Optimization of non-detergent treatment for enveloped virus inactivation using the Taguchi design of experimental methodology (DOE)

Khosravi, Roya; Hosseini, Seyed Nezamedin; Javidanbardan, Amin; Khatami, Maryam; Kaghazian, Hooman; Nasab, Seyed Dawood Mousavi

doi:10.1080/10826068.2019.1599398

Cited by 1 publication

(1 citation statement)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The refrigerated storage conditions for retinal pigment epithelial cells [ 118 ] and epithelial cell sheets [ 119 ] were optimized through full and fractional factorial screening. In addition, the virus inactivation process of Vero cells [ 120 ] was investigated using an orthogonal array. Optimal freezing conditions for CHO and human embryonic kidney (HEK) cells were identified by RSM through the modelling of the first doubling time after the cell thawing [ 121 ].…”

Section: Other Cell Expansion Cell Differentiation and Cell-materials Development Processesmentioning

confidence: 99%

Clever Experimental Designs: Shortcuts for Better iPSC Differentiation

Yasui

Sekine

Taniguchi

2021

Cells

View full text Add to dashboard Cite

For practical use of pluripotent stem cells (PSCs) for disease modelling, drug screening, and regenerative medicine, the cell differentiation process needs to be properly refined to generate end products with consistent and high quality. To construct and optimize a robust cell-induction process, a myriad of cell culture conditions should be considered. In contrast to inefficient brute-force screening, statistical design of experiments (DOE) approaches, such as factorial design, orthogonal array design, response surface methodology (RSM), definitive screening design (DSD), and mixture design, enable efficient and strategic screening of conditions in smaller experimental runs through multifactorial screening and/or quantitative modeling. Although DOE has become routinely utilized in the bioengineering and pharmaceutical fields, the imminent need of more detailed cell-lineage specification, complex organoid construction, and a stable supply of qualified cell-derived material requires expedition of DOE utilization in stem cell bioprocessing. This review summarizes DOE-based cell culture optimizations of PSCs, mesenchymal stem cells (MSCs), hematopoietic stem cells (HSCs), and Chinese hamster ovary (CHO) cells, which guide effective research and development of PSC-derived materials for academic and industrial applications.

show abstract