Optimization of neutral protease production from Bacillus subtilis: Using agroindustrial residues as substrates and response surface methodology

Abstract

Statistically based experimental designs were applied to optimize the fermentation medium and cultural conditions for the maximization of neutral protease using three agroindustrial residues (cassava pulp, soybean meal, and wheat bran) and Bacillus subtilis DES-59. The Plackett-Burman design was used to evaluate the effects of variables such as the concentration of substrates, initial pH, shaker's rotating speed, temperature, inoculum size, and incubation time. Among the eight parameters, three significant variables (cassava pulp, soybean meal, and inoculum size) were selected for the optimization study, in which a central composite design was used to optimize the concentrations of cassava pulp and soybean meal and inoculum size and investigate the interactive effects of the three variables. The optimal parameters obtained from response surface methodology are 37.78 g/L of cassava pulp, 15 g/L of soybean meal, and 6.5% (v/v) of inoculum size, respectively, resulting in a maximum neutral protease activity of 4107 ± 122 U/mL.

Protease Produced by Endophytic Fungi: A Systematic Review

“…Nowadays, the analysis of variables through statistical methodologies are widely used to optimize the enzymatic production of several microorganisms. They are quick and easy methodologies and are quite reliable [40]. However, few studies using statistical techniques have been conducted for protease optimization by endophytic fungi.…”

Ciências biológicas: as diversas manifestações da vida

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