Optimization of parameters for preparation of docetaxel-loaded PLGA nanoparticles by nanoprecipitation method

Shi, Wei; Zhang, Zhan-jie; Yin, Yuan; Xing, Enming; Qin, You; Peng, Zenghui; Zhang, Zhiping; Yang, Kunyu

doi:10.1007/s11596-013-1192-x

Cited by 15 publications

(18 citation statements)

References 29 publications

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“…SE-NPs exhibited higher entrapment efficiency than NR-NPs; this just corresponds to the results confirmed by several researchers [41,42]. We thought that this may be due to the larger size of SE-NP than NR-NP which could support a larger space for Sal encapsulation.…”

Section: Resultssupporting

confidence: 89%

Comparative studies of salinomycin-loaded nanoparticles prepared by nanoprecipitation and single emulsion method

Wang

Ren

et al. 2014

Nanoscale Res Lett

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To establish a satisfactory delivery system for the delivery of salinomycin (Sal), a novel, selective cancer stem cell inhibitor with prominent toxicity, gelatinase-responsive core-shell nanoparticles (NPs), were prepared by nanoprecipitation method (NR-NPs) and single emulsion method (SE-NPs). The gelatinase-responsive copolymer was prepared by carboxylation and double amination method. We studied the stability of NPs prepared by nanoprecipitation method with different proportions of F68 in aqueous phase to determine the best proportion used in our study. Then, the NPs were prepared by nanoprecipitation method with the best proportion of F68 and single emulsion method, and their physiochemical traits including morphology, particle size, zeta potential, drug loading content, stability, and in vitro release profiles were studied. The SE-NPs showed significant differences in particle size, drug loading content, stability, and in vitro release profiles compared to NR-NPs. The SE-NPs presented higher drug entrapment efficiency and superior stability than the NR-NPs. The drug release rate of SE-NPs was more sustainable than that of the NR-NPs, and in vivo experiment indicated that NPs could prominently reduce the toxicity of Sal. Our study demonstrates that the SE-NPs could be a satisfactory method for the preparation of gelatinase-responsive NPs for intelligent delivery of Sal.

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Section: Resultssupporting

confidence: 89%

Comparative studies of salinomycin-loaded nanoparticles prepared by nanoprecipitation and single emulsion method

Wang

Ren

et al. 2014

Nanoscale Res Lett

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“…It should be emphasized that determining the amount of loaded drug inside polymeric matrix is a crucial step in characterization of nanoparticle. To date, docetaxel has been quantitatively assayed by means of different HPLC [15]- [17] [41] or UV [18] set-ups reporting different assay outcomes. for instance, Esmaeili et al [12] reported their method LOD to be 50 ng/ml with a retention time around 12 min.…”

Section: Nanoparticle Drug Loading and Encapsulation Efficiencymentioning

confidence: 99%

Application of a Rapid ESI-MS/MS Method for Quantitative Analysis of Docetaxel in Polymeric Matrices of PLGA and PLGA-PEG Nanoparticles through Direct Injection to Mass Spectrometer

Rafiei¹,

Michel²,

Haddadi

2015

AJAC

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Docetaxel is a member of taxan family of antineoplastic agents widely used in cancer chemotherapy. However, application of conventional chemotherapy with commercial formulation has been accompanied with matters of concern regarding drug's biodistribution, pharmacokinetics, and pharmacodynamics. Polymeric nanoparticles have been widely used as unique drug delivery vehicles to circumvent such problems. Docetaxel-loaded poly (lactide-co-glycolide) (PLGA) and poly (lactide-co-glycolide)-poly (ethylene glycol) (PLGA-PEG) nanoparticles fit well in modifying drug's pharmacokinetic characteristics as intravenous (IV) sustained-release delivery vehicles. In such circumstances, characterization of nanoparticles in terms of their drug-payload would be a necessary step. The majority of studies have used HPLC analysis method for docetaxel quantitation in polymeric nanoparticles. Herein, a rapid ESI-MS/MS method for quantitative analysis of docetaxel in polymeric matrices of PLGA and PLGA-PEG nanoparticles through direct injection to mass spectrometer has been developed and validated. The assay was validated over a range of 3.9 -1000 ng/ml and 125 -16,000 ng/ml. Samples were directly injected to the instrument through an isocratic elution (0.1% formic acid in methanol) and detection was performed on a Hybrid Triple Quadrupole/Linear Ion trap mass spectrometer with multiple reaction monitoring (MRM) mode via positive electrospray ionization (ESI) source. The run time and retention time were 2 and 0.6 minutes respectively. The method demonstrated acceptable level of accuracy and precision and was successfully applied for quantitative analysis of docetaxel in polymeric nanoparticles of PLGA and PLGA-PEG.

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“…Actually, AuNPs seem to be the most promising in this field. Shi et al [164] reported that deposition of AuNPs into fibrous decellularized matrices improved resulting morphology of cardiac cells grown within these scaffolds. This provides better striation behavior and improved electrical coupling of proteins.…”

Section: Electrical Propertiesmentioning

confidence: 99%

Nanostructured Materials for Artificial Tissue Replacements

Pryjmaková

Kaimlová

Hubáček

et al. 2020

IJMS

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This paper review current trends in applications of nanomaterials in tissue engineering. Nanomaterials applicable in this area can be divided into two groups: organic and inorganic. Organic nanomaterials are especially used for the preparation of highly porous scaffolds for cell cultivation and are represented by polymeric nanofibers. Inorganic nanomaterials are implemented as they stand or dispersed in matrices promoting their functional properties while preserving high level of biocompatibility. They are used in various forms (e.g., nano- particles, -tubes and -fibers)—and when forming the composites with organic matrices—are able to enhance many resulting properties (biologic, mechanical, electrical and/or antibacterial). For this reason, this contribution points especially to such type of composite nanomaterials. Basic information on classification, properties and application potential of single nanostructures, as well as complex scaffolds suitable for 3D tissues reconstruction is provided. Examples of practical usage of these structures are demonstrated on cartilage, bone, neural, cardiac and skin tissue regeneration and replacements. Nanomaterials open up new ways of treatments in almost all areas of current tissue regeneration, especially in tissue support or cell proliferation and growth. They significantly promote tissue rebuilding by direct replacement of damaged tissues.

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Optimization of parameters for preparation of docetaxel-loaded PLGA nanoparticles by nanoprecipitation method

Cited by 15 publications

References 29 publications

Comparative studies of salinomycin-loaded nanoparticles prepared by nanoprecipitation and single emulsion method

Comparative studies of salinomycin-loaded nanoparticles prepared by nanoprecipitation and single emulsion method

Application of a Rapid ESI-MS/MS Method for Quantitative Analysis of Docetaxel in Polymeric Matrices of PLGA and PLGA-PEG Nanoparticles through Direct Injection to Mass Spectrometer

Nanostructured Materials for Artificial Tissue Replacements

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