Optimization of polysaccharides from Lycium ruthenicum fruit using RSM and its anti-oxidant activity

Liu, Zenggen; Dang, Jun; Wang, Qilan; Yu, Minfeng; Jiang, Lei; Mei, Lijuan; Shao, Yuchuan

doi:10.1016/j.ijbiomac.2013.06.042

Cited by 112 publications

(57 citation statements)

References 41 publications

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“…These structural groups show gel‐forming and thickening ability by their interacting with water molecules. The absorption peaks at 1,736, 1,650, and 1,639 cm −1 show the being of the carboxylate ion (COO) stretching band and ester carbonyl groups (CO), suggesting the presence of high quantities of uronic acids in CMP structure (Liu et al, ). The absorption peak at 1,251 cm −1 is derived from the asymmetric stretching band of glycosidic (COC) (Chang, Hsu, & Chen, ; Sun, Zhang, Zhang, & Niu, ).…”

Section: Resultsmentioning

confidence: 99%

Optimization of ultrasound‐assisted extraction of functional polysaccharides from common mullein (Verbascum thapsus L.) flowers

Babamoradi

Yousefi

Ziarati

2018

J Food Process Engineering

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Achieving a maximum extraction yield for common mullein polysaccharides (CMPs) with desirable antioxidant capacity was examined by the optimizing solvent/plant material ratio (S/PM, 10–50 v/w), and ultrasound‐assisted extraction (UAE) temperature (40–80 °C), time (15–75 min), and power (100–500 W) using response surface methodology. The highest extraction yield of polysaccharide (5.75%) from Verbascum thapsus flowers with a remarkable DPPH‐radical scavenging activity (67.66%) was resulted in the ultrasonic time of 60 min, ultrasonic temperature of 67.52 °C, ultrasonic power of 371.03 W, and S/PM of 40 v/w. The antioxidant activity and extraction yield obtained under the optimal UAE were noticeably more than those of the classical process. The cell morphology analysis by scanning electron microscopy revealed a more intact and amorphous matrix for intracellular CMPs extracted using the traditional method than UAE one. Structure of the mucilaginous materials with a minimum turbidity value was elucidated by Fourier transform‐infrared spectroscopy (FT‐IR). Practical applications Mucilages and polysaccharides have a key role in improving the emulsifying, texturizing, and antioxidant functions in food products. Verbascum thapsus is a unique herbal species with excellent medical and biological potentials. Optimization of extraction process of functional mucilages from V. thapsus flowers using powerful modeling and mathematical tools like response surface methodology (RSM) can significantly decrease the research time and cost with better results. The optimized method of ultrasound‐assisted extraction ultrasound‐assisted extraction (UAE) using RSM compared with the classical extraction methods can be a practical solution to improve quality and quantity of bioactive polysaccharides to incorporate into innovative food formulations.

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Section: Resultsmentioning

confidence: 99%

Optimization of ultrasound‐assisted extraction of functional polysaccharides from common mullein (Verbascum thapsus L.) flowers

Babamoradi

Yousefi

Ziarati

2018

J Food Process Engineering

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“…Modern pharmacological research has indicated that LRM possesses a variety of biological activities such as antioxidant (Liu et al., ; Wu, Lv, Wang, & Wang, ), immune‐enhancing (Peng, Xu, Yin, Huang, & Du, ), anti‐inflammatory (Peng, Liu, Shi, & Li, ), and anti‐fatigue (Ni et al., ). Among the chemical constituents of LRM, anthocyanins and polysaccharides have been well proved to be the major bioactive compounds (Liu et al., ; Lv, Wang, Cheng, Huang, & Wang, ; Ni et al., ; Peng et al., , ; Wu et al., ; Zheng et al., ). Nowadays, LRM is widely planted in Qinghai, Xinjiang, and Gansu region and are usually used for fresh or dried consumption, juice, jams, and wine production, and also for developing anthocyanin or polysaccharide extracts to be used as medicinal ingredients and dietary supplements.…”

Section: Introductionmentioning

confidence: 99%

Fatty Acid and Phytosterol Composition, and Biological Activities of Lycium ruthenicum Murr. Seed Oil

