Optimization of scanning electron microscope technique for amniotic membrane investigation: A preliminary study

Shehadat, Saaid Al; Görduysus, Melahat; Hamid, Suzina Sheikh Abdul; Nurul, Asma Abdullah; Samsudin, Abdul Rani; Ahmad, Azlina

doi:10.4103/ejd.ejd_401_17

Cited by 52 publications

(40 citation statements)

References 19 publications

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“…FTIR is a tool for measuring the radiation absorption of infrared regions at various wavelengths. 18 This test is used to identify functional groups or collagen-chitosan compounds. The membrane sample was placed on a disk printer and then vacuumed to remove the gas content.…”

Section: Methodsmentioning

confidence: 99%

Fabrication and Characterization of Chitosan-Collagen Membrane from Barramundi (Lates Calcarifer) Scales for Guided Tissue Regeneration

et al. 2019

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Objectives The aim of this study was to evaluate the synthesis, mechanical strength, and morphology of chitosan–collagen membranes from barramundi scales for guided tissue regeneration technique. Materials and Methods Collagen was extracted from barramundi scales by immersion in acetic acid. The resulting wet collagen was later dried. The membrane was fabricated by mixing chitosan with collagen from barramundi scales. Membrane characterization parameters were measured using Fourier transform infrared (FTIR), scanning electron microscopy (SEM) and mechanical property. Results The FTIR spectrum showed the typical peak of the mixture of chitosan and collagen. The tensile strength and elongation at break of the membrane in dry condition were 0.28 MPa and 8.53%, respectively, while in the wet condition these were 0.12 MPa and 25.6%. The membrane porosity test result was 38.85%; SEM result showed a porous membrane surface with size varying around 16 to 100 µm. Conclusion The chitosan-collagen membrane from the barramundi scale showed the fibrous membrane surface that has ideal porous size as guided tissue regeneration membrane and the lower mechanical strength.

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Section: Methodsmentioning

confidence: 99%

Fabrication and Characterization of Chitosan-Collagen Membrane from Barramundi (Lates Calcarifer) Scales for Guided Tissue Regeneration

et al. 2019

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“…These images provide significant information about a sample's composition and surface topography. [12] Hence, SEM evaluation of residual dentin surface after caries excavation with two different CMCR agents was considered in this study. The dentinal substrate remaining after caries excavation is very important to maintain pulp vitality, for the adhesion of restorative material and to achieve bio-mimetic restoration.…”

Section: Discussionmentioning

confidence: 99%

Scanning Electron Microscopic Evaluation of Residual Dentin Surface in Primary Teeth After Using Two Chemo-mechanical Caries Removal Agents: An in vitro study

Thazhatheethil¹,

Hiremath²,

Sarakanuru³

et al. 2021

J Pediatr Dent

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Objective:The objective of the study was to evaluate and compare the residual dentin surface after chemo-mechanical caries excavation with Carie-careTM and Brix-3000 by scanning electron microscopy in primary molars. Materials and Methods:Extracted sixty deciduous molars with occlusal caries were collected and randomly assigned into two groups with thirty each. Group 1; samples were treated with Carie-careTM, Group 2; samples were treated with Brix-3000. Following caries excavation, the tooth specimens were subjected to scanning electron microscopic evaluation.Results: Scanning electron microscopic evaluation showed that the two agents were equally effective in removing the infected dentin and smear layer in primary teeth. However, on statistical comparison using Chi square test and Mann Whitney showed no significant differences in two groups. Conclusion:Both the chemo-mechanical caries removal agents were effective in caries removal in primary molars. Hence, Brix-3000 can be effectively used in primary teeth in clinical practice.

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“…[ 13 ] Good quality SEM images were obtained even without gold sputtering according to Shehadat et al . [ 14 ] A carbon tape was used to aid in the conduction in the present study.…”

Section: Discussionmentioning

confidence: 99%

Comparative evaluation of remineralizing potential of commercially available agents MI paste, Remin pro, and Clinpro using Scanning Electron Microscope and Energy Dispersive X-ray: An in vitro study

2020

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Background: According to recent approaches, the prevention of initial dental caries is achieved using certain noninvasive methods. One such method is the topical application of remineralizing agents. Various remineralizing products are commercially available, but their efficacy is uncertain. Aim: The aim of the study was to evaluate the remineralizing potential of MI, Reminpro, and Clinpro using scanning electron microscope (SEM) and energy dispersive X-ray (EDX). Methodology: Forty extracted human premolars were collected. The samples were decoronated and the crown portion was coated with nail varnish leaving behind a 3 mm × 3 mm window on the labial surface. The samples were soaked in demineralizing solution for 4 days to create an artificial lesion, followed by SEM-EDX analysis. The samples were then randomly divided into 4 groups Control, MI, Remin Pro, and Clinpro with ten samples each. The remineralizing agents were applied according to the group and subjected to pH cycling for 21 days which was followed by SEM-EDX analysis. Data obtained were statistically analyzed using SPSS IBM version 23 (ANOVA, post hoc -Tukey, and Paired- t -test). Results: Ca and P were increased after remineralization in all groups. Between groups, the increase was more for ReminPro, followed by Clinpro and MI. Conclusion: In this study, the remineralizing potential of Remin Pro was effectively more, followed by Clinpro and MI.

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Optimization of scanning electron microscope technique for amniotic membrane investigation: A preliminary study

Cited by 52 publications

References 19 publications

Fabrication and Characterization of Chitosan-Collagen Membrane from Barramundi (Lates Calcarifer) Scales for Guided Tissue Regeneration

Fabrication and Characterization of Chitosan-Collagen Membrane from Barramundi (Lates Calcarifer) Scales for Guided Tissue Regeneration

Scanning Electron Microscopic Evaluation of Residual Dentin Surface in Primary Teeth After Using Two Chemo-mechanical Caries Removal Agents: An in vitro study

Comparative evaluation of remineralizing potential of commercially available agents MI paste, Remin pro, and Clinpro using Scanning Electron Microscope and Energy Dispersive X-ray: An in vitro study

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