Optimization of solvent bar microextraction combined with gas chromatography for preconcentration and determination of methadone in human urine and plasma samples

Ebrahimzadeh, Homeira; Mirbabaei, Fatemeh; Asgharinezhad, Ali Akbar; Shekari, Nafiseh; Mollazadeh, Narges

doi:10.1016/j.jchromb.2013.12.011

Cited by 39 publications

(14 citation statements)

References 34 publications

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“…To increase the affinity of the MDN toward sorbent coating and enhance the extraction efficiency, the pH of solution should be controlled to keep MDN in its molecular form. Since MDN is a weak basic compound (p K a 8.25; Ebrahimzadeh, Mirbabaei, Asgharinezhad, Shekari, & Mollazadeh, ), at pHs lower than p K a , this analyte remains in its cationic form. The effect of sample pH on the extraction of MDN was studied through the pH range of 7–11.…”

Section: Resultsmentioning

confidence: 99%

Solid‐phase microextraction of methadone in urine samples by electrochemically co‐deposited sol–gel/Cu nanocomposite fiber

Mohammadiazar

Hasanli

Maham

et al. 2017

Biomedical Chromatography

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Electrochemically co-deposited sol-gel/Cu nanocomposites have been introduced as a novel, simple and single-step technique for preparation of solid-phase microextraction (SPME) coating to extract methadone (MDN) (a synthetic opioid) in urine samples. The porous surface structure of the sol-gel/Cu nanocomposite coating was revealed by scanning electron microscopy. Direct immersion SPME followed by HPLC-UV determination was employed. The factors influencing the SPME procedure, such as the salt content, desorption solvent type, pH and equilibration time, were optimized. The best conditions were obtained with no salt content, acetonitrile as desorption solvent type, pH 9 and 10 min equilibration time. The calibration graphs for urine samples showed good linearity. The detection limit was about 0.2 ng mL . Also, the novel method for preparation of nanocomposite fiber was compared with previously reported techniques for MDN determination. The results show that the novel nanocomposite fiber has relatively high extraction efficiency.

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Section: Resultsmentioning

confidence: 99%

Solid‐phase microextraction of methadone in urine samples by electrochemically co‐deposited sol–gel/Cu nanocomposite fiber

Mohammadiazar

Hasanli

Maham

et al. 2017

Biomedical Chromatography

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“…The analytical data, such as preconcentration factors (PFs), linear dynamic ranges (LDRs), regression equations, correlation coefficients ( r 2 ), the LODs, and extraction recoveries (ER%) were obtained. The preconcentration factor and extraction recovery (ER%) were calculated based on the following equations :

PF = \frac{c_{f}}{c_{i}}

(ER) % = (\frac{c_{f} \times v_{f}}{c_{i} \times V v_{i}}) = PF \times (\frac{V_{f}}{V_{i}})

…”

Section: Resultsmentioning

confidence: 99%

Surfactant-assisted dispersive liquid-liquid microextraction of nitrazepam and lorazepam from plasma and urine samples followed by high-performance liquid chromatography with UV analysis

et al. 2015

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Surfactant-assisted liquid-liquid microextraction followed by high-performance liquid chromatography with UV detection has been developed for the simultaneous preconcentration and determination of lorazepam and nitrazepam in biological fluids. In this study, an ionic surfactant (cetyltrimethyl ammonium bromide) was used as an emulsifier. The predominant parameters affecting extraction efficiency such as the type and volume of extraction solvent, the type and concentration of surfactant, sample pH, and the concentration of salt added to the sample were investigated and opted. Under the optimum conditions (extraction solvent and its volume, 1-octanol, 70 μL; surfactant and its concentration, 1 mL of ultra-pure water containing 2 mmol L cetyltrimethyl ammonium bromide; sample pH = 9 and salt content of 10% NaCl w/v), the preconcentration factors were obtained in the range of 202-241 and 246-265 for nitrazepam and lorazepam, respectively. The limits of quantification for both drugs were 5 μg L in water sample and 10 μg L in biological fluids with R values higher than 0.993. The suitability of the proposed method was successfully confirmed by the extraction and determination of the target drugs in human urine and plasma samples in the range of microgram per liter.