Zhao

Dong

et al. 2018

Journal of Food Science

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Lycium ruthenicum Murr. (LRM, Solanaceae), also called "black wolfberry" in China, is extremely tolerant drought. Although black wolfberry has seeds that are rich in oil, being considered as a potential alternative for edible oil production, little information has been found regarding its characterization. The present study evaluated the fatty acid profile of the seed oil and its phytosterol contents. Moreover, its antioxidant potential and its inhibition activities on pancreatic lipase, and cholesterol esterase (CEase) were also determined. The key fatty acids of studied oil were linoleic (74.58%), oleic (11.82%), γ-linolenic (6.60%), palmitic (4.88%), and stearic (1.40%) acids, which together comprised 99.28% of the total fatty acids. Phytosterols including 24-methylenecholesterol, campesterol, stigmasterol, dihydrolanosterol, 24-methyldesmosterol, β-sitosterol, Δ -avenasterol, cycloartenol, and Δ avenasterol were identified for the 1st time. The inhibition values (IC ) for the DPPH and ABTS radical scavenging capacities of the oil were 7.86 and 15.49 mg/mL, respectively. Additionally, LRM seed oil was also demonstrated to possess strong pancreatic lipase, and CEase inhibition activities with IC values of 12.38 and 2.63 mg/mL, respectively. The mechanism study indicated that the inhibitory manner of the oil on CEase belonged to uncompetitive inhibition of reversible inhibition. The results derived from above studies suggest that LRM seed oil could be a potential source of high value phytochemicals for developing novel functional food.

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“…The scavenging activity of TFP was further assessed with the dark purple DPPH with a maximum absorbance at 517 nm [17]. As shown in Figure 4(c), a similar trend was found for crude TFP, TFPI, and Vc as positive control, and the scavenging effects were increased in a concentration-dependent manner.…”

Section: Resultsmentioning

confidence: 60%

“…The F -value ( F = 35.72) and p value ( p < 0.01) showed that the model was significant. The significance of the model was also determined by the test of lack of fit, while the F -value of 5.55 and the associated p value of 0.0566 were insignificant, respectively, indicating that the model was accurate enough to predict the relevant response [9, 17]. …”

Section: Resultsmentioning

confidence: 99%

“…The goodness-of-fit of the model was also evaluated by the determination coefficient ( R 2 = 0.9728) and adjusted determination coefficient (Adj- R 2 = 0.9455), which indicated that 97.28% of the variations could be illustrated by the fitted model and 94.55% of the total variations were explained by the model [17]. Furthermore, Adj- R 2 was slightly smaller than R 2 and the predicted determination coefficient (Pred- R 2 = 0.8508) was also found to be smaller and very close to Adj- R 2 .…”

Section: Resultsmentioning

confidence: 99%

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Polysaccharides fromTrichosanthes Fructusvia Ultrasound-Assisted Enzymatic Extraction Using Response Surface Methodology

Chen

Shen

et al. 2017

BioMed Research International

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An efficient procedure for ultrasound-assisted enzymatic extraction of crude polysaccharides from Trichosanthes Fructus (crude TFP) using response surface methodology (RSM) was developed. The Box–Behnken design was applied to optimize the effects of pH (X1), enzyme amount (X2), extraction temperature (X3), and liquid-to-solid ratio (X4) on the extraction. The statistical analysis indicated that the independent variables (X4, X2, and X3), the quadratic coefficients (X12, X22, X32, and X42), and the interaction coefficient (X1X3) had significant impact on the yield of crude TFP. The optimal conditions were determined as follows: pH 4.5, enzyme amount 5000 u/g, extraction temperature 45°C, and liquid-to-solid ratio 30 ml/g. The experimental yield of crude TFP was 6.58%, which was very close to the predicted yield of 6.71%. TFPI was then purified and characterized with Sephadex G-100 column, UV-Vis, GPC, and FT-IR. The average molecular weight of TFPI was calculated to be 1.49 × 105 Da. TFPI exhibited strong reducing power and possessed not only remarkable scavenging activities against ABTS•+ and DPPH radicals, but also high antitumor activities in C4-2, DU145, and PC3 cells. The results suggest that Trichosanthes Fructus and TFPI could be a novel potent natural medicine with antioxidant and antitumor activities.

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Optimization of polysaccharides from Lycium ruthenicum fruit using RSM and its anti-oxidant activity

Cited by 112 publications

References 41 publications

Optimization of ultrasound‐assisted extraction of functional polysaccharides from common mullein (Verbascum thapsus L.) flowers

Optimization of ultrasound‐assisted extraction of functional polysaccharides from common mullein (Verbascum thapsus L.) flowers

Fatty Acid and Phytosterol Composition, and Biological Activities of Lycium ruthenicum Murr. Seed Oil

Polysaccharides fromTrichosanthes Fructusvia Ultrasound-Assisted Enzymatic Extraction Using Response Surface Methodology

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