2024
DOI: 10.1063/5.0192315
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Optimization of OpenCV based spot identification method for surface plasmon resonance imaging

Zhiyou Wang,
Feiyu Liu,
Wenxuan Xiao
et al.

Abstract: In this work, we focus on the OpenCV based microarray recognition method for Surface Plasmon Resonance Imaging (SPRi), proposing the hit-ratio of global light pixels and coverage of the potential spots in a microarray as the criteria for identification evaluation in SPRi data. We optimized the design of the ellipse fitting strategy by analyzing the impact of different parameters in the method. After optimization of the parameters, the accuracy of microarray recognition was successfully increased to over 90%. T… Show more

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