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“…14,24 Various sample preparation techniques have been developed in previous work for the extraction and concentration of methadone and codeine in distinct real samples. These methods include solid-phase extraction (SPE), 1 dispersive solid-phase microextraction (DSPME), 25 dispersive liquid-liquid microextraction (DLLME), 11,19 air-assisted emulsification liquid-liquid microextraction (DES-AAELLME), 12 ultrasound-assisted dispersive liquidliquid microextraction (UA-DLLME), 26 homogenous liquid-liquid microextraction (HLLME), 27 hollow fiber liquid-phase microextraction (HF-LPME), 28 solvent bar microextraction (SBME) 29 and electromembrane extraction (EME). 30 The mention methodologies have been utilized for the determination of MDN and COD in various biological matrices, such as urine, 11,12,18,[28][29][30] plasma or blood, 11,12,19,[28][29][30] saliva 11 and sweat.…”

Section: Optimization Of New Sample Preparation Technique For the Detmentioning

confidence: 99%

“…35,36 The process of desorption and the nature of polymeric extraction phase have enormous relation in SPME method drawbacks; in fact, low repeatability and batch-to-batch variation are the results of the utilization of polymer as an extraction phase. 29,37 The DLLME is defined for its simplicity, high speed of extraction, ease of operation, high extraction recovery with high enrichment factor and simple equipment requirement. [38][39][40] Moreover, the existed particles in the biological samples during sample/acceptor phase separation can sediment at the bottom of the DLLME used device or suspend on the surface of its filled solution.…”

Section: Optimization Of New Sample Preparation Technique For the Detmentioning

confidence: 99%

Optimization of New Sample Preparation Technique for the Determination of Methadone and Codeine in Plasma Sample by GC-FID

Jamil

2020

J. Braz. Chem. Soc.

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A continuous sample drop flow microextraction (CSDF-ME) technique was modified for the pretreatment of plasma samples in the determination of methadone and codeine. The limit of detection and linear range for methadone were 15.0 µg L −1 and 0.1-10.0 mg L −1 , respectively. The detection limit and linear range for codeine were 25.0 µg L −1 and 0.2-15.0 mg L −1 , respectively. The extraction recoveries were 41.6-52.1%, and the enrichment factors ranged from 65 to 80 for codeine and methadone, respectively. Intra-and interday precisions of the technique were calculated for codeine and methadone concentrations of 0.4 mg L −1 and were 4.2-7.6% and 2.5-5.1%, respectively. Also, the accuracy (error%) of method was in the range −10 to +7.5%. The applicability of the CSDF-ME-gas chromatography-flame ionization detector (GC-FID) technique was illustrated by the determination of the mentioned drugs in human plasma. The attained relative recoveries of methadone and codeine in the 90.0-107.5% range demonstrated the excellent capability of the optimized technique for the microextraction of the mentioned drugs from plasma. The CSDF-ME-GC-FID is a rapid, repeatable and very simple technique that requires a low sample amount.

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Optimization of solvent bar microextraction combined with gas chromatography for preconcentration and determination of methadone in human urine and plasma samples

Cited by 39 publications

References 34 publications

Solid‐phase microextraction of methadone in urine samples by electrochemically co‐deposited sol–gel/Cu nanocomposite fiber

Solid‐phase microextraction of methadone in urine samples by electrochemically co‐deposited sol–gel/Cu nanocomposite fiber

Surfactant-assisted dispersive liquid-liquid microextraction of nitrazepam and lorazepam from plasma and urine samples followed by high-performance liquid chromatography with UV analysis

Optimization of New Sample Preparation Technique for the Determination of Methadone and Codeine in Plasma Sample by GC-FID

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