Optimization of parameters for obtaining callus, suspension, and root cultures of meadowsweet (filipendula ulmaria) to isolate the largest number of biologically active substances with geroprotective properties

Dyshlyuk, Lyubov; Vesnina, Anna; Dmitrieva, Anastasia; Kozlova, Oksana; Prosekov, Alexander

doi:10.1590/1519-6984.257074

Cited by 4 publications

(2 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Geroprotectors are compounds that increase life expectancy and improve its quality. Animal tests revealed more than two hundred geroprotectors [ 7 , 8 ]. Geriatrics are intended to treat senior citizens or improve their quality of life, while antioxidants are meant for the young and mature populations [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Ex Vivo and In Vitro Antiaging and Antioxidant Extract Activity of the Amelanchier ovalis from Siberia

Asyakina

Atuchin

Drozdova

et al. 2022

IJMS

Self Cite

View full text Add to dashboard Cite

Phenolic acids are biologically active substances that prevent aging and age-related diseases, e.g., cancer, cardiovascular diseases, Alzheimer’s disease, Parkinson’s disease, etc. Cellular senescence is related to oxidative stress. The Siberian Federal District is rich in medicinal plants whose extracts contain phenolic acids. These plants can serve as raw materials for antiaging, antioxidant food supplements, and Amelanchier ovalis is one of them. In the present research, we tested the phytochemical profile of its extract for phenolic acids. Its geroprotective and antioxidant properties were studied both ex vivo and in vitro using Saccharomyces cerevisiae Y-564 as a model organism. The chromotographic analysis revealed gallic, p-hydroxybenzoic, and protocatechuic acids, as well as derivatives of chlorogenic and gallic acids. The research involved 0.25, 0.5, and 1.0 mg/mL extracts of Amelanchier ovalis, all of which increased the growth and lifespan of yeast cells. In addition, the extracts increased the survival rate of yeast under oxidative stress. An in vitro experiment also demonstrated the antioxidant potential of Amelanchier ovalis against ABTS radicals. Therefore, the Amelanchier ovalis berry extract proved to be an excellent source of phenolic acids and may be recommended as a raw material for use in antioxidant and geroprotective food supplements.

show abstract

Section: Introductionmentioning

confidence: 99%

Ex Vivo and In Vitro Antiaging and Antioxidant Extract Activity of the Amelanchier ovalis from Siberia

Asyakina

Atuchin

Drozdova

et al. 2022

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…Adhering to a proper diet (consumption of fruits, vegetables, and various antioxidant-rich, anti-inflammatory, and other bioactive substances (BAS)) contributes to improving human health and healthy aging. However, due to economic, climatic, geographical, and other limitations, the systematic consumption of certain products is difficult [1, 2]. Therefore, the development of biologically active supplements (BAS) is relevant.…”

Section: Introductionmentioning

confidence: 99%

Study of the hepatotoxicity of trans-coric acid isolated fromScutellaria baicalensisinin vivoexperiments

Mikhailovna,

Dmitrievna,

Sergeevna

et al. 2024

Preprint

Self Cite

View full text Add to dashboard Cite

Biologically active supplements (BAS) based on plant material, possessing a wide range of beneficial properties (antioxidant, anti-inflammatory, antimicrobial, etc.), are becoming increasingly popular worldwide. Such supplements are readily available and can be used without consulting a healthcare professional. Consequently, the extent of their use is underestimated by doctors. Plant hepatotoxicity is not uncommon, but the frequency and manifestation are not sufficiently characterized. This study is dedicated to investigating the hepatotoxicity of trans-coric acid isolated from the root culture of Scutellaria baicalensis in in vivo experiments. This work aims to evaluate the hepatotoxicity of trans-coric acid on male rats at doses of 50.0 and 100.0 mg/kg. During the experiment, it was found that trans-coric acid, isolated from the root culture extract of Scutellaria baicalensis, does not induce hepatotoxicity in the presented doses in model animals with 14-day intragastric administration. Thus, this study demonstrates that trans-coric acid can be safely used as a component of BAS for the prevention of chronic diseases, thereby promoting healthy aging

show abstract

The effect of biologically active substances of thyme (Thymus vulgaris L.) on the growth of yeast cells

Fedorova,

Drozdova,

Milentyeva

et al. 2022

Vestn. Voronež. gos. univ. inž. tehnol.

View full text Add to dashboard Cite

Prolongation of life expectancy and improvement of phenotypes directly related to the aging process have been the objects of many studies. Some of the known methods of increasing life expectancy, including dietary restrictions and genetic manipulation, are difficult to apply to all people, and their side effects are difficult to predict. It is for this reason that it becomes important to find those biologically active compounds that can act as anti-aging agents or can induce prolongation of life due to various metabolic processes inside the cell. The medicinal plant SFO thyme (Thymus vulgaris L.) is a source of many active compounds that can affect life expectancy. The present study was conducted to evaluate the effect of biologically active compounds of the extract of the callus culture of Thymus vulgaris L., dried by spray drying at different temperature conditions (60 ℃, 90 ℃, 120 ℃), on the growth of the experimental model Saccharomyces cerevisiae Y-564. Anti-aging effect of dry extract of callus culture Thymus vulgaris L. with concentrations of 0.25 mg/ml, 0.50 mg/ml and 1.00 mg/ml on a model yeast organism, the yeast cells were evaluated by increasing the biomass of yeast cells. The results of the studies showed that the best variant of the effect of the growth properties of the biomass of the yeast suspension S. cerevisiae Y-564 showed an extract of the callus culture Thymus vulgaris L., dried at a temperature of 90 ℃, with a concentration of 0.50 mg / ml. It was under such conditions that a stimulating increase in the biomass of the test culture was observed. These results of this study provide new insights into the mechanisms by which biologically active compounds extracted from the callus culture of Thymus vulgaris L. can slow down the aging process.

show abstract

Optimization of parameters for obtaining callus, suspension, and root cultures of meadowsweet (filipendula ulmaria) to isolate the largest number of biologically active substances with geroprotective properties

Cited by 4 publications

References 37 publications

Ex Vivo and In Vitro Antiaging and Antioxidant Extract Activity of the Amelanchier ovalis from Siberia

Ex Vivo and In Vitro Antiaging and Antioxidant Extract Activity of the Amelanchier ovalis from Siberia

Study of the hepatotoxicity of trans-coric acid isolated fromScutellaria baicalensisinin vivoexperiments

The effect of biologically active substances of thyme (Thymus vulgaris L.) on the growth of yeast cells

Contact Info

Product

Resources

About