Optimization of polyphenolic compounds extraction methods from Okra stem

Amirabbasi, Somayeh; Elhamirad, Amir Hossein; Saeediasl, Mohammad Reza; Armin, Mohammad Ali; Ziaolhagh, Seyed Hamid Reza

doi:10.1007/s11694-020-00641-8

Cited by 15 publications

(10 citation statements)

References 52 publications

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“…Previous studies established that solvent type affects the amount and rate of total polyphenols extracted from plant materials due to properties such as polarity differences [36]. Unlike the present study that used water extraction solvent, earlier studies extracted total polyphenols from A. esculentus using methanol, ethanol, and their varied combination with water [21,22]. Although such solvents resulted in a good yield of total polyphenols [37], the use of organic solvent could not provide a more practical application to the traditional use of A. esculentus, thus, rendering previous study findings; of limited use at the community level.…”

Section: Discussionmentioning

confidence: 81%

“…Unlike the present study that used water extraction solvent, earlier studies extracted total polyphenols from A . esculentus using methanol, ethanol, and their varied combination with water [ 21 , 22 ]. Although such solvents resulted in a good yield of total polyphenols [ 37 ], the use of organic solvent could not provide a more practical application to the traditional use of A .…”

Section: Discussionmentioning

confidence: 99%

“…Extraction methods, extraction time, temperature, and type of solvents highly influence polyphenols' extraction from plants, including A. esculentus. Previous studies have used water, methanol, ethanol, acetone, ethyl acetate, and their mixtures to extract polyphenols from flowers, fruits, leaves, seeds, and stem of A. esculentus [21,22]. Although these simple alcohols (methanol, ethanol), acetone, and ethyl acetate are environmentally friendly organic solvents [23], they are not adequately reflecting the traditional method of preparations which essentially involves using hot water maceration [6,7].…”

Section: Introductionmentioning

confidence: 99%

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Total polyphenols and antihyperglycemic activity of aqueous fruits extract of Abelmoschus esculentus: Modeling and optimization of extraction conditions

et al. 2021

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Aqueous fruits extract of Abelmoschus esculentus (L.) Moench (Malvaceae) has been used traditionally in several communities to alleviate elevated blood glucose levels. However, optimized extraction conditions have not been reported. Thus, this study determined the optimal extraction conditions for extracting polyphenols from A. esculentus fruits and evaluated antihyperglycemic activity in vivo. Extraction time, temperature, and solid-to-solvent ratio were optimized using Response Surface Methodology (RSM). Total polyphenols and flavonoids were quantified using the Folin-Ciocalteu and aluminium chloride colorimetric methods, respectively. The fingerprint and quantification of quercetin—a major flavonoid with an antihyperglycemic effect was done using the chromatographic method. The antihyperglycemic activity was determined in a high-fat diet-Streptozotocin rat model. The rats were assigned to five groups (n = 6): Group 1 and 2 were normal and diabetic control received distilled water 1 mL/100g; Treatment group 3 and 4 received standardized A. esculentus fruit extract (AEFE) at a dose of 100 and 200 mg/kg, respectively; Group 5 received 5 mg/kg glibenclamide. All treatments were given orally for 14 days. Measurements of fasting plasma glucose (FPG) and body weight were done weekly. The RSM quadratic model predicted total polyphenols of 22.16 mg GAE/g DW. At optimal conditions of a solid-to-solvent ratio of 5%, extraction time 1 h, and extraction temperature of 70°C, confirmation experiments yield 20.2 [95% CI; 16.7 to 27.6] mg GAE/g DW, implying the model successfully predicted total polyphenols. The extract HPLC fingerprint showed 13 characteristic peaks with 0.45 ± 0.02 μg/g DW of quercetin. Compared with diabetic control, the standardized AEFE reduced FPG level dose-dependently (P < 0.001) with an EC50 of 141.4 mg/kg. Together, at optimal extraction conditions, extract with a high content of total polyphenols and good antihyperglycemic activity can be obtained. Studies are needed to identify additional polyphenolic compounds and determine their antidiabetic effects.

