Optimization of Preservation Methods Provides Insights into Photosynthetic Picoeukaryotes in Lakes

Liu, Changqing; Jin, Lan; Zhang, Min; Wu, Fan; Ren, Mingdong; Yang, Jiang-Yan; Wu, Qinglong L.; Shi, Xiaoli

doi:10.1128/spectrum.02557-21

Cited by 2 publications

(1 citation statement)

References 56 publications

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“…The following supporting information can be downloaded at: https: //www.mdpi.com/article/10.3390/agrochemicals3010001/s1. Table S1: Properties of Pluronics used in studies with PcO6; Table S2: Cell types to which Pluronic -F68 can serve as a cryoprotective agent under various research conditions [38][39][40][41][42];…”

Section: Supplementary Materialsmentioning

confidence: 99%

Adjuvant Pluronic F68 Is Compatible with a Plant Root-Colonizing Probiotic, Pseudomonas chlororaphis O6

Streeter,

Cartwright,

Zargaran

et al. 2023

Agrochemicals

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Plant probiotic bacteria are being increasingly used to maximize both the productivity and quality of field crops. Pseudomonas chlororaphis O6 (PcO6) is a plant root colonizer with probiotic activities. This bacterium produces an array of metabolites, including a group of phenazines that are functional in plant protection. The paper reports responses of PcO6 to a nonionic triblock copolymer surfactant, Pluronic F68. This Pluronic exhibits membrane “healing” activity and improves cryopreservation recovery in eukaryotic cells. The product is FDA-approved and is applied as an adjuvant in formulations used in agriculture, medicine, and biotechnology. Growth of PcO6 on lysogeny broth at 25 °C was unhindered by 0.1 and 1.0 g/L F68, reduced at 10 g/L, and with significant inhibition at 100 g/L F68; micelle formation could account for inhibited growth at higher doses. Phenazine production was not changed by F68, whereas the surfactant activity of F68 induced the spread of bacterial colonization on 0.5% agar. Exposure of cells to fluorescein-labeled F68 resulted in intense fluorescence, stable to washing, showing a direct association of the Pluronic with the bacterium. However, neither protection nor harm was found for PcO6 cells suspended in either 0.1% or 1% F68 after three freeze (−20 °C)/thaw cycles. These findings suggest that F68 could be compatible for use in agricultural formulations with little effect on probiotics such as PcO6.

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Section: Supplementary Materialsmentioning

confidence: 99%

Adjuvant Pluronic F68 Is Compatible with a Plant Root-Colonizing Probiotic, Pseudomonas chlororaphis O6

Streeter,

Cartwright,

Zargaran

et al. 2023

Agrochemicals

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High‐frequency sampling captures variability in phytoplankton population‐specific periodicity, growth, and productivity

Hynes,

Winter,

Berthiaume

et al. 2024

Limnology & Oceanography

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The Hawaii Ocean Time‐series (HOT) at Station ALOHA (22.75°N, 158°W) in the North Pacific Subtropical Gyre (NPSG) serves as a critical vantage point for observing plankton biomass production and its ecological implications. However, the HOT program's near‐monthly sampling frequency does not capture shorter time scale variability in phytoplankton populations. To address this gap, we deployed the SeaFlow flow cytometer for continuous monitoring during HOT cruises from 2014 to 2021. This approach allowed us to examine variations in the surface abundance and cell carbon content of specific phytoplankton groups: the cyanobacteria Prochlorococcus, Synechococcus, and Crocosphaera as well as a range of small eukaryotic phytoplankton ( 5 μm). Our data showed that daily to monthly variability in Prochlorococcus and Synechococcus abundance matches seasonal and interannual variability, while small eukaryotic phytoplankton and Crocosphaera showed the highest seasonal and interannual fluctuations. The study also found that eukaryotic phytoplankton and Crocosphaera had higher median cellular growth rates (0.076 and , respectively) compared to Prochlorococcus and Synechococcus (0.037 and , respectively). These variances in abundance and growth rates indicate that shifts in the community structure significantly impact primary productivity in the NPSG. Our results underscore the importance of daily to monthly phytoplankton dynamics in ecosystem function and carbon cycling.

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Optimization of Preservation Methods Provides Insights into Photosynthetic Picoeukaryotes in Lakes

Abstract: PPEs contribute significantly to primary productivity in freshwater ecosystems. The combination of FCM sorting and high-throughput sequencing has been shown to be a powerful approach and can largely improve our view of the PPE diversity.

Cited by 2 publications

References 56 publications

Adjuvant Pluronic F68 Is Compatible with a Plant Root-Colonizing Probiotic, Pseudomonas chlororaphis O6

Adjuvant Pluronic F68 Is Compatible with a Plant Root-Colonizing Probiotic, Pseudomonas chlororaphis O6

High‐frequency sampling captures variability in phytoplankton population‐specific periodicity, growth, and productivity

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