Optimization of Release Conditions for Acetylated Amino Sugars from Glycoprotein with the Aid of Experimental Design and Their Sensitive Determination with HPLC

Explorations in a galaxy of sialic acids: a review of sensing horizons, motivated by emerging biomedical and nutritional relevance

“…[27][28][29] However, recently Sun et al reported the presence of a considerable amount of Neu5Ac in pea seedlings. 30 Furthermore, Xia et al extracted Sia from the sialylated glycoprotein from the root of a Tibetan plant Potentilla anserina L. 31 Hence, the present state of art is not able to confirm that Sia is absent in all parts of the plants.…”

“…They could produce LDR 0.25-10.0 μM for both Neu5Ac and Neu5Gc with LOD 1.09 pmol (for Neu5Ac) and 1.06 pmol (for Neu5Gc). 31 451 The pre-derivatization with HCl-n-butanol and reversed phase isotope dilution mass-spectrometry in positive-mode ESI source and tandem mass-spectrometer showed LDR and LOD 2-1000 and 0.42 μM, respectively, for Neu5Ac. 452 Wang et al followed a similar strategy i.e., isotope dilution of Neu5Ac with 1,2,3-13 C 3 Neu5Ac, inclusion of ketodeoxynonulosonic acid as an internal standard followed by pre-derivatisation with 3,4-diaminotoluene and eluting through ultra-HPLC coupled with positive ion mode ESI source and multiple reaction monitoring with Qtrap MS/MS detector.…”

“…They could produce LDR 0.25–10.0 μM for both Neu5Ac and Neu5Gc with LOD 1.09 pmol (for Neu5Ac) and 1.06 pmol (for Neu5Gc). 31 Hayama et al pre-derivatised Neu5Ac and Neu5Gc with heptadecafluoroundecylamine in the presence of 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride and used fluorous-phase fluophase reversed-phase HPLC column coupled with positive mode ESI source and Qtrap tandem mass spectrometer to assay Neu5Ac and Neu5Gc in the LDR 1–100 μM (for Neu5Ac) and 0.05–10 μM (for Neu5Gc). 451 The pre-derivatization with HCl- n -butanol and reversed phase isotope dilution mass-spectrometry in positive-mode ESI source and tandem mass-spectrometer showed LDR and LOD 2–1000 and 0.42 μM, respectively, for Neu5Ac.…”