Optimization of Plant Growth Regulators for In Vitro Mass Propagation of a Disease-Free ‘Shine Muscat’ Grapevine Cultivar

Abstract

This study addresses the propagation challenges faced by ‘Shine Muscat’, a newly introduced premium grapevine cultivar in South Korea, where multiple viral infections pose considerable economic loss. The primary objective was to establish a robust in vitro propagation method for producing disease-free grapes and to identify effective plant growth regulators to facilitate large-scale mass cultivation. After experimentation, 2.0 µM 6-benzyladenine (BA) exhibited superior shoot formation in the Murashige and Skoog medium compared with kinetin and thidiazuron. Conversely, α-naphthaleneacetic acid (NAA) hindered shoot growth and induced callus formation, while indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA) demonstrated favorable root formation, with IBA showing better results overall. Furthermore, inter simple sequence repeat analysis confirmed the genetic stability of in vitro-cultivated seedlings using 2.0 μM BA and 1.0 μM IBA, validating the suitability of the developed propagation method for generating disease-free ‘Shine Muscat’ grapes. These findings offer promising prospects for commercial grape cultivation, ensuring a consistent supply of healthy grapes in the market.

In Vitro Collection for the Safe Storage of Grapevine Hybrids and Identification of the Presence of Plasmopara viticola Resistance Genes

“…Plant tissue culture media have a profound effect on both the induction of adventitious shoot formation and the promotion of axillary shoot proliferation [ 53 , 62 ]. MS medium [ 63 ] is often used with success to grow grapevines in in vitro culture [ 64 , 65 , 66 ], and its exogenous supplementation with phytohormones serves as a tool to regulate various processes in plant tissue cultures. Auxins and cytokinins are the most commonly used phytohormones and are often used in combination [ 53 , 67 , 68 ].…”

In Vitro Microrhizome Production, Genetic Homogeneity Assessment, and Field Performance Evaluation in Ginger

“…Therefore, it is imperative to cultivate pathogen-free seedling rhizomes via a cost-effective method with a high multiplication rate to ensure success in cultivating this crop species. In vitro culture has been extensively used to produce disease-free plants in numerous crop species [6,7], including ginger [8,9]. However, ginger plants regenerated in vitro generally produce low yields and small rhizomes that are not suitable for commercial use in the first growing season [10].…”