Optimization of multiple enzymes production by fermentation using lipid-producing Bacillus sp.

Abstract

The present study identified the pectinase-producing bacterium isolated from the contaminated broth as Bacillus sp. on 16S rDNA sequence analysis. The bacterium illustrated water-like droplets on the colony grown on the Sabouraud dextrose agar plate. It also exhibited multi-enzymes activities, such as pectinase, polygalacturonase, xylanase, and cellulase by using various agro-wastes as low-cost substrates. The orange peel was observed to be the best substrate among the agro-wastes used for maximum multi-enzymes (pectinase, polygalacturonase, xylanase, and cellulase). However, the bacterium demonstrated its capability to produce different enzymes according to the different substrates/agro-wastes used. The Plackett–Burman design was used to determine the essential influencing factors, while the Box Behnken design response surface methodology was for optimizing cultural conditions. At their optimal conditions (40°C incubation temperature, 24 h of incubation period, 1% w/v orange peel, and 2% v/v inoculum volume), the bacterium exhibited the maximum pectinase (9.49 ± 1.25 U/ml) and xylanase (16.27 ± 0.52 U/ml) activities. Furthermore, the study explored the ability of the bacterium to produce bacterial lipids and observed about 25% bacterial lipid content on a dry weight basis. Therefore, the bacterium is a good candidate for producing important multi-enzymes and subsequent agro-waste degradation controlling the environment, and facilitating waste management. Also, the bacterium can be a potential feedstock in producing renewable biofuel.

Bacteriophage Challenges in Industrial Processes: A Historical Unveiling and Future Outlook

“…The discovery of penicillin caused the development of the medical industry and led scientists to desire to discover new antibiotics and drugs. At present, fermentation processes are also used in the industrial production of amino acids [ 24 ], enzymes [ 25 ], and other chemicals [ 26 ]. Many drugs (e.g., insulin [ 27 ]) are produced using genetically modified organisms, often bacteria.…”

Production Optimization, Partial Characterization, and Gluten-Digesting Ability of the Acidic Protease from Clavispora lusitaniae PC3

“…For this, a matrix of 12 experiments was used to evaluate the effect of the three dummy variables (D, H, and K) and eight reel variables (factors) studied. Each factor was studied at two levels high (+1) and low (−1) [33,34] namely, A: Temperature, B: Moisture, C: Inoculum, E: Glucose, F: «Corn steep liquor»: G: (NH 4 ) SO 4 , I: NaCl, and J :Time fermentation (Table 1).…”

“…In biotechnology, there is a growing recommendation for the use of statistical experimental designs, and several scientists achieved the optimization of protease production from microbial sources using the statistical designs [32,34,57]. In biotechnology, there is a growing recommendation for the use of statistical experimental designs, and several scientists achieved the optimization of protease production from microbial sources using the statistical designs [32,34,57].…”