99%

“…63,64 Initially, these studies were restricted to the physical examination of HIVspecific CD8 T cells using peptide-major histocompatibility complex tetramer technology. 63 These studies demonstrated that HIV-specific CD8 T cells were detectable in the GI tract and that the frequency of HIV-specific CD8 T cells in the GI tract was similar to that observed in peripheral blood, approximately 1% of CD8 T cells. 40 However, this is an overestimate, since these studies did not express the frequency of memory CD8 T cells, which are HIV-specific; moreover, almost all the GI-tract CD8 T cells belong to the memory compartment, yet naive CD8 T cells circulate in peripheral blood.…”

Section: Gastrointestinal Hiv-specific Immune Responsesmentioning

confidence: 99%

HIV infection and the gastrointestinal immune system

2008

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There has recently been a resurgence of interest in the gastrointestinal pathology observed in patients infected with HIV. The gastrointestinal tract is a major site of HIV replication, which results in massive depletion of lamina propria CD4 T cells during acute infection. Highly active antiretroviral therapy leads to incomplete suppression of viral replication and substantially delayed and only partial restoration of gastrointestinal CD4 T cells. The gastrointestinal pathology associated with HIV infection comprises significant enteropathy with increased levels of inflammation and decreased levels of mucosal repair and regeneration. Assessment of gut mucosal immune system has provided novel directions for therapeutic interventions that modify the consequences of acute HIV infection.The mucosal surface of the gastrointestinal (GI) tract forms a unique anatomical and physiological niche, serving as a predominant structural and immunological barrier against the microorganisms of the outside world. In addition, the tightly apposed enterocytes that form the single cell layer of the mucosal epithelium also absorb water and nutrients during the digestive process, which is critical to host survival. Hence, loss of the integrity of the mucosal surface results in multiple deleterious sequelae.

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“…A suspension of mucosal cells from rectosigmoid biopsies was obtained after 2 successive 0.25-mg/mL collagenase type II treatments (Sigma-Aldrich), as previously described. 22 …”

Section: Tissue Collection and Processingmentioning

confidence: 99%

Correlating cellular and molecular signatures of mucosal immunity that distinguish HIV controllers from noncontrollers

Loke

Favre

Hunt³

et al. 2010

Blood

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Optimization of methods to assess human mucosal T-cell responses to HIV infection

Cited by 95 publications

References 51 publications

Mucosal Inducible NO Synthase–Producing IgA+ Plasma Cells in Helicobacter pylori–Infected Patients

Mucosal Inducible NO Synthase–Producing IgA+ Plasma Cells in Helicobacter pylori–Infected Patients

HIV infection and the gastrointestinal immune system

Correlating cellular and molecular signatures of mucosal immunity that distinguish HIV controllers from noncontrollers

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