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Section: Discussionmentioning

confidence: 81%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Total polyphenols and antihyperglycemic activity of aqueous fruits extract of Abelmoschus esculentus: Modeling and optimization of extraction conditions

et al. 2021

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“…Also the extraction time is significantly reduced by these new techniques ( 4 , 5 ). Meanwhile each extraction technique has its own advantages and limitations; MAE has demonstrated its high efficiency for a fast extraction of good quality bioactive compounds from natural sources when the solvent is well chosen ( 6 ). Okra seeds have been reported as the richest part of the fruits in polyphenols and flavonoids in general, and also in demonstrated antidiabetic compounds such as quercetin and its derivatives in particular ( 3 ).…”

Section: Introductionmentioning

confidence: 99%

“…So, determination of experimental conditions for maximum extraction of polyphenolic compounds from okra seeds using a more efficient technique is quite urgent in order to reduce pollution, solvent and time wastage and above all improve the extraction yield, the quality and the bioactivity of the extracts ( 8 – 10 ). Geng et al ( 11 ) have determined the optimal conditions for extracting polyphenols from okra flowers using conventional maceration technique while Amirabbasi et al ( 6 ) established the conditions for a maximal extraction of polyphenols from okra stems using MAE and UAE. This study aimed to determine the optimal conditions for extraction of polyphenols from okra seeds by a face centered composite design, using MAE and to evaluate the antidiabetic activity of the extract obtained.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant and antidiabetic activities of a polyphenol rich extract obtained from Abelmoschus esculentus (okra) seeds using optimized conditions in microwave-assisted extraction (MAE)

Woumbo

Kuate²,

Tamo³

et al. 2022

Front. Nutr.

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Functional foods have gained popularity in recent decades. They are exploited for their bioactive compounds like polyphenols, which are highly demanded in cosmetic, pharmaceutical and nutraceutical industries. However, extractive techniques and conditions used up to recently are almost obsolete and must be optimized for higher efficiency. The current study aimed to evaluate the antidiabetic potential of an optimized extract of Abelmoschus esculentus (okra) seeds. The optimal conditions for extracting polyphenolic compounds from okra seeds were determined using Microwave Assisted Extraction (MAE). A Face Center Composite Design (FCCD) was used for optimization. Solvent/dry matter ratio, wavelength and time were considered while the response studied was the polyphenolic content. The extract obtained at optimal conditions was characterized using Thin Layer Chromatography (TLC) and Fourier Transform Infra-Red (FTIR) spectroscopy, then tested for its antioxidant, alpha amylase inhibitory and antidiabetic activities. Response Surface Methodology (RSM) permitted the determination of the optimal conditions for phenols extraction as: microwave power 330 W, with a solvent ratio of 97.04/1 mL/g for 9.5 min of extraction time. The optimized extract showed a phenolic content up to 86.37 ± 1.13 mg GAE/g containing quercetin and catechin as revealed by the TLC. Functional groups characteristic of polyphenols were identified on FTIR spectra, and the extract exhibited good in vitro antioxidant capacities with DPPH (2, 2-diphenyl-1-picrylhydrazyl) radical scavenging capacity and FRAP (Ferric Reducing Antioxidant Power Assay). An IC50 of 3.99 ± 0.15 μg/mL was obtained with the DPPH scavenging test. Alpha amylase inhibitory assay revealed that the optimized okra extract behaved as a non-competitive inhibitor of porcine pancreatic amylase with an IC50 of 484.17 ± 2.33 μg/mL. Antidiabetic activity of the extract was observed in streptozotocin-induced diabetic males Wistar rats, as shown by the fasting blood glucose levels, food intake, changes in body weight and serum lipid profile among others.

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Optimization of ultrasonic-assisted extraction and biological activities of total polyphenols from Toona sinensis using response surface methodology

Wen

Zeng

Mai

2022

Biomass Conv. Bioref.

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Optimization of polyphenolic compounds extraction methods from Okra stem

Cited by 15 publications

References 52 publications

Total polyphenols and antihyperglycemic activity of aqueous fruits extract of Abelmoschus esculentus: Modeling and optimization of extraction conditions

Total polyphenols and antihyperglycemic activity of aqueous fruits extract of Abelmoschus esculentus: Modeling and optimization of extraction conditions

Antioxidant and antidiabetic activities of a polyphenol rich extract obtained from Abelmoschus esculentus (okra) seeds using optimized conditions in microwave-assisted extraction (MAE)

Optimization of ultrasonic-assisted extraction and biological activities of total polyphenols from Toona sinensis using response surface methodology

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