Optimization of Reflux Extraction Process for Phenylethanol Glycoside from Cistanche Deserticola by Response Surface Methodology

Abstract

This article takes the extraction rate of phenylethanol glycosides from Cistanche deserticola as an evaluation index to investigate the effects of factors such as ethanol temperature, time, volume fraction, solid-liquid ratio, and extraction times on the extraction effect. Response surface methodology is used to optimize the reflux extraction process conditions of phenylethanol glycosides from Cistanche deserticola, and the feasibility of the method is analyzed. The experimental results show that the solid-liquid ratio is 1; 10, the volume fraction of ethanol is 1:60, the extraction temperature is 80 ℃, and the average extraction rate of phenylethanol glycoside is 76.5880 mg/g. The relative error is 0.29%, with a small deviation. Based on the above results, the response surface methodology was applied to optimize the reflux extraction process of phenylethanol glycosides, laying a theoretical foundation for extracting active substances from Cistanche deserticola and industrial production [1] .