Optimization of Murrayafoline A ethanol extraction process from the roots of <i>Glycosmis stenocarpa</i>, and evaluation of its Tumorigenesis inhibition activity on Hep-G2 cells

Trần, Quốc Toàn; Nguyen, Thanh Duong; Luu, Van Huyen; Nghị, Đỗ Hữu; Le, Xuan Que; Nguyen, Phi Hung; Cuong, Nguyen Manh; Luu, Van Chinh; Pham, Minh Quan; Vu, Thi Huyen; Pham, Tri Nhut; Pham, Dung Thuy Nguyen

doi:10.1515/chem-2021-0067

Cited by 3 publications

(2 citation statements)

References 19 publications

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“…The extraction yield and TSC were dependent variables, while ultrasonic power, extraction temperature, and time were independent variables. The variables were applied to the second order polynomial equation, as described by Tran et al (2021) [32]. Design-Expert 12.0.0 software (Stat-Ease, MN, USA) was used to produce 17 sets of process factors (Tables 1 and 2).…”

Section: Experiments Design and Optimizationmentioning

confidence: 99%

Optimization of Total Saponin Extraction from Polyscias fruticosa Roots Using the Ultrasonic-Assisted Method and Response Surface Methodology

Khoảng¹,

Huyen²,

Chung³

et al. 2022

Processes

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Polyscias fruticosa (L.) Harms is commonly used for medicinal purposes in Vietnam. In traditional medicine, the plant is used to cure ischemia, reduce inflammation, and increase cerebral blood circulation. Triterpene saponins are the major chemical constituents found in the roots of P. fruticosa. This compound exhibited a broad spectrum of biological effects, including lowering blood sugar, suppressing tumor growth and inflammation. This study focused on optimizing the process of total saponins extraction from P. fruticosa roots using the ultrasonic-assisted extraction (UAE) method, ethanol solvent and response surface methodology, and Box–Behnken design model, then evaluating the cytotoxic effect against some cancer cell lines. The results showed that under the optimal conditions, including an extraction temperature of 60 °C and ultrasonic power of 185 W in 65 min, the maximum extraction yield and total saponin content were 14.51 ± 1.15% and 41.24 ± 1.68 mg/g, respectively. Moreover, the saponin extract had cytotoxic effects against A549, HepG2, PC-3, and Hela. The results of this study confirmed that triterpene saponin is an important chemical component which is present in a high content in P. fruticosa roots and gives rise to significant biological activities. In addition, UAE can be used as a highly efficient method for triterpene saponins extraction from P. fruticosa roots.

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Section: Experiments Design and Optimizationmentioning

confidence: 99%

Optimization of Total Saponin Extraction from Polyscias fruticosa Roots Using the Ultrasonic-Assisted Method and Response Surface Methodology

Khoảng¹,

Huyen²,

Chung³

et al. 2022

Processes

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“…Murrayafoline A (MuA) is a plant alkaloid isolated from the root of several species of the genus Clausena (Rutaceae) and Murraya [8,9]. This compound has many biological activities such as anti-cell growth (SW480, HCT116, MoLT-4 (acute lymphoblastic leukemia), HOP-18 (lung cancer) and LS174T) and anti-inflammator [10][11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Synthesis of glycyrrhetinic acid-modified liposomes to deliver Murrayafoline A for treatment of hepatocellular carcinoma

Dinh

Phan

et al. 2022

J Mater Sci: Mater Med

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Hepatocellular carcinoma is a common type of cancer associated with a high mortality rate. Among several bioactive compounds, Murrayafoline A (MuA) has been proved as a bio substance that exhibits great potentials in treating liver cancer. In order to overcome the high cytotoxicity and low solubility of MuA, a delivery system based on nanocarriers is necessary to deliver MuA towards the desired target. In the present study, 18β-glycyrrhetinic acid (GA), which is known as a ligand for liver targeting, was used to construct the cholesterol-poly (ethylene glycol)-glycyrrhetinic acid (GA-PEG-Chol) conjugate and liposome for MuA administration. The compound was then examined for therapeutic efficacy and safety in HUVEC and HepG2 cells in 2D and 3D cell cultures. Results have shown that MuA-loaded liposomes had IC50 value of 2 µM in HepG2 and had the cytosolic absorption of 8.83 ± 0.97 ng/105 cells, while The IC50 value of MuA-loaded liposomes in HUVEC cell lines was 15 µM and the the cytosolic absorption was recorded as 3.62 ± 0.61 cells. The drug test on the 3D cancer sphere platform of the HepG2 cancer sphere showed that MuA-loaded GA liposomes had the highest efficacy at a concentration of 100 µg/mL. In short, these results suggest that MuA-loaded GA liposomes have the potential for maintenance drug delivery and liver targeting.

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Synthesis and Cytotoxic Activities of Novel Ether Conjugates of Dihydroartemisinin and Zerumbone: Evidenced by Integrating Network Pharmacology and In Vitro Assay

Hung Truong,

Hung Nguyen,

Nghi Do

et al. 2024

Chemistry & Biodiversity

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O‐alkylation of the hydroxy compounds, including acetaminophen, starting compounds for the synthesis of the drug, and natural compounds with the bromides of dihydroartemisinin (DHA) and zerumbone, produced twenty novel ether conjugates 15a‐j and 16a‐j, respectively. Their structures were elucidated by 1D‐, 2D‐NMR, and HRMS data. Their in vitro cytotoxic activity was screened using three cancer cell lines: HepG2, HeLa, and PC‐12. The results showed that eight out of ten conjugates in series 15a‐j containing DHA skeleton exhibited activity against the tested cell lines, with IC50 values ranging from 4.26‐47.37 µM. Notably, all conjugates in series 16a‐j containing zerumbone scaffolds inhibited the growth of HepG2, HeLa, and PC12 with IC50 in the range of 4.46‐35.07 µM. Using network pharmacology and molecular docking to target anti‐liver cancer in the above 20 synthetic compounds, 271 intersection targets were discovered, including 5 targets with high degree values (EGFR, ESR1, AKT1, MDM2, and NFKB1). Artemisinin derivative 15i gave the highest binding energy for targets AKT1, EGFR, and NFKB1, while zerumbone‐murrayafoline A ether 16g in the remaining series also gave the highest energy for proteins EGFR, AKT1, and NFKB1

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Optimization of Murrayafoline A ethanol extraction process from the roots of Glycosmis stenocarpa, and evaluation of its Tumorigenesis inhibition activity on Hep-G2 cells

Cited by 3 publications

References 19 publications

Optimization of Total Saponin Extraction from Polyscias fruticosa Roots Using the Ultrasonic-Assisted Method and Response Surface Methodology

Optimization of Total Saponin Extraction from Polyscias fruticosa Roots Using the Ultrasonic-Assisted Method and Response Surface Methodology

Synthesis of glycyrrhetinic acid-modified liposomes to deliver Murrayafoline A for treatment of hepatocellular carcinoma

Synthesis and Cytotoxic Activities of Novel Ether Conjugates of Dihydroartemisinin and Zerumbone: Evidenced by Integrating Network Pharmacology and In Vitro Assay

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