Optimization of Production Parameters for Probiotic Lactobacillus Strains as Feed Additive

Abstract

In animal nutrition, probiotics are considered as desirable alternatives to antibiotic growth promoters. The beneficial effects of probiotics primarily depend on their viability in feed, which demands technical optimization of biomass production, since processing and storage capacities are often strain-specific. In this study, we optimized the production parameters for two broiler-derived probiotic lactobacilli (L. salivarius and L. agilis). Carbohydrate utilization of both strains was determined and preferred substrates that boosted biomass production in lab-scale fermentations were selected. The strains showed good aerobic tolerance, which resulted in easier scale-up production. For the freeze-drying process, the response surface methodology was applied to optimize the composition of cryoprotective media. A quadratic polynomial model was built to study three protective factors (skim milk, sucrose, and trehalose) and to predict the optimal working conditions for maximum viability. The optimal combination of protectants was 0.14g/mL skim milk/ 0.08 g/mL sucrose/ 0.09 g/mL trehalose (L. salivarius) and 0.15g/mL skim milk/ 0.08 g/mL sucrose/ 0.07 g/mL (L. agilis), respectively. Furthermore, the in-feed stabilities of the probiotic strains were evaluated under different conditions. Our results indicate that the chosen protectants exerted an extensive protection on strains during the storage. Although only storage of the strains at 4 °C retained the maximum stability of both Lactobacillus strains, the employed protectant matrix showed promising results at room temperature.

Enterococcus faecalis Is a Better Competitor Than Other Lactic Acid Bacteria in the Initial Colonization of Colon of Healthy Newborn Babies at First Week of Their Life

“…This Lactobacilli was previously isolated from human vagina ( Ocana et al, 1999 ) suggesting that inoculation of newborn babies by this bacterium might occur during normal delivery through the vagina. Some strains of L. agilis was suggested as health beneficial strains that can be applied as a feed additive for poultry ( Lan et al, 2003 ; Ren et al, 2019 ) and was considered as one of the vaginal Lactobacilli potential antagonistic against Candida spp. ( Gil et al, 2010 ).…”

Current Perspectives and Potential of Probiotics to Limit Foodborne Campylobacter in Poultry

“…In order to meet the needs of industrial production, the viability and persistence of probiotics under storage conditions such as lyophilization need to be evaluated as a practical consideration following the in vivo studies ( Blajman et al, 2015 ). The in-feed stability and viability of probiotics will ensure that a sufficient level is administered to the host to deliver the expected anti- Campylobacter effects under production conditions ( Ren et al, 2019 ).…”

Synergistic Effects of Probiotics and Phytobiotics on the Intestinal Microbiota in Young Broiler Chicken

“…The probiotic Lactobacillus cells were harvested from MRS broth after aerobic growth at 37 • C for 12 h. The pelleted biomass was concentrated by centrifugation (10 min, 15,000× g, 4 • C) and freeze-dried at -55 • C in a lyophilizer (LyoVac GT2, Hürth, Germany) for 48 h after pre-freezing at -80 • C as described previously [40]. The lyophilized probiotics were stored at 4 • C until mixed with the feed.